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Lyo-Ready Direct DNA and RNA/DNA qPCR Stool

Lyo-Ready 可冻干粪便直扩 qPCR /RT-qPCR 预混液是不含甘油、含有冻干赋形剂的优化配方,适用于从粪便样本中直接进行灵敏地DNA 或RNA检测。

Can also be used wet and/or with purified nucleic acid


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Meridian's Lyo-Ready Direct DNA and RNA/DNA qPCR Stool Mixes

  • 对粪便样本进行直接qPCR检测

  • 简化对粪便样本定量PCR检测的研发过程,免除核酸提取步骤,简化样本处理检测的流程

  • 经验证可用于粪便病原体检测和液体活检检测

  • 可用于液体或冻干干燥形式的检测,可用来开发常温稳定的检测

Sensitive Detection in up to 20% Stool

qPCR Stool graphs

Lyo-Ready Direct DNA qPCR Stool, MDX142

Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

 

Lyo-Ready Direct RNA/DNA qPCR Stool, MDX143

Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

粪便中存在的PCR抑制物,如胆盐、多糖、血红素和代谢产物,是DNA/RNA直扩的挑战。此外,由于粪便样本的高度复杂性和异质性,通常需要对样本进行预处理以去除其中可能存在的干扰物质,如食物残渣、微生物、脱落的上皮细胞和粘液。 可冻干粪便直扩qPCR/RT- qPCR预混液是专门为粪便样本直扩开发的配方,可减少样本的处理步骤。该试剂含有优化的添加剂对抑制物有更强的耐受性,同时可保持样本的质量和完整性。 它具有更短样本检测周转时间、更高灵敏度以及更长保质期的特点,能满足对胃肠道疾病快速、无创检测日益增加的需求。 如果您正在开发寄生虫、病毒、细菌的感染性疾病检测,或炎症性肠病或胃癌或结肠癌的筛查检测,使用可冻干粪便直扩qPCR /RT-qPCR 预混液能缩短检测开发的时间, 只需要加入 引物探针即可。 该产品既可以直接以液体形式使用,也可以冻干成常温稳定的干燥型试剂使用。

FAQs: qPCR Stool Test

A quantitative PCR stool test can allow identification of enteric pathogens such as harmful bacteria, fungi, viruses, or parasites down to the genus or species level, fast and accurately in a few hours, rather than the culture-based stool testing that can take several days. It can also be used as a non-invasive method for screening autoimmune conditions and cancer.

The fecal occult blood test (FOBT) has been the most widely used gastrointestinal immunoassay to screen colorectal cancer (CRC) for over three decades, however, recent large-scale studies have shown that the FOBT lacks the sensitivity to detect early stages of CRC. A qPCR cancer stool test is a better diagnostic tool as it offers significant improvement in sensitivity and accuracy and can be used to screen for multiple targets and so speed up diagnosis, therefore improving prognosis.

We have used up to 25% human stool with our mix, this is homogenized, and clarified by centrifugation and some pre-treatment may be required.

市场上有几种可用于粪便检测的缓冲液,包括磷酸盐缓冲盐水(PBS)、Cary-Blair储存液、粪便运输和复原(STAR)缓冲液和DNA/RNA Shield。

冻干后的预混液在正确封存的情况下可在常温下稳定保持2年。

Yes, most of it comes from cells that are sloughed into the lumen after cells die and are replaced, these cells go through apoptosis, releasing the DNA. Tumor cells may be present, but they will normally be less than 1% of the total human DNA fragments present in the overall stool sample.

Pre-treatment will depend on what you are looking at, cfDNA may not need pre-treatment, but you may need to heat to 95°C for 5 min and for some bacteria, a Proteinase K step (Proteinase K 55°C for 15 min and then at 98°C for 5 min) or using a non-ionic detergent (such as Tween-20 to final concentration of 0.2% followed by incubation at room temperature for 10 min) may be necessary.

在扩增DNA是我们没有发现这两种预混液之间的性能差异。 Direct RT-qPCR Stool mix – Lyo-Ready Direct RNA/DNA qPCR Stool mix, as it can be used for both.

是的,Lyo-Ready Direct DNA qPCR Stool and Lyo-Ready Direct RNA/DNA qPCR Stool can be used as a liquid mix or lyophilization and stored for up to 24 months without needing to change the reaction conditions and lyophilization/ambient temperature storage will not affect the sensitivity of the test.

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