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Home » Life Science » Products » Molecular Reagents » Ready to Use Master Mixes
Ready to Use Master Mixes

Ready to Use Master Mixes

Meridian’s ready to use master mixes are a combination of high-quality enzymes and highly optimized buffer chemistry, designed for highly sensitive, specific, reproducible assay results. They offer the benefit of speed and convenience, due to the ease of integration and optimization of the formula, with pre-assay preparation time being drastically reduced.
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Name Details Application Catalog Order a Sample Sizes Description Wet(Liquid) Air-Dryable Name Glycerol-Free Lyo-Ready Concentration High Concentration Detail Safety Data Sheet COA/Test Release User Guide Product Handling Guide Product Manual
Air-Dryable Direct DNA LAMP Blood, 4x Blood, Plasma, Serum, LAMP Mixes RT-LAMP MDX126 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 10% whole blood, 10% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.

 Yes Yes Air-Dryable Yes No 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct RNA/DNA LAMP Urine, 4x Urine, LAMP Mixes RT-LAMP MDX155 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details COA/Test Release User Guide Product Handling Guide
Lyo-Ready Genotyping Direct qPCR Urine, 4x Urine, Lyo-Ready Mix SNP Genotyping MDX158 Order a Sample Available in 1,000 Unit or 10,000 Unit aliquots

Inhibitor tolerance to 20% urine. Compatible with all dual-label probe chemistries for detection of genetic variants, such as single nucleotide and copy number variants, to give highly reproducible allelic discrimination.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details Safety Data Sheet COA/Test Release Product Handling Guide
Lyo-Ready Genotyping Direct qPCR FFPE, 4x FFPE, Lyo-Ready Mix SNP Genotyping MDX168 Order a Sample Available in 1,000 Unit or 10,000 Unit aliquots

Better extraction buffers and greater tolerance to crude FFPE samples. Compatible with all dual-label probe chemistries for detection of genetic variants, such as single nucleotide and copy number variants, to give highly reproducible allelic discrimination.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details COA/Test Release User Guide Product Handling Guide
Glycerol-Free T7 RNA Polymerase (HC) NASBA NASBA MDX201 Order a Sample Available in 50,000 Unit or 500,000 Unit aliquots

Glycerol-free, high concentration DNA-dependant RNA Polymerase that requires a T7 promoter sequence, it an ideal enzyme for in-vitro synthesis of labeled or unlabeled RNA for research, diagnostic or clinical applications. Ideal for lyophilization.

 Yes Requires excipients Requires excipients Yes Requires excipients 1,000 U/µL Yes View Details COA/Test Release Product Handling Guide
Glycerol-Free RNase H (HC) Reverse Transcriptase, NGS Enzymes, NASBA RT-qPCR MDX202 Order a Sample Available in 100 µL (2,500 Unit) or 250 µL (12,500 Unit) aliquots

Glycerol-free, high concentration RNA-specific endonuclease that hydrolyzes the phosphodiester bonds of RNA, when hybridized to DNA. Ideal for lyophilization.

 Yes Requires excipients Requires excipients Yes Requires excipients 50 U/µL Yes View Details COA/Test Release Product Handling Guide
Lyo-Ready Direct RNA/DNA LAMP Blood, 4x Blood, Plasma, Serum, LAMP Mixes RT-LAMP MDX125 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 10% whole blood, 10% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details COA/Test Release User Guide Product Handling Guide
Lyo-Ready Genotyping Direct qPCR Blood, 4x Blood, Plasma, Serum, Lyo-Ready Mix SNP Genotyping MDX128 Order a Sample Available in 1,000 Unit or 10,000 Unit aliquots

Inhibitor tolerance to 10% whole blood, 20% plasma. Compatible with all dual-label probe chemistries for detection of genetic variants, such as single nucleotide and copy number variants, to give highly reproducible allelic discrimination.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details Safety Data Sheet COA/Test Release Product Handling Guide
Air-Dryable Direct DNA qPCR Saliva, 4x Saliva, Sputum, Air-Dryable Mix qPCR MDX130 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.

