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NGS High-Fidelity Pfu Buffer, 10x

文库扩增是通过PCR进行。 除了增加DNA的数量,PCR扩增还会对两端连接有接头的片段进行富集,因为这些片段将会附着在流动细胞上,并将成为序列。 PCR步骤中不引入图片至关重要,更低的错误率意味着更少的序列覆盖,节省了费用和时间。

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NGS High-Fidelity Pfu Buffer, 10x, MDX038

Available in 5 mL (1,000 Rxns) aliquots

二代测序高保真Pfu缓冲液,10x是一种含有dNTPs和Mg的优化缓冲液,只需要添加高保真聚合酶(如高保真Pfu (MDX003))、引物和DNA模板即可进行强大的扩增。

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