Close

Loop Mediated Isothermal Amplification (LAMP)

Loop-mediated isothermal amplification (LAMP) is a DNA amplification technique that has played an increasingly important role in point-of-care (POC) diagnostics. It offers several advantages over qPCR and other amplification techniques in terms of sample-to-answer time, sensitivity, specificity, cost, robustness, and accessibility, making it ideal for field-deployable diagnostics in resource-limited regions. LAMP-based assays have been used for numerous applications including the detection of pathogens such as salmonella and malaria, GM crop contamination, and forensics to specifically detect human DNA.

LAMP uses four to six primers that recognize several specific regions in the target DNA. The reaction is initiated by a strand-displacement polymerase such as Bst and two specially designed primers which form loop structures to facilitate subsequent rounds of amplification producing high levels of DNA. LAMP reactions are very robust and inhibitor-tolerant, producing extremely large amounts of DNA that can be visualized by the eye (e.g. as a precipitate or color change). Specifically, 1 to 10 copies of DNA can be amplified to 109 to 1010 copies within 30 minutes, producing assays with excellent sensitivity and specificity.

MeridianSmartSearch

Use Meridian's Smart Search to find your product by entering a disease, product name, or catalog number in the below:

Not seeing what you’re looking for? Inquire about a new product

Why We Are Second To None

Meridian offers diagnostic manufacturers the complete solution with over 3,000 antigens and antibodies covering more than 500 diseases and an extensive range of specialty qPCR/RT-qPCR master mixes and molecular enzymes all manufactured under ISO13485 regulations.

  • Path Copy
    Large Sample Size
  • Lot Reservation
    Lot Reservation
  • ISO13485
    ISO13485
  • World-Class Customer Service
    World-Class Customer Service

Get In Touch With A Specialist

Have questions about a product? Want to learn more about Meridian’s molecular or immunoassay reagent portfolio? We want to hear from you!

By submitting your information in this form, you agree that your personal information may be stored and processed in any country where we have facilities or service providers, and by using our “Contact Us” page you agree to the transfer of information to countries outside of your country of residence, including to the United States, which may provide for different data protection rules than in your country. The information you submit will be governed by our Privacy Statement.

Learn More About Us

About

Welcome to

Please select your country to continue

Continue
EN