Lyo-Ready™ Direct DNA and RNA/DNA qPCR Stool
Lyo-Ready™ 可冻干粪便直扩 qPCR /RT-qPCR 预混液是不含甘油、含有冻干赋形剂的优化配方,适用于从粪便样本中直接进行灵敏地DNA 或RNA检测。
Can also be used wet and/or with purified nucleic acid
Sensitive Detection in up to 20% Stool
Lyo-Ready™ Direct DNA qPCR Stool, MDX142
Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots
Lyo-Ready™ Direct RNA/DNA qPCR Stool, MDX143
Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots
产品资料
Lyo-Ready Direct DNA and RNA/DNA qPCR StoolLyo-Ready Direct DNA and RNA/DNA qPCR Stool
可风干预混液可风干预混液
AIR-DRYING MOLECULAR ASSAYS可风干分子检测快速使用指南
可风干血液直扩可风干血液直扩
FAQs: qPCR Stool Test
A quantitative PCR stool test can allow identification of enteric pathogens such as harmful bacteria, fungi, viruses, or parasites down to the genus or species level, fast and accurately in a few hours, rather than the culture-based stool testing that can take several days. It can also be used as a non-invasive method for screening autoimmune conditions and cancer.
The fecal occult blood test (FOBT) has been the most widely used gastrointestinal immunoassay to screen colorectal cancer (CRC) for over three decades, however, recent large-scale studies have shown that the FOBT lacks the sensitivity to detect early stages of CRC. A qPCR cancer stool test is a better diagnostic tool as it offers significant improvement in sensitivity and accuracy and can be used to screen for multiple targets and so speed up diagnosis, therefore improving prognosis.
We have used up to 25% human stool with our mix, this is homogenized, and clarified by centrifugation and some pre-treatment may be required.
市场上有几种可用于粪便检测的缓冲液,包括磷酸盐缓冲盐水(PBS)、Cary-Blair储存液、粪便运输和复原(STAR)缓冲液和DNA/RNA Shield。
冻干后的预混液在正确封存的情况下可在常温下稳定保持2年。
Yes, most of it comes from cells that are sloughed into the lumen after cells die and are replaced, these cells go through apoptosis, releasing the DNA. Tumor cells may be present, but they will normally be less than 1% of the total human DNA fragments present in the overall stool sample.
Pre-treatment will depend on what you are looking at, cfDNA may not need pre-treatment, but you may need to heat to 95°C for 5 min and for some bacteria, a Proteinase K step (Proteinase K 55°C for 15 min and then at 98°C for 5 min) or using a non-ionic detergent (such as Tween-20 to final concentration of 0.2% followed by incubation at room temperature for 10 min) may be necessary.
在扩增DNA是我们没有发现这两种预混液之间的性能差异。™ Direct RT-qPCR Stool mix – Lyo-Ready™ Direct RNA/DNA qPCR Stool mix, as it can be used for both.
是的,Lyo-Ready™ Direct DNA qPCR Stool and Lyo-Ready™ Direct RNA/DNA qPCR Stool can be used as a liquid mix or lyophilization and stored for up to 24 months without needing to change the reaction conditions and lyophilization/ambient temperature storage will not affect the sensitivity of the test.
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