NGS Miniaturization

DNA sample preparation for next-generation sequencing (NGS) involves several steps. Firstly a DNA library is created that is suitable for the specific NGS platform to be used. This may have a target enrichment step prior to the addition of adaptors and amplification, with the quality of the library being depend on the nature of the DNA sample, input volume and level of difficulty (samples with high AC- or GC-rich regions). Each step having the potential to introduce errors or biases that could significantly impact library quality.

Meridian offers molecular reagents for library preparation that help reduce errors and bias to a minimum whilst allowing for efficiency and sensitivity.

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Name	Details	Application	Catalog	Order a Sample	Sizes	Description	Wet(Liquid)	Air-Dryable	Name	Glycerol-Free	Lyo-Ready	Concentration	High Concentration	Detail	Safety Data Sheet	COA/Test Release	Product Handling Guide	Product Manual
Gycerol-free T4 DNA Ligase (HC)	NGS Enzymes	NGS	MDX200	Order a Sample	Available in 1,000 Unit or 10,000 Unit aliquots	Glycerol-free, high concentration enzyme that efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. Ideal for lyophilization.	Yes	Requires excipients	Requires excipients	Yes	Requires excipients	50 Weiss unit/µL	Yes	View Details	Safety Data Sheet	COA/Test Release	Product Handling Guide
Glycerol-Free High-Fidelity Pfu (HC)	DNA Polymerase, NGS Enzymes	PCR	MDX203	Order a Sample	Available in 0.025 mL (500 Units) or 1 mL (20,000 Units) aliquots	Glycerol-free, high concentration, high fidelity DNA polymerase with separate dilution and reaction buffer, ideal for NGS library amplification and gene expression. Ideal for lyophilization.	Yes	Requires excipients	Requires excipients	Yes	Requires excipients	20 U/µL	Yes	View Details		COA/Test Release	Product Handling Guide
Glycerol-Free T4 Polynucleotide Kinase (HC)	NGS Enzymes	NGS	MDX206	Order a Sample	Available in 50 µL (500 Units) or 500 µL (5,000 Units) aliquots	Glycerol-free enzyme used to phosphorylate the ends of both DNA and RNA during labeling and ligation, mainly used in next-generation sequencing (NGS) library preparation. Ideal for lyophilization.	Yes	Requires excipients	Requires excipients	Yes	Requires excipients	20 U/µL	Yes	View Details	Safety Data Sheet	COA/Test Release	Product Handling Guide
Glycerol-Free T4 DNA Polymerase (HC)	NGS Enzymes	NGS	MDX207	Order a Sample	Available in 50 µL (500 Units) or 500 µL (5,000 Units) aliquots	Glycerol-free, high concentration DNA polymerase used for generating blunt ends on any duplex DNA molecule, used in next-generation sequencing (NGS) library preparation. Ideal for lyophilization.	Yes	Requires excipients	Requires excipients	Yes	Requires excipients	20 U/µL	Yes	View Details	Safety Data Sheet	COA/Test Release	Product Handling Guide
Glycerol-Free DNA Pol I Klenow Fragment (HC)	NGS Enzymes	NGS	MDX208	Order a Sample	Available in 200 µL (10,000 Units) or 1 mL (50,000 Units) aliquots	Glycerol-free, high concentration DNA polymerase used for generating blunt ends on any duplex DNA molecule, used in next-generation sequencing (NGS) library preparation. Ideal for lyophilization.	Yes	Requires excipients	Requires excipients	Yes	Requires excipients	20 U/µL	Yes	View Details	Safety Data Sheet	COA/Test Release	Product Handling Guide
Glycerol-Free DNA Pol I Klenow Fragment exo- (HC)	NGS Enzymes	NGS	MDX218	Order a Sample	Available in 50 µL (10,000 Units) or 1 mL (50,000 Units) aliquots	Glycerol-free, high concentration DNA Pol I Klenow Fragment exo-, designed for dA- and dT-tailing for next-generation sequencing (NGS) adapter ligation and DNA sequencing by the Sanger method.	Yes	Requires excipients	Requires excipients	Yes	Requires excipients	20 U/µL	Yes	View Details	Safety Data Sheet	COA/Test Release	Product Handling Guide
Lyophilized NGS Library Preparation Kit (PCR-Free)	NGS Enzymes	NGS	MDX219-A	Order a Sample	Available as 8 reactions, with and without PCR amplification	Lyophilized components for end-repair and A-tailing, ligation, and optionally library amplification. These can be stored at ambient temperature and are ready to use, just add DNA solution.	No	No	Lyo-Ready	Yes	Requires excipients	N/A	No	View Details	Safety Data Sheet	COA/Test Release		Product Handling Guide
Lyophilized NGS Library Preparation Kit	NGS Enzymes	NGS	MDX219-B	Order a Sample	Available as 8 reactions, with and without PCR amplification	Lyophilized components for end-repair and A-tailing, ligation, and optionally library amplification. These can be stored at ambient temperature and are ready to use, just add DNA solution.	No	No	Lyo-Ready	Yes	Requires excipients	N/A	No	View Details	Safety Data Sheet	COA/Test Release		Product Handling Guide



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Wet(Liquid)
Air-Dryable
Glycerol-Free
Lyo-Ready
Concentration
High Concentration
Description






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NGS Miniaturization

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