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Buffers

Bst DNA Polymerase is an enzyme derived from the large fragment of Bacillus stearothermophilus DNA Polymerase I. It contains 5´- 3´ DNA polymerase activity and strong strand displacement activity but lacks 5´- 3´ exonuclease activity. The strong strand displacement activity enables Bst DNA Polymerase to synthesize DNA at a constant temperature making it an ideal enzyme for isothermal amplification, including HDA, MCA, and for Loop-Mediated Isothermal DNA Amplification (LAMP).

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High Conc. Glycerol-Free Bst, MDX018

Application: LAMP Specimen Type: Isothermal DNA Polymerase,Bst Enzymes & Buffers Concentration: 100 units/mL High Concentration: Yes Wet: Yes
Glycerol-Free: Yes Lyo-Ready: Yes Air-Dryable: Yes Dryable: Requires excipients Sustainability: Glycerol-Free

High concentration glycerol-free DNA polymerase (exo-), with strand-displacement properties. used for Isothermal DNA amplification such as LAMP (Loop-mediated Isothermal Amplification).

Available in 80 ‘L or 800 ‘L aliquots

Bst Reaction Buffer, 10x, MDX076

Application: LAMP Specimen Type: Buffer,Bst Enzymes & Buffers Concentration: 10x High Concentration: No Wet: Yes
Glycerol-Free: Yes Lyo-Ready: Requires excipients Air-Dryable: Requires excipients Dryable: Requires excipients Sustainability: Glycerol-Free

Reaction Buffer optimized for use with Bst DNA Polymerase.

Available in 5 mL or 100 mL aliquots

Enzyme Dilution Buffer, 1x, MDX078

Application: PCR Specimen Type: Buffer,Bst Enzymes & Buffers Concentration: 1x High Concentration: No Wet: Yes
Glycerol-Free: No Lyo-Ready: No Air-Dryable: No Dryable: No Sustainability:

Glycerol containing1x dilution buffer, for the dilution of enzymes to reaction concentration.

Available in 5 mL or 100 mL aliquots

Enzyme Dilution Buffer, 10x (Glycerol free), MDX080

Application: PCR Specimen Type: Buffer,Bst Enzymes & Buffers Concentration: 10x High Concentration: No Wet: Yes
Glycerol-Free: Yes Lyo-Ready: Yes Air-Dryable: Yes Dryable: Air-Dryable,Lyo-Ready Sustainability: Glycerol-Free

Glycerol-free,10x dilution buffer, for the dilution of enzymes to reaction concentration.

Available in 5 mL or 100 mL aliquots

Inhibitor-Tolerant Bst Buffer, MDX019

Application: LAMP Specimen Type: Buffer,Bst Enzymes & Buffers Concentration: 10x High Concentration: No Wet: Yes
Glycerol-Free: Yes Lyo-Ready: Yes Air-Dryable: Yes Dryable: Requires excipients Sustainability: Glycerol-Free

Inhibitor-Tolerant Bst Buffer is specially designed to overcome the inhibition present in samples in direct LAMP reactions.

Available in 2 mL or 100 mL aliquots

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Isothermal amplification such as loop-mediated isothermal amplification (LAMP) is a DNA amplification technique that can be performed at a single temperature. LAMP is currently considered one of the most powerful isothermal amplification techniques, relying on a strand-displacement polymerase combined with four to six primers. These primers recognize several specific regions in the target DNA and two of the primers form loop structures to facilitate subsequent rounds of amplification producing high levels of DNA.

Meridian’s Bst polymerases have strong strand displacement activity, fast polymerization, and enhanced inhibitor and salt tolerance when used with the specialized Inhibitor-Tolerant reaction buffer. The 100x high-concentration, glycerol-free BST enzyme was specifically designed to maximize assay flexibility for LAMP diagnostic test development.

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Have questions about a product? Want to learn more about Meridian’s molecular or immunoassay reagent portfolio? We want to hear from you!


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