 Yes Yes Air-Dryable Yes No 4x Yes View Details Safety Data Sheet COA/Test Release Product Handling Guide
Air-Dryable Direct RNA/DNA qPCR Saliva, 4x Saliva, Sputum, Air-Dryable Mix RT-qPCR MDX131 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.

 Yes Yes Air-Dryable Yes No 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct DNA qPCR Saliva, 4x Saliva, Sputum, Lyo-Ready Mix qPCR MDX132 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct RNA/DNA qPCR Saliva, 4x Saliva, Sputum, Lyo-Ready Mix RT-qPCR MDX133 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct DNA LAMP Saliva, 4x Saliva, Sputum, LAMP Mixes LAMP MDX134 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct RNA/DNA LAMP Saliva, 4x Saliva, Sputum, LAMP Mixes RT-LAMP MDX135 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Air-Dryable Direct DNA qPCR Stool, 4x Stool, Air-Dryable Mix qPCR MDX140 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.

 Yes Yes Air-Dryable Yes No 4x Yes View Details Safety Data Sheet COA/Test Release Product Handling Guide
Air-Dryable Direct RNA/DNA qPCR Stool, 4x Stool, Air-Dryable Mix RT-qPCR MDX141 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 5% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.

 Yes Yes Air-Dryable Yes No 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct DNA qPCR Stool, 4x Stool, Lyo-Ready Mix qPCR MDX142 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct RNA/DNA qPCR Stool, 4x Stool, Lyo-Ready Mix RT-qPCR MDX143 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 5% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct DNA LAMP Stool, 4x Stool, LAMP Mixes LAMP MDX144 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct RNA/DNA LAMP Stool, 4x Stool, LAMP Mixes RT-LAMP MDX145 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 5% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready Genotyping Direct qPCR Stool, 4x Stool, Lyo-Ready Mix SNP Genotyping MDX148 Order a Sample Available in 1,000 Unit or 10,000 Unit aliquots

Inhibitor tolerance to 20% stool. Compatible with all dual-label probe chemistries for detection of genetic variants, such as single nucleotide and copy number variants, to give highly reproducible allelic discrimination.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details COA/Test Release Product Handling Guide
Air-Dryable Direct DNA qPCR Urine, 4x Urine, Air-Dryable Mix qPCR MDX150 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 60% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.

 Yes Yes Air-Dryable Yes No 4x Yes View Details Safety Data Sheet COA/Test Release Product Handling Guide
Air-Dryable Direct RNA/DNA qPCR Urine, 4x Urine, Air-Dryable Mix RT-qPCR MDX151 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.

 Yes Yes Air-Dryable Yes No 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct DNA qPCR Urine, 4x Urine, Lyo-Ready Mix qPCR MDX152 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct RNA/DNA qPCR Urine, 4x Urine, Lyo-Ready Mix RT-qPCR MDX153 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct DNA LAMP Urine, 4x Urine, LAMP Mixes LAMP MDX154 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details COA/Test Release User Guide Product Handling Guide
Air-Dryable qPCR Mix, 4x PCR, qPCR, Air-Dryable Mix qPCR MDX082 Order a Sample Available in 5 mL (500 Rxns) or 50 mL (10,000 Rxns) aliquots

Glycerol-free qPCR mix containing Taq polymerase, reaction buffer, dNTPs, MgCl2 and air-dry compatible excipients, ideal for developing ambient temperature, multiplex molecular assays.

 Yes Yes Air-Dryable Yes No 4x Yes View Details Safety Data Sheet COA/Test Release User Guide
Air-Dryable Direct DNA qPCR Blood, 4x Blood, Plasma, Serum, Air-Dryable Mix qPCR MDX092 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% whole blood, 20% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.

 Yes Yes Air-Dryable Yes No 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Air-Dryable 1-Step RT-qPCR Mix, 4x Air-Dryable Mix RT-qPCR MDX095 Order a Sample Available in 5 mL (500 Rxns) or 50 mL (10,000 Rxns) aliquots

Glycerol-free RT-qPCR mix containing Taq polymerase, reverse transcriptase, reaction buffer, dNTPs, MgCl2 and air-dry compatible excipients, ideal for developing ambient temperature, multiplex molecular assays.

