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Uracil DNA Glycosylase

1-Step qPCR Buffer, 4x is a novel buffer system that has been designed for highly reproducible, accurate reverse transcription followed by qPCR in a single tube under fast thermal cycling conditions, delivering excellent results in fast RT-qPCR assays.

*Not available for sale in the United States

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Uracil DNA Glycosylase, MDX054

Available in 10 mL (10,000 Rxns) aliquots

Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases). UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation, and is inhibited by high ionic strength (>200 mM). Uracil DNA Glycosylase is tested for purity (run on SDS-PAGE gel), presence and absence of endonucleases, nickases, exonucleases, and RNases.

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