等温扩增
以环介导等温扩增LAMP为例,它使用4到6个引物来识别目标DNA中的几个特定区域。 该反应由链置换聚合酶(如Bst DNA聚合酶)和两个特别设计的引物启动,形成环状结构,然后进行后续快速大量的DNA扩增。 LAMP反应对样本中的抑制物有较强耐受性能,能在短时间内产生大量的DNA,可以通过裸眼观察(如沉淀或颜色变化)。 具体来说,1 ~ 10个拷贝DNA可在30分钟内扩增到10^9 ~ 10^10个拷贝,9 to 1010 具有高灵敏度和特异性。
迈迪安还开发了特定样本类型的抗抑性LAMP预混液,设计用于从粗裂解液或富含抑制剂样品的直接扩增,具有更强的抑制物耐受性能。这些预混液可以用于液体体系,也可以通过冻干或风干开发常温稳定的检测用于室温运输和存储,延长保质期,并增加加样量提高样本灵敏度,是开发POCT快速检测的理想选择。
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Products (20)
姓名 | Specimen Type | Application | Order a Sample | Sizes | Description | Wet(Liquid) | Air-Dryable | Dryable | Glycerol-Free | Lyo-Ready | Concentration | High Concentration | Detail | Safety Data Sheet | COA/Test Release | 使用手册 | 产品指南 | |||
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Glycerol-Free RNase H (HC) | Reverse Transcriptase, NGS Enzymes, NASBA | RT-qPCR | MDX202 | Order a Sample | Available in 100 µL (2,500 Unit) or 250 µL (12,500 Unit) aliquots | Glycerol-free, high concentration RNA-specific endonuclease that hydrolyzes the phosphodiester bonds of RNA, when hybridized to DNA. Ideal for lyophilization. |
Yes | Requires excipients | Requires excipients | YesYes | Requires excipients | 50 U/µL | Yes | View Details | COA/Test Release | 产品指南 | ||||
Glycerol-Free T7 RNA Polymerase (HC) | NASBA | NASBA | MDX201 | Order a Sample | Available in 50,000 Unit or 500,000 Unit aliquots | Glycerol-free, high concentration DNA-dependant RNA Polymerase that requires a T7 promoter sequence, it an ideal enzyme for in-vitro synthesis of labeled or unlabeled RNA for research, diagnostic or clinical applications. Ideal for lyophilization. |
Yes | Requires excipients | Requires excipients | YesYes | Requires excipients | 1,000 U/µL | Yes | View Details | COA/Test Release | 产品指南 | ||||
Lyo-Ready Direct RNA/DNA LAMP Urine, 4x | Urine, LAMP Mixes | RT-LAMP | MDX155 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer. |
Yes | No | Lyo-Ready | YesYes | Yes | 4x | Yes | View Details | COA/Test Release | 使用手册 | 产品指南 | |||
Lyo-Ready Direct DNA LAMP Urine, 4x | Urine, LAMP Mixes | LAMP | MDX154 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer. |
Yes | No | Lyo-Ready | YesYes | Yes | 4x | Yes | View Details | COA/Test Release | 使用手册 | 产品指南 | |||
Lyo-Ready Direct DNA LAMP Blood, 4x | Blood, Plasma, Serum, LAMP Mixes | LAMP | MDX124 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 20% whole blood, 20% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications. |
Yes | No | Lyo-Ready | YesYes | Yes | 4x | Yes | View Details | Safety Data Sheet | COA/Test Release | 使用手册 | 产品指南 | ||
Lyo-Ready Direct RNA/DNA LAMP Stool, 4x | Stool, LAMP Mixes | RT-LAMP | MDX145 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 5% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses. |
Yes | No | Lyo-Ready | YesYes | Yes | 4x | Yes | View Details | Safety Data Sheet | COA/Test Release | 使用手册 | 产品指南 | ||
Lyo-Ready Direct DNA LAMP Stool, 4x | Stool, LAMP Mixes | LAMP | MDX144 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 20% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses. |
Yes | No | Lyo-Ready | YesYes | Yes | 4x | Yes | View Details | Safety Data Sheet | COA/Test Release | 使用手册 | 产品指南 | ||
Lyo-Ready Direct RNA/DNA LAMP Saliva, 4x | Saliva, Sputum, LAMP Mixes | RT-LAMP | MDX135 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections. |
Yes | No | Lyo-Ready | YesYes | Yes | 4x | Yes | View Details | Safety Data Sheet | COA/Test Release | 使用手册 | 产品指南 | ||
Lyo-Ready Direct DNA LAMP Saliva, 4x | Saliva, Sputum, LAMP Mixes | LAMP | MDX134 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections. |
Yes | No | Lyo-Ready | YesYes | Yes | 4x | Yes | View Details | Safety Data Sheet | COA/Test Release | 使用手册 | 产品指南 | ||
Lyo-Ready Direct RNA/DNA LAMP Blood, 4x | Blood, Plasma, Serum, LAMP Mixes | RT-LAMP | MDX125 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 10% whole blood, 10% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications. |
Yes | No | Lyo-Ready | YesYes | Yes | 4x | Yes | View Details | COA/Test Release | 使用手册 | 产品指南 | |||
Air-Dryable DNA LAMP | LAMP Mixes | LAMP | MDX119 | Order a Sample | Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots | Glycerol-free mix for isothermal applications such as LAMP, containing Bst DNA polymerase, reaction buffer, dNTPs and air-dryable excipients, ideal for developing ambient temperature, LAMP assays. |