 Yes Yes Air-Dryable Yes No 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready LAMP Mix, 4x LAMP Mixes LAMP MDX097 Order a Sample Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots

Glycerol-free mix for isothermal applications such as LAMP, containing Bst DNA polymerase, reaction buffer, dNTPs and lyo-compatible excipients, ideal for developing ambient temperature, LAMP assays.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details COA/Test Release User Guide Product Handling Guide
Inhibitor-Tolerant RT-qPCR Lo-ROX Mix, 4x Blood, saliva, urine, stool, tissue RT-qPCR MDX105 Order a Sample Available in 5 mL (500 Rxns) or 50 mL (10,000 Rxns) aliquots

Combination of the latest advances in buffer chemistry and PCR enhancers and stabilizers, together with high quality enzymes, dNTPs and MgCl2, for use in the presence of inhibitors.

 Yes No No No No 4x Yes View Details COA/Test Release Product Handling Guide
Lyo-Ready RT-LAMP 1-Step Mix, 4x LAMP Mixes RT-LAMP MDX108 Order a Sample Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots

Glycerol-free mix for isothermal applications such as RT-LAMP, containing Bst DNA polymerase, reverse transcriptase, reaction buffer, dNTPs and lyo-compatible excipients, ideal for developing ambient temperature, LAMP assays.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details COA/Test Release User Guide Product Handling Guide
Lyo-Ready dUTP 1-Step RT-qPCR Mix Lyo-Ready Mix, Lyo-Ready Mix RT-qPCR MDX113 Order a Sample Available in 10 mL (1,000 Rxns) or 100 mL (10,000 Rxns) aliquots

Glycerol-free mix containing Taq polymerase, RNase Inhibitor, reaction buffer, dNTP/dUTP mix, MgCl2 and lyo-excipients and separate reverse transcriptase , developed to remove background PCR product contamination.

 Yes No Lyo-Ready Yes Yes 2x No View Details COA/Test Release Product Handling Guide
Air-Dryable Direct DNA qPCR Plant, 4x Plant, Air-Dryable Mix qPCR MDX116 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

No need for extraction, compatible with 25% Plant lysate (SDS, NaOH). Perfect for multiplexing and fast cycling conditions for in-field qPCR testing. Used for genotyping and transgene or pathogenic detection.

 Yes Yes Air-Dryable Yes No 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
55C MMLV-RT Reverse Transcriptase, Air-Dryable & Lyo-Ready Enzymes RT-LAMP MDX117 Order a Sample Available in 50 µL (10,000 Units), 200 µL (40,000 Units) or 1 mL (200,000 Units) aliquot

Highly robust, thermostable reverse transcriptase, ideal for cDNA synthesis from RNA with a high secondary structure such as viral RNA genomes.

 Yes Requires excipients Requires excipients Yes Requires excipients 200 U/µL Yes View Details Safety Data Sheet COA/Test Release Product Handling Guide
Air-Dryable RT-LAMP 1-Step Mix, 4x LAMP Mixes RT-LAMP MDX118 Order a Sample Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots

Glycerol-free mix for isothermal applications such as RT-LAMP, containing Bst DNA polymerase, reverse transcriptase, reaction buffer, dNTPs and air-dryable excipients, ideal for developing ambient temperature, LAMP assays.

 Yes Yes Air-Dryable Yes No 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Air-Dryable DNA LAMP LAMP Mixes LAMP MDX119 Order a Sample Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots

Glycerol-free mix for isothermal applications such as LAMP, containing Bst DNA polymerase, reaction buffer, dNTPs and air-dryable excipients, ideal for developing ambient temperature, LAMP assays.

 Yes Yes Air-Dryable Yes No 4x Yes View Details COA/Test Release User Guide Product Handling Guide
Air-Dryable Direct RNA/DNA qPCR Blood, 4x Blood, Plasma, Serum, Air-Dryable Mix RT-qPCR MDX121 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 10% whole blood, 10% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.

