Bst Enzymes & Buffers
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Faster Polymerization, and Enhanced Tolerance in Point of Care Testing
- Glycerol-free options, to eliminate the need for cold-chain shipping and storage.
