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Air Dryable™ Lyo Ready™ Enzymes

Air-Dryable & Lyo-Ready Enzymes

Meridian offers a selection of high-quality glycerol-free enzymes to help you develop highly sensitive assays. Removing the glycerol allows give assay developers the option for compatibility with lyophilization or air-drying, to dry down for stable ambient temperature shipment and storage. We also have high concentration enzymes that save on volume to allow more sample material to be added and gives greater flexibility in assay development strategies and allowing increase sensitivity, specificity, speed, stability, and enable a simplified workflow.

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High-Sensitivity Air Dryable and Lyo Ready Enzymes for Assays

  • Glycerol-free solution, for preparation of dried-down assays.
  • High concentration, allowing smaller reaction volumes and greater flexibility in assay optimization.
  • Ideal for POC microfluidic devices.

Meridian high-concentration, glycerol-free enzymes help you develop highly sensitive assays. They have been developed for speed and are designed for superior sensitivity and yield. The high concentration Being glycerol-free allows for the option of lyophilization or air-drying, allowing ambient temperature shipping and storage, and increased flexibility in sample volume.  

FAQ’s

Lyophilization is done by freezing and sublimation in a vacuum, forming a cake or bead, it has very low viscosity and up to 99% of the moisture is removed. Drying normally take 18-24 hours and requires cryo-excipients that protect the enzymes during the freezing. The reagents can be lyophilized to a bead or a cake format.

Air-drying is done by heating in a convection oven, forming a highly viscous pellet, with about 95% of the moisture removed. Drying normally take less than 2 hours and requires heat protecting excipients that protect the enzymes at higher temperatures. The reagents can be air-dried to a soft viscous format.

Lyophilization and air-drying helps to extend the product’s shelf life, enhance the stability of the reagents at higher temperatures, and allows for convenient and accurate dispensing of the reagents. After drying, the reagents are easily reconstituted with sample material.

Yes, reaction conditions are the same and they will produce the same results, this means that the liquid mixes can be used to create SOPs if you do not want to dry down and then later if drying is is required (for example to increase sensitivity), the SOP can be updated for drying, it does not require completely new SOPs to be written, but excipients will need to be added.

Following drying in the presence or absence of a buffer containing excipients, primers/probes, the mix should be stable for a minimum of 24 months at ambient temperature. Following rehydration, the assay reproducibility, sensitivity, and robustness should be the same as for a freshly made liquid mix.

No, our lyo-excipients are proprietary, if you require a buffer that contains lyo-excipients, we have the Lyo-Ready qPCR Buffer, 2.5x (MDX022).

High-concentration enzymes help reduce volumes when drying down, making the process faster, they also reduce volumes in microfluidic, cartridges and point of care (POC) devices, allowing for more sample to be added. They facilitate optimization and can speed up the reaction (as long as there is substrate available to bind to), increasing sensitivity and yield, as a result, they can be employed in a wide range of assay formats, allowing more flexibility for development and cost saving during commercialization.

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