 Yes Yes Air-Dryable Yes No 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct DNA qPCR Blood, 4x Blood, Plasma, Serum, Lyo-Ready Mix qPCR MDX122 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% whole blood, 20% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct RNA/DNA qPCR Blood, 4x Blood, Plasma, Serum, Lyo-Ready Mix RT-qPCR MDX123 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 10% whole blood, 10% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct DNA LAMP Blood, 4x Blood, Plasma, Serum, LAMP Mixes LAMP MDX124 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% whole blood, 20% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.

 Yes No Lyo-Ready Yes Yes 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready 1-Step RT-qPCR Buffer Buffer, Air-Dryable & Lyo-Ready Enzymes RT-qPCR MDX052 Order a Sample Available in 10 mL (1,000 Rxns) or 100 mL (10,000 Rxns) aliquots

Glycerol-free RT-qPCR reaction buffer containing lyo-excipients, designed to deliver highly reproducible, accurate RT-qPCR assay results under fast thermal cycling conditions.

 Yes No Lyo-Ready Yes Yes 2x No View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Hi-throughput dUTP qPCR Lo-ROX PCR, qPCR, dU Containing qPCR MDX060 Order a Sample Available in 10 mL (1,000 Rxns) or 100 mL (10,000 Rxns) aliquots

Contains Taq polymerase, reaction buffer, dNTP, MgCl2, stabilizers, PCR enhancers, and ROX dye. The dTTP has been substituted for dUTP, so that UDGase can be added to remove background PCR carryover product contamination.

 Yes No No No No 2x No View Details Safety Data Sheet COA/Test Release Product Handling Guide
Lyo-Ready qPCR Buffer w/o Excipients, 4x Buffer, Air-Dryable & Lyo-Ready Enzymes qPCR MDX061 Order a Sample Available in 2.5 mL (500 Rxns) or 100 mL (20,000 Rxns) aliquots

Glycerol-free qPCR reaction buffer that is designed to allow formulation optimization to freeze-drying methods by excipients selection.

 Yes Requires excipients Air-Dryable,Lyo-Ready Yes Requires excipients 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready 1-Step RT-qPCR Virus Mix Lyo-Ready Mix, Lyo-Ready Mix RT-qPCR MDX062 Order a Sample Available in 10 mL (1,000 Rxns) or 100 mL (10,000 Rxns) aliquots

Taq polymerase, RNase inhibitor, reaction buffer, dNTP, MgCl2 and lyo-excipients and separate reverse transcriptase, optimized for amplification of RNA with high secondary structure.

 Yes No Lyo-Ready Yes Yes 2x No View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Fast qPCR Mix, 5x PCR, qPCR qPCR MDX072 Order a Sample Available in 2 mL (500 Rxns) or 40 mL (10,000 Rxns) aliquots

The latest advances in buffer chemistry and PCR enhancers and stabilizers, together with a hot-start polymerase, dNTPs and MgCl2, for highly reproducible, accurate assay results under fast thermal cycling conditions.

 Yes No No No No 5x Yes View Details Safety Data Sheet COA/Test Release Product Handling Guide
Inhibitor-Tolerant qPCR Mix, 5x PCR, qPCR qPCR MDX073 Order a Sample Available in 2 mL (500 Rxns) or 40 mL (10,000 Rxns) aliquots

Combination of the latest advances in buffer chemistry and PCR enhancers and stabilizers, together with high quality enzymes, dNTPs and MgCl2, for use in the presence of inhibitors.

 Yes No No No No 5x Yes View Details Safety Data Sheet COA/Test Release Product Handling Guide
Bst Reaction Buffer, 10x Buffer, Bst Enzymes & Buffers LAMP MDX076 Order a Sample Available in 5 mL or 100 mL aliquots

Reaction Buffer optimized for use with Bst DNA Polymerase.

