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Lyo-Ready Direct DNA and RNA/DNA LAMP Saliva Master Mixes

Lyo-Ready Direct DNA and RNA/DNA LAMP Saliva mixes are glycerol-free mixes that contain optimized excipients compatible with lyophilization and they are designed for direct loop mediated isothermal amplification for saliva testing of DNA and RNA.

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Meridian's Direct DNA and RNA/DNA LAMP Saliva Master Mixes

  • Develop ultra-sensitive LAMP saliva tests from crude saliva or sputum samples.
  • 反应快速,免核酸提取
  • Simply the development of POC saliva testing by just adding primers and probes.
  • Can be used wet or it lyophilized, ideal for pathogens at very low titers, such as viral pathogens.

在高至60%唾液中维持高效性能

唾液中含有激素、抗体、生长因子、酶和微生物等,可以用作疾病检测的生物标志物,是一种理想的诊断样本。并且,由于其无创、易采集、易运输的特点,成为POC检测的首选样本类型。但是由于分析物浓度低,PCR抑制物浓度高,唾液样本在分子诊断中的应用受到一定限制。 等温扩增技术,如LAMP,对抑制物具有很强的耐受性,且操作简单,可以实现更快速、灵敏和可靠的临床诊断结果。 迈迪安全新研发的可冻干唾液直扩LAMP/RT-LAMP预混液是分子诊断的创新性原料试剂。它将高度抑制物耐受的配方运用到了等温扩增体系中,从而实现对粗裂解样本的直接检测。该试剂独特的优化配方克服了唾液和痰液中存在的抑制物,免除了对患者样本纯化和提取DNA/RNA的步骤。此外,该试剂含有为冷冻干燥优化的冻干赋形剂,可用于开发常温稳定的干燥型检测。

Lyo-Ready Direct DNA LAMP Saliva, MDX134

Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots


Lyo-Ready Direct RNA/DNA LAMP Saliva, MDX135

Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots

Frequently Asked Questions

LAMP Saliva Testing

Saliva testing allows painless, non-invasive sample collection from the patient, making it ideal for use with children or patients that will not comply with blood collections or even intrusive nasal swabs. Saliva and sputum tests also increase the efficiency of collection while minimizing sample handling and reducing handling errors.

Saliva is a complex fluid containing various hormones, antibodies, growth factors, enzymes and microbes. Its composition virtually reflects the entire state of health and disease in a body, and it has the potential of being a diagnostic medium for a broad range of diseases, such as cancer and infectious agents, CMV, HIV, respiratory viruses (avian influenza viruses and coronaviruses) and bacterial infections.

Isothermal amplification mixes, particularly LAMP mixes generally tend to be reasonably inhibitor tolerant from a wide range of sample types from blood to plants, however the level of resistance is normally quite low. In designing the saliva specific mixes, we have been able to increase the inhibitor tolerant which results in faster time-to-results and reduced non-template amplification (NTC), both of which increase the assay sensitivity.

You will have faster time to results, no extraction is necessary.
You will get better sensitivity, for low copy number samples.
Less cross contamination, everything is done in a single tube, so there is less chance of mixing labels or adding materials into the wrong tubes.
More convenient, the lyophilized assay can be stored at room temperature and the sample added directly and run.
Reduced cost, there are no extraction costs or cold storage and cold transport costs with a simple heater, it can be used as a point-of-care assay.

The main difference between isothermal amplification and qPCR is that in isothermal amplification, the amplification is achieved using a single constant temperature, while in the qPCR, amplification requires cycling between multiple temperatures on a thermocycler. Instead of melting DNA strands apart at high temperatures, isothermal amplification uses polymerases with high strand displacement activity, like Bst DNA polymerases. As these polymerases do not need multiple temperatures, they can amplify a target in less than an hour, and in some cases in as little time as 10 minutes.

Lyo-Ready DNA LAMP and RNA/DNA LAMP Saliva can both be used directly as a liquid mix, or lyophilized, without changing the sensitivity of the assay. The master mixes are 4x concentration, this means that in a 20 µL reaction, 5 µL the master mix and 1 µL of primers and probes are used allowing for up to 14 µL of saliva or sputum to be added, however if the master mix, primers, and probes are lyophilized, then up to 20 µL of saliva or sputum can be added.

This will depend on what you are testing for and the amount, for example with SARS-CoV-2 heating to 95°C for 10 minutes has been shown to be beneficial, particularly with low viral loads, however Zika virus does not need pre-treatment, whereas some bacteria may require strong pre-treatment (such as proteinase K and heat inactivation).

Yes, it is possible to designed multiplex LAMP assays using different primers and probes, however they can be difficult to design and optimize as each sequence requires three sets of primers.

Yes, the Lyo-Ready Direct DNA LAMP Saliva and Lyo-Ready Direct RNA/DNA LAMP Saliva can be used as a liquid mix or lyophilized and stored for up to 24 months without needing to change the reaction conditions and this drying/ambient temperature storage will not affect the sensitivity of the test.

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