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DNA/RNA提取质控

Positive controls for IVD molecular assays are available in a range of formats and can be used to assess different steps within an MDx assay process (e.g. extraction, reagent performance, PCR cycling). In general, control material that most closely imitates a real target, in all features, will give the most complete validation. One of the more commonly used controls in qPCR/RT-qPCR assay for pathogen detection are extraction controls that are used in parallel to test specimens. Traditionally these have been variations of the target, for example, chemically inactivated virus or bacteria. However new artificial forms, such as assay-specific target nucleic acids artificially encapsulated in something similar to a live organism (virus like particle (VLP)), are frequently sought out due to their easiness to handle and stability and the ability to customize the sequence being used.

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姓名 Specimen Type Application   Order a Sample Sizes Description Wet(Liquid) Air-Dryable Dryable Glycerol-Free Lyo-Ready Concentration High Concentration Detail Safety Data Sheet COA/Test Release 使用手册 产品指南
VLP-RNA Extraction Control Red VLP Extraction Controls RT-qPCR MDX068 Order a Sample Available in 1 mL (~1×10^4 copies/µL) and 20 mL (~1×10^4 copies/µL) aliquots

An Internal Control RNA sequence, with no known homology to any organism, encapsidated in a virus-like particle and a VLP Detection Mix containing primers and probe to the internal control RNA sequence.

Yes Yes Air-Dryable,Lyo-Ready YesYes Yes 10^4 copies/µL No
VLP-RNA Extraction Control Orange VLP Extraction Controls RT-qPCR MDX069 Order a Sample Available in 1 mL (~1×10^4 copies/µL) and 20 mL (~1×10^4 copies/µL) aliquots

An Internal Control RNA sequence, with no known homology to any organism, encapsidated in a virus-like particle and a VLP Detection Mix containing primers and probe to the internal control RNA sequence.

Yes Yes Air-Dryable,Lyo-Ready YesYes Yes 10^4 copies/µL No
VLP-RNA Extraction Control VLP Extraction Controls RT-qPCR MDX071 Order a Sample Custom

An internal custom control RNA sequence, encapsidated in a virus-like particle.

Yes Yes Air-Dryable,Lyo-Ready YesYes Yes Custom No
qPCR Extraction Control Red Extraction Controls qPCR MDX026 Order a Sample Available in 10 mL (2,000 Rxns) aliquots

Control used to monitor the success of a qPCR assay from lysis/extraction to amplification and reduces the chance of obtaining false negative results from the sample DNA.

Yes No No NoNo No 25x No
qPCR Extraction Control Orange Extraction Controls qPCR MDX027 Order a Sample Available in 10 mL (2,000 Rxns) aliquots

Control used to monitor the success of a qPCR assay from lysis/extraction to amplification and reduces the chance of obtaining false negative results from the sample DNA.

Yes No No NoNo No 25x No
RT-qPCR Extraction Control Red Extraction Controls RT-qPCR MDX028 Order a Sample Available in 1 mL (500 Rxns) aliquots

Control used to monitor the success of an RT-qPCR assay from lysis/extraction to amplification and reduces the chance of obtaining false negative results from the sample RNA.

Yes No No NoNo No 25x No
RT-qPCR Extraction Control Orange Extraction Controls RT-qPCR MDX029 Order a Sample Available in 1 mL (500 Rxns) aliquots

Control used to monitor the success of an RT-qPCR assay from lysis/extraction to amplification and reduces the chance of obtaining false negative results from the sample RNA.

Yes No No NoNo No 25x No

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