 Yes Requires excipients Requires excipients Yes Requires excipients 10x No View Details Safety Data Sheet COA/Test Release Product Handling Guide
Enzyme Dilution Buffer, 1x Buffer, Bst Enzymes & Buffers PCR MDX078 Order a Sample Available in 5 mL or 100 mL aliquots

Glycerol containing1x dilution buffer, for the dilution of enzymes to reaction concentration.

 Yes No No No No 1x No View Details Safety Data Sheet COA/Test Release Product Handling Guide
Enzyme Dilution Buffer, 10x (Glycerol free) Buffer, Bst Enzymes & Buffers PCR MDX080 Order a Sample Available in 5 mL or 100 mL aliquots

Glycerol-free,10x dilution buffer, for the dilution of enzymes to reaction concentration.

 Yes Yes Air-Dryable,Lyo-Ready Yes Yes 10x No View Details Safety Data Sheet COA/Test Release Product Handling Guide
Lyo-Ready 1-Step RT-qPCR Mix Lyo-Ready Mix RT-qPCR MDX024 Order a Sample Available in 10 mL (1,000 Rxns) or 100 mL (10,000 Rxns) aliquots

Glycerol-free Taq polymerase, reaction buffer, dNTP, MgCl2, and lyo-excipients, and separate thermostable reverse transcriptase, ideal for developing room temperature, lyophilized, multiplex qPCR assays.

 Yes No Lyo-Ready Yes Yes 2x No View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Low LOD 1-Step RT-qPCR Mix RT-PCR, RT-qPCR RT-qPCR MDX025 Order a Sample Available in 10 mL (1,000 Rxns) or 100 mL (10,000 Rxns) aliquots.

RT-qPCR mix a dNTP/dUTP mix, designed for stringent amplification of highly structured and very low copy-number RNA and DNA targets.

 Yes No No No No 2x No View Details Safety Data Sheet COA/Test Release Product Handling Guide
Low DNA qPCR Mix PCR, qPCR qPCR MDX030 Order a Sample Available in 5 mL (500 Rxns) or 100 mL (10,000 Rxns) aliquots

Highly purified qPCR master mix with stringent chemical hot-start properties and low bioburden, ideal for PCR of low-copy bacterial targets to avoid false-positive amplification.

 Yes No No No No 2x No View Details Safety Data Sheet COA/Test Release Product Handling Guide
Hi-throughput dUTP qPCR Mix PCR, qPCR, dU Containing qPCR MDX031 Order a Sample Available in 5 mL (500 Rxns) or 100 mL (10,000 Rxns) aliquots

qPCR mix containing Taq polymerase, reaction buffer, dNTP/dUTP mix and MgCl2, developed to remove background PCR product contamination.

 Yes No No No No 2x No View Details Safety Data Sheet COA/Test Release Product Handling Guide
Fast 1-Step RT-qPCR Mix RT-PCR, RT-qPCR RT-qPCR MDX032 Order a Sample Available in 5 mL (500 Rxns) or 100 mL (10,000 Rxns) aliquots

A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start polymerase, dNTPs and MgCl2 and separate reverse transcriptas, ideal for fast, accurate, highly reproducible RT-qPCR.

 Yes No No No No 2x No View Details Safety Data Sheet COA/Test Release Product Handling Guide
NGS Library Quantification PCR, qPCR NGS MDX039 Order a Sample Available in 500 Rxns

Fast qPCR Mix, pre-diluted DNA standards, primer mix and dilution buffer, designed to accurately measure the number of amplifiable DNA fragments in Illumina-based NGS libraries before loading into a flow cell.

 Yes No No No No 2x No View Details Safety Data Sheet Product Handling Guide
Lyo-Compatible MMLV-RT Reverse Transcriptase, Air-Dryable & Lyo-Ready Enzymes RT-qPCR MDX042 Order a Sample Available in 8 µL (1,000 Rxns) or 80 µL (10,000 Rxns) aliquots

Glycerol-free high concentration reverse transcriptase and dilution buffer. Can be used to create 1-step RT-qPCR mixes for subsequent lyophilization.

