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VLP-RNA Extraction Controls

Meridian’s VLP-RNA Extraction Controls offer a simple and effective way to reduce false-negative results in RT-qPCR assays. The biomimetic controls are composed of virus-like particle (VLP) shells packaged with a defined number of copies of target RNA molecules. Their composition closely resembles a test sample and serves as a full-process quantitative PCR positive control, monitoring the success of an RT-qPCR assay from lysis/extraction to reverse transcription and amplification.

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Meridian's VLP-RNA Extraction Control

  • Contains a defined number of copies of target RNA molecules, encapsidated within a virus-like particle (VLP).

  • Closely mimics the test sample, undergoing the same processing from lysis and extraction to RT-qPCR detection.

  • Non-infectious material for ease of handling and shipping.

  • Compatible with commonly used RNA extraction methods and lyophilization for creating freeze-dried mixes.

Why use VLP-RNA Extraction Controls in an RT-qPCR assay?

Possible causes of negative detection in an RT-qPCR
VLP-RNA Extraction Controls graph
Spike-In internal controls are RNA molecules added between the nucleic acid isolation and the detection, they do not monitor the extraction and lysis steps. Mimics internal controls are RNA molecules provided in a format resembling a biological sample (i.e. micelles) and are added just before lysis, but do not allow long incubation with the sample. VLP-RNA Extraction Control have better nuclease resistance, for long incubation with the sample, better quantification for consistent input in MDx processes and are easier to ship and store, ensuring that the MDx process provides reliable results by questioning each step of the process from lysis, through extraction to detection.

Description

VLP-RNA Extraction Control can be customized with specific RNA sequences of your choice, up to a total of 1,000 nt, to work with your assay primers and probe to enable multiple controls within a single VLP. The detection of VLP-RNA Extraction Control acts as a PCR positive control and confirms the success of the extraction, reverse transcription, and amplification steps, avoiding the misinterpretation of a false negative result.

Specifications

Description An Internal Control RNA sequence of the customers choice, encapsidated in a virus-like particle, used to confirms the success of the extraction, reverse transcription, and amplification steps
Application one-step RT-qPCR
Sample type Tissue, cells
Presentation 1 vial
Storage -20 °C
Mix stability See outer label

VLP-RNA Extraction Controls contain an internal control RNA sequence, with no known homology to any organism, encapsidated in a virus-like particle. The VLP-RNA Extraction Control is spiked in the sample prior to RNA extraction. Following RNA extraction, VLP-RNA Extraction Control can be detected in RT-qPCR by adding the VLP Detection Mix (containing specific primers and probe (emission wavelength = 560nm or 670nm)) to the reaction mix. The detection of VLP-RNA Extraction Control acts as a PCR positive control and confirms the success of the extraction, reverse transcription, and amplification steps, avoiding the misinterpretation of a false negative result.

FAQs: VLP-RNA Extraction Control

The VLP-RNA Extraction Controls are in plant based VLPs, so there is no animal/human viruses used within the process, so there is no possibility of the VLP-RNA Extraction Control giving false positive results.

The primers and probe for the VLP-RNA Extraction Control are in the VLP Detection Mix, however, we do not supply the primers and probe for the custom VLP-RNA Extraction Control.

The two colors are for the fluorescent dyes on the VLP-RNA Extraction Control probe. VLP Detection Mix Red (Cy5 – emission wavelength = 670nm) and VLP Detection Mix Orange (HEX – emission wavelength = 555nm). This allows you to choose one that will fit with your existing protocol in a multiplex RT-qPCR assay.

No, the VLP-RNA Extraction Control will work with commercially available silica-membrane extraction kits and CHELEX matrices and has been tested on a wide range of qPCR platforms.

The primers and probe for the VLP-RNA Extraction Control are in the VLP Detection Mix for VLP-RNA Extraction Control Red, MDX068 and VLP-RNA Extraction Control Orange, MDX069. However we do not supply the primers and probe for the custom VLP-RNA Extraction Control, MDX071.

The two colors are for the fluorescent dyes on the VLP-RNA Extraction Control probe. VLP Detection Mix Red (Cy5 – emission wavelength = 670nm) and VLP Detection Mix Orange (HEX – emission wavelength = 555nm). This allows you to choose one that will fit with your existing protocol in a multiplex RT-qPCR assay.

No, the VLP-RNA Extraction Control will work with commercially available silica-membrane extraction kits and CHELEX matrices and has been tested on a wide range of qPCR platforms.

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