Close

Specimen-specific Inhibitor Tolerant Master Mixes

A variety of different inhibitors inherent to clinical and environmental samples can negatively impact the sensitivity and accuracy of a molecular assay. Conventionally, to overcome this, specimens undergo extraction and processing prior to testing. However, extraction technologies are not 100% efficient which can impact the amount of target nucleic acid available for testing, and some inhibitors can co-elute following purification and induce false-negative assay results.

In developing universal inhibitor tolerant qPCR and RT-qPCR mixes, a compromise is required to accommodate all of the different types of inhibitors, this reduces sensitivity of the quantitative PCR. Meridian has therefore developed sample specific inhibitor tolerant qPCR and RT-qPCR formulations, designed to be used for direct amplification from crude lysates or inhibitor-rich samples either from blood, saliva, urine or stool. The ability of these mixes to tolerate the high level of PCR inhibitors present in these different sample types allows the development of simpler, faster multiplex qPCR assays that require minimal sample processing and fast turn-around times (TAT). This makes them ideal for liquid biopsy and point of care testing (POCT), where speed, sensitivity, and reproducibility are essential.

The mixes can be used as wet mixes or dried by lyophilization or air-drying, there are several advantages to this, including room temperature shipping and storage, extended shelf-life, and increased flexibility in the sample volume.

MeridianSmartSearch

Use Meridian's Smart Search to find your product by entering a disease, product name, or catalog number in the below:

Not seeing what you’re looking for? Inquire about a new product

Interested to learn more about COVID/Flu/RSV multiplex testing?

Download Our New Whitepaper
btn play

How to Air-Dry an Assay


See how easy it is to Air-Dry an assay.

Click the play button on the left.

Why We Are Second To None

Meridian offers diagnostic manufacturers the complete solution with over 3,000 antigens and antibodies covering more than 500 diseases and an extensive range of specialty qPCR/RT-qPCR master mixes and molecular enzymes all manufactured under ISO13485 regulations.

  • Path Copy

    Large Sample Size

  • Lot Reservation

    Lot Reservation

  • ISO13485

    ISO13485

  • World-Class Customer Service

    World-Class Customer Service

Get In Touch With A Specialist

Have questions about a product? Want to learn more about Meridian’s molecular or immunoassay reagent portfolio? We want to hear from you!


By submitting your information in this form, you agree that your personal information may be stored and processed in any country where we have facilities or service providers, and by using our “Contact Us” page you agree to the transfer of information to countries outside of your country of residence, including to the United States, which may provide for different data protection rules than in your country. The information you submit will be governed by our Privacy Statement.

Learn More About Us

About
Welcome to
logo blue

Please select your country to continue

Continue
CN