Yes | Yes | Air-Dryable | YesYes | No | 4x | Yes | View Details | COA/Test Release | 使用手册 | 产品指南 | |||
Air-Dryable RT-LAMP 1-Step Mix, 4x | LAMP Mixes | RT-LAMP | MDX118 | Order a Sample | Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots | Glycerol-free mix for isothermal applications such as RT-LAMP, containing Bst DNA polymerase, reverse transcriptase, reaction buffer, dNTPs and air-dryable excipients, ideal for developing ambient temperature, LAMP assays. |
Yes | Yes | Air-Dryable | YesYes | No | 4x | Yes | View Details | Safety Data Sheet | COA/Test Release | 使用手册 | 产品指南 | ||
Lyo-Ready RT-LAMP 1-Step Mix, 4x | LAMP Mixes | RT-LAMP | MDX108 | Order a Sample | Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots | Glycerol-free mix for isothermal applications such as RT-LAMP, containing Bst DNA polymerase, reverse transcriptase, reaction buffer, dNTPs and lyo-compatible excipients, ideal for developing ambient temperature, LAMP assays. |
Yes | No | Lyo-Ready | YesYes | Yes | 4x | Yes | View Details | COA/Test Release | 使用手册 | 产品指南 | |||
Lyo-Ready LAMP Mix, 4x | LAMP Mixes | LAMP | MDX097 | Order a Sample | Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots | Glycerol-free mix for isothermal applications such as LAMP, containing Bst DNA polymerase, reaction buffer, dNTPs and lyo-compatible excipients, ideal for developing ambient temperature, LAMP assays. |
Yes | No | Lyo-Ready | YesYes | Yes | 4x | Yes | View Details | COA/Test Release | 使用手册 | 产品指南 | |||
Enzyme Dilution Buffer, 10x (Glycerol free) | Buffer, Bst Enzymes & Buffers | PCR | MDX080 | Order a Sample | Available in 5 mL or 100 mL aliquots | Glycerol-free,10x dilution buffer, for the dilution of enzymes to reaction concentration. |
Yes | Yes | Air-Dryable,Lyo-Ready | YesYes | Yes | 10x | No | View Details | Safety Data Sheet | COA/Test Release | 产品指南 | |||
Enzyme Dilution Buffer, 1x | Buffer, Bst Enzymes & Buffers | PCR | MDX078 | Order a Sample | Available in 5 mL or 100 mL aliquots | Glycerol containing1x dilution buffer, for the dilution of enzymes to reaction concentration. |
Yes | No | No | NoNo | No | 1x | No | View Details | Safety Data Sheet | COA/Test Release | 产品指南 | |||
Bst Reaction Buffer, 10x | Buffer, Bst Enzymes & Buffers | LAMP | MDX076 | Order a Sample | Available in 5 mL or 100 mL aliquots | Reaction Buffer optimized for use with Bst DNA Polymerase. |
Yes | Requires excipients | Requires excipients | YesYes | Requires excipients | 10x | No | View Details | Safety Data Sheet | COA/Test Release | 产品指南 | |||
Bst DNA Polymerase | Isothermal DNA Polymerase, Bst Enzymes & Buffers | LAMP | MDX012 | Order a Sample | Available in 1 mL or 10 mL aliquots | DNA polymerase (exo-), with strand-displacement properties. used for Isothermal DNA amplification such as LAMP (Loop-mediated Isothermal Amplification). |
Yes | No | No | NoNo | No | 8 units/mL | No | View Details | Safety Data Sheet | COA/Test Release | 产品指南 | |||
Inhibitor-Tolerant Bst Buffer | Buffer, Bst Enzymes & Buffers | LAMP | MDX019 | Order a Sample | Available in 2 mL or 100 mL aliquots | Inhibitor-Tolerant Bst Buffer is specially designed to overcome the inhibition present in samples in direct LAMP reactions. |
Yes | Requires excipients | Requires excipients | YesYes | Requires excipients | 10x | No | View Details | Safety Data Sheet | COA/Test Release | 产品指南 | |||
High Conc. Glycerol-Free Bst | Isothermal DNA Polymerase, Bst Enzymes & Buffers | LAMP | MDX018 | Order a Sample | Available in 80 µL or 800 µL aliquots | High concentration glycerol-free DNA polymerase (exo-), with strand-displacement properties. used for Isothermal DNA amplification such as LAMP (Loop-mediated Isothermal Amplification). |
Yes | Requires excipients | Requires excipients | YesYes | Requires excipients | 100 units/mL | Yes | View Details | Safety Data Sheet | COA/Test Release | 产品指南 |
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产品资料
Meridian Air Dryable Direct Stool可冻干LAMP试剂
Meridian Air Dryable Direct Stool可冻干RT-LAMP试剂
Meridian Air Dryable Direct StoolBst DNA Polymerase
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