等温扩增

等温扩增(包括HAD、MCA和LAMP)是一种在即时诊断(POC)中发挥着越来越重要作用的DNA扩增技术。与qPCR和其他扩增技术相比，它具有快速、灵敏、低成本、方便等方面的优势，是在实验资源有限地区进行即时检测的理想选择。等温扩增已被广泛应用于病原体检测（如沙门氏菌和疟疾），转基因作物污染，以及法医检测等。

以环介导等温扩增LAMP为例，它使用4到6个引物来识别目标DNA中的几个特定区域。该反应由链置换聚合酶(如Bst DNA聚合酶)和两个特别设计的引物启动，形成环状结构，然后进行后续快速大量的DNA扩增。 LAMP反应对样本中的抑制物有较强耐受性能，能在短时间内产生大量的DNA，可以通过裸眼观察(如沉淀或颜色变化)。具体来说，1 ~ 10个拷贝DNA可在30分钟内扩增到10^9 ~ 10^10个拷贝，⁹ to 10¹⁰ 具有高灵敏度和特异性。

迈迪安还开发了特定样本类型的抗抑性LAMP预混液，设计用于从粗裂解液或富含抑制剂样品的直接扩增，具有更强的抑制物耐受性能。这些预混液可以用于液体体系，也可以通过冻干或风干开发常温稳定的检测用于室温运输和存储，延长保质期，并增加加样量提高样本灵敏度，是开发POCT快速检测的理想选择。

Meridian搜索

使用迈迪安智能搜索，在下方输入疾病、产品名称或产品编号查找您需要的产品：

Products (20)

