Proteinase K is a highly active serine protease (MW 28,500 Da) isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of native RNA and DNA from tissues or cell lines. Because the solution is tested for the absence of RNases and DNases, it is especially suitable for isolating qPCR and RT-qPCR templates.
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Proteinase K Solution, MDX055
Available in 25 mL aliquots
Proteinase K Solution is an enzyme used to digest most proteins in molecular biological techniques. The enzyme may be used at 56 °C for up to 4 hours or 37 °C for overnight incubations. Proteinase K Solution is a stabilized stock solution (20 mg/mL), stabilized with a specially formulated buffer, and can be used directly from the freezer.
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