 Yes Requires excipients Requires excipients Yes Requires excipients > 165 U/µL Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
High-Specificity Pfu HS Mix PCR, qPCR NGS MDX006 Order a Sample Available in 0.025 mL (500 Units) or 1 mL (20,000 Units) aliquots

High concentration, high-fidelity Taq DNA polymerase used for the amplification of a next-generation sequencing (NGS) library.

 Yes No No No No 2x No View Details Safety Data Sheet COA/Test Release Product Handling Guide
Glycerol-Free Taq HS, 50 U/µL DNA Polymerase, Air-Dryable & Lyo-Ready Enzymes PCR MDX011 Order a Sample Available in 0.02 mL (1,000 Units) or 0.5 mL (25,000 Units) aliquots

Lyophilization-compatible high-performance Taq DNA polymerase, separate Taq antibody and enzyme dilution buffer, suitable for difficult, multiplex tests that require fast amplification, whilst maintaining high specificity and sensitivity.

 Yes Requires excipients Requires excipients Yes Requires excipients 50 U/mL Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Bst DNA Polymerase Isothermal DNA Polymerase, Bst Enzymes & Buffers LAMP MDX012 Order a Sample Available in 1 mL or 10 mL aliquots

DNA polymerase (exo-), with strand-displacement properties. used for Isothermal DNA amplification such as LAMP (Loop-mediated Isothermal Amplification).

 Yes No No No No 8 units/mL No View Details Safety Data Sheet COA/Test Release Product Handling Guide
Inhibitor-Tolerant qPCR Mix Blood, saliva, urine, stool, tissue qPCR MDX013 Order a Sample Available in 5 mL (500 Rxns) or 100 mL (10,000 Rxns) aliquots

Universal inhibitor tolerant qPCR master mix, for direct amplification from crude lysates or inhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood, sputum, saliva and stool.

 Yes No No No No 2x No View Details Safety Data Sheet COA/Test Release Product Handling Guide
Aptamer Taq HS (Glycerol-Free) DNA Polymerase, Air-Dryable & Lyo-Ready Enzymes PCR MDX015 Order a Sample Available in 20 ?L (1,000 Units) or 1 mL (50,000 Units) aliquots

Glycerol-free, high concentration hot-start Taq DNA polymerase, suitable for difficult, multiplex molecular tests that require fast amplification.

 Yes Requires excipients Requires excipients Yes Requires excipients 50 U/mL Yes View Details COA/Test Release User Guide Product Handling Guide
Inhibitor-Tolerant RT-qPCR Mix, 4x Blood, saliva, urine, stool, tissue, RT-PCR, RT-qPCR RT-qPCR MDX016 Order a Sample Available in 5 mL (500 Rxns) or 50 mL (10,000 Rxns) aliquots

Universal, high concentration, inhibitor tolerant RT-qPCR master mix, for direct amplification from crude lysates or inhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood, sputum, saliva and stool.

 Yes No No No No 4x No View Details Safety Data Sheet COA/Test Release Product Handling Guide
High Conc. Glycerol-Free Bst Isothermal DNA Polymerase, Bst Enzymes & Buffers LAMP MDX018 Order a Sample Available in 80 µL or 800 µL aliquots

High concentration glycerol-free DNA polymerase (exo-), with strand-displacement properties. used for Isothermal DNA amplification such as LAMP (Loop-mediated Isothermal Amplification).

 Yes Yes Requires excipients Yes Yes 100 units/mL Yes View Details Safety Data Sheet COA/Test Release Product Handling Guide
Inhibitor-Tolerant Bst Buffer Buffer, Bst Enzymes & Buffers LAMP MDX019 Order a Sample Available in 2 mL or 100 mL aliquots

Inhibitor-Tolerant Bst Buffer is specially designed to overcome the inhibition present in samples in direct LAMP reactions.

 Yes Yes Requires excipients Yes Yes 10x No View Details Safety Data Sheet COA/Test Release Product Handling Guide
Fast qPCR Mix PCR, qPCR qPCR MDX020 Order a Sample Available in 5 mL (500 Rxns) or 100 mL (10,000 Rxns) aliquots

The latest advances in buffer chemistry and PCR enhancers and stabilizers, together with a hot-start polymerase, dNTPs and MgCl2, for highly reproducible, accurate assay results under fast thermal cycling conditions.

 Yes No No No No 2x No View Details Safety Data Sheet COA/Test Release Product Handling Guide
Lyo-Ready qPCR Mix PCR, qPCR, Lyo-Ready Mix qPCR MDX021 Order a Sample Available in 5 mL (500 Rxns) or 100 mL (10,000 Rxns) aliquots

Glycerol-free Taq polymerase, reaction buffer, dNTP, MgCl2, and lyo-excipients, ideal for developing room temperature, lyophilized, multiplex qPCR assays.

 Yes No Lyo-Ready Yes Yes 2x No View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready qPCR Buffer, 2.5x Buffer, Air-Dryable & Lyo-Ready Enzymes qPCR MDX022 Order a Sample Available in 8 mL (500 Rxns) or 100 mL (12,500 Rxns) aliquots

Glycerol-free qPCR reaction buffer containing lyo-excipients, designed to deliver highly reproducible, accurate qPCR assay results under fast thermal cycling conditions.

 Yes No Lyo-Ready Yes Yes 2.5x No View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready qPCR Mix 2.6x PCR, qPCR, Lyo-Ready Mix qPCR MDX023 Order a Sample Available in 8 mL (500 Rxns) or 100 mL (12,500 Rxns) aliquots

Glycerol-free Taq polymerase, reaction buffer, dNTP, MgCl2, and lyo-excipients, ideal for developing room temperature, lyophilized, multiplex qPCR assays.

 Yes No Lyo-Ready Yes Yes 2.6x No View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide

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Molecular Master Mixes for PCR and Isothermal Amplification

They also ensure consistent results, with fewer pipetting steps and lower likelihood of mistakes or contamination and variability between wells and allowing more samples to be run in a day with the highest confidence, ideal for high-throughput assays.

  • Fast cycling, reducing assay times.
  • Superior sensitivity, specificity, and reproducibility. 
  • Lyophilization and air-dryable options. 
  • Compatible with automated, high-throughput instruments.

Meridian offers ready to use molecular master mixes for PCR and isothermal amplification, include master mixes formulated for direct use with crude lysates, and master mixes that can be lyophilized and air-dried.

FAQ’s

Using molecular master mixes are convenient, reduces assay development times and pre-empt possible errors in mixing, making them ideal for high-throughput applications.

Master mixes usually contain enzymes (hot start polymerase/reverse transcriptase), an optimized buffer containing PCR enhancers, dNTPs, MgCl2, only template, primers, probes (if being used), and water, to make up the volume, normally need to be added.

Our master mixes are compatible with most standard cycling conditions and can also be used with very fast cycling conditions, however some optimization may be required for specific assay development.

There are several other advantages of one-step reactions, these include limited sample handling and reduced bench time, which helps to decrease chances for pipetting errors and cross contamination between reverse transcription and qPCR steps. This method is quick to set up and makes processing multiple RNA samples easy (especially when using liquid handling robotics), when you are amplifying only a few genes of interest. It is therefore ideal for high throughput screening laboratories where only a few reverse transcription qPCR assays are run repeatedly, using well-established reaction conditions, with the added advantage that multiplex qPCR of the gene of interest and control genes can be done in single well, from same RNA sample.

Yes, our PCR master mixes are designed to be used in multiplex assays. It is also possible to create a multiplex LAMP assay using different primers and probes, however they can be difficult to design and optimize as each sequence requires three sets of primers.

Yes, the Lyo-Ready and Air-Dryable master mixes can be used as liquid mixes or dried and stored at ambient temperature without needing to change the reaction conditions and drying/storage will not affect the sensitivity of the test.

It is stable for up to 2 years at ambient temperature if correctly stored in sealed pouches.

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Have questions about a product? Want to learn more about Meridian’s molecular or immunoassay reagent portfolio? We want to hear from you!


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