Filters Close

姓名	Specimen Type	Application		Order a Sample	Sizes	Description	Wet(Liquid)	Air-Dryable	Dryable	Glycerol-Free	Lyo-Ready	Concentration	High Concentration	Detail	Safety Data Sheet	COA/Test Release	使用手册	产品指南
Glycerol-Free RNase H (HC)	Reverse Transcriptase, NGS Enzymes, NASBA	RT-qPCR	MDX202	Order a Sample	Available in 100 µL (2,500 Unit) or 250 µL (12,500 Unit) aliquots	Glycerol-free, high concentration RNA-specific endonuclease that hydrolyzes the phosphodiester bonds of RNA, when hybridized to DNA. Ideal for lyophilization.	Yes	Requires excipients	Requires excipients	YesYes	Requires excipients	50 U/µL	Yes	View Details		COA/Test Release		产品指南
Glycerol-Free T7 RNA Polymerase (HC)	NASBA	NASBA	MDX201	Order a Sample	Available in 50,000 Unit or 500,000 Unit aliquots	Glycerol-free, high concentration DNA-dependant RNA Polymerase that requires a T7 promoter sequence, it an ideal enzyme for in-vitro synthesis of labeled or unlabeled RNA for research, diagnostic or clinical applications. Ideal for lyophilization.	Yes	Requires excipients	Requires excipients	YesYes	Requires excipients	1,000 U/µL	Yes	View Details		COA/Test Release		产品指南
Lyo-Ready Direct RNA/DNA LAMP Urine, 4x	Urine, LAMP Mixes	RT-LAMP	MDX155	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.	Yes	No	Lyo-Ready	YesYes	Yes	4x	Yes	View Details		COA/Test Release	使用手册	产品指南
Lyo-Ready Direct DNA LAMP Urine, 4x	Urine, LAMP Mixes	LAMP	MDX154	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.	Yes	No	Lyo-Ready	YesYes	Yes	4x	Yes	View Details		COA/Test Release	使用手册	产品指南
Lyo-Ready Direct DNA LAMP Blood, 4x	Blood, Plasma, Serum, LAMP Mixes	LAMP	MDX124	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 20% whole blood, 20% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.	Yes	No	Lyo-Ready	YesYes	Yes	4x	Yes	View Details	Safety Data Sheet	COA/Test Release	使用手册	产品指南
Lyo-Ready Direct RNA/DNA LAMP Stool, 4x	Stool, LAMP Mixes	RT-LAMP	MDX145	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 5% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.	Yes	No	Lyo-Ready	YesYes	Yes	4x	Yes	View Details	Safety Data Sheet	COA/Test Release	使用手册	产品指南
Lyo-Ready Direct DNA LAMP Stool, 4x	Stool, LAMP Mixes	LAMP	MDX144	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 20% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.	Yes	No	Lyo-Ready	YesYes	Yes	4x	Yes	View Details	Safety Data Sheet	COA/Test Release	使用手册	产品指南
Lyo-Ready Direct RNA/DNA LAMP Saliva, 4x	Saliva, Sputum, LAMP Mixes	RT-LAMP	MDX135	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.	Yes	No	Lyo-Ready	YesYes	Yes	4x	Yes	View Details	Safety Data Sheet	COA/Test Release	使用手册	产品指南
Lyo-Ready Direct DNA LAMP Saliva, 4x	Saliva, Sputum, LAMP Mixes	LAMP	MDX134	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.	Yes	No	Lyo-Ready	YesYes	Yes	4x	Yes	View Details	Safety Data Sheet	COA/Test Release	使用手册	产品指南
Lyo-Ready Direct RNA/DNA LAMP Blood, 4x	Blood, Plasma, Serum, LAMP Mixes	RT-LAMP	MDX125	Order a Sample	Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots	Inhibitor tolerance to 10% whole blood, 10% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.	Yes	No	Lyo-Ready	YesYes	Yes	4x	Yes	View Details		COA/Test Release	使用手册	产品指南
Air-Dryable DNA LAMP	LAMP Mixes	LAMP	MDX119	Order a Sample	Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots	Glycerol-free mix for isothermal applications such as LAMP, containing Bst DNA polymerase, reaction buffer, dNTPs and air-dryable excipients, ideal for developing ambient temperature, LAMP assays.	Yes	Yes	Air-Dryable	YesYes	No	4x	Yes	View Details		COA/Test Release	使用手册	产品指南
Air-Dryable RT-LAMP 1-Step Mix, 4x	LAMP Mixes	RT-LAMP	MDX118	Order a Sample	Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots	Glycerol-free mix for isothermal applications such as RT-LAMP, containing Bst DNA polymerase, reverse transcriptase, reaction buffer, dNTPs and air-dryable excipients, ideal for developing ambient temperature, LAMP assays.	Yes	Yes	Air-Dryable	YesYes	No	4x	Yes	View Details	Safety Data Sheet	COA/Test Release	使用手册	产品指南
Lyo-Ready RT-LAMP 1-Step Mix, 4x	LAMP Mixes	RT-LAMP	MDX108	Order a Sample	Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots	Glycerol-free mix for isothermal applications such as RT-LAMP, containing Bst DNA polymerase, reverse transcriptase, reaction buffer, dNTPs and lyo-compatible excipients, ideal for developing ambient temperature, LAMP assays.	Yes	No	Lyo-Ready	YesYes	Yes	4x	Yes	View Details		COA/Test Release	使用手册	产品指南
Lyo-Ready LAMP Mix, 4x	LAMP Mixes	LAMP	MDX097	Order a Sample	Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots	Glycerol-free mix for isothermal applications such as LAMP, containing Bst DNA polymerase, reaction buffer, dNTPs and lyo-compatible excipients, ideal for developing ambient temperature, LAMP assays.	Yes	No	Lyo-Ready	YesYes	Yes	4x	Yes	View Details		COA/Test Release	使用手册	产品指南
Enzyme Dilution Buffer, 10x (Glycerol free)	Buffer, Bst Enzymes & Buffers	PCR	MDX080	Order a Sample	Available in 5 mL or 100 mL aliquots	Glycerol-free,10x dilution buffer, for the dilution of enzymes to reaction concentration.	Yes	Yes	Air-Dryable,Lyo-Ready	YesYes	Yes	10x	No	View Details	Safety Data Sheet	COA/Test Release		产品指南
Enzyme Dilution Buffer, 1x	Buffer, Bst Enzymes & Buffers	PCR	MDX078	Order a Sample	Available in 5 mL or 100 mL aliquots	Glycerol containing1x dilution buffer, for the dilution of enzymes to reaction concentration.	Yes	No	No	NoNo	No	1x	No	View Details	Safety Data Sheet	COA/Test Release		产品指南
Bst Reaction Buffer, 10x	Buffer, Bst Enzymes & Buffers	LAMP	MDX076	Order a Sample	Available in 5 mL or 100 mL aliquots	Reaction Buffer optimized for use with Bst DNA Polymerase.	Yes	Requires excipients	Requires excipients	YesYes	Requires excipients	10x	No	View Details	Safety Data Sheet	COA/Test Release		产品指南
Bst DNA Polymerase	Isothermal DNA Polymerase, Bst Enzymes & Buffers	LAMP	MDX012	Order a Sample	Available in 1 mL or 10 mL aliquots	DNA polymerase (exo-), with strand-displacement properties. used for Isothermal DNA amplification such as LAMP (Loop-mediated Isothermal Amplification).	Yes	No	No	NoNo	No	8 units/mL	No	View Details	Safety Data Sheet	COA/Test Release		产品指南
Inhibitor-Tolerant Bst Buffer	Buffer, Bst Enzymes & Buffers	LAMP	MDX019	Order a Sample	Available in 2 mL or 100 mL aliquots	Inhibitor-Tolerant Bst Buffer is specially designed to overcome the inhibition present in samples in direct LAMP reactions.	Yes	Requires excipients	Requires excipients	YesYes	Requires excipients	10x	No	View Details	Safety Data Sheet	COA/Test Release		产品指南
High Conc. Glycerol-Free Bst	Isothermal DNA Polymerase, Bst Enzymes & Buffers	LAMP	MDX018	Order a Sample	Available in 80 µL or 800 µL aliquots	High concentration glycerol-free DNA polymerase (exo-), with strand-displacement properties. used for Isothermal DNA amplification such as LAMP (Loop-mediated Isothermal Amplification).	Yes	Requires excipients	Requires excipients	YesYes	Requires excipients	100 units/mL	Yes	View Details	Safety Data Sheet	COA/Test Release		产品指南



Specimen Type
Application

Sizes
Wet(Liquid)
Air-Dryable
Glycerol-Free
Lyo-Ready
Concentration
High Concentration
Description





点击此处订购样品

找不到您想要的产品？联系我们咨询

等温扩增

Meridian搜索

Product Filters

Specimen Type

Application

Dryable

High Concentration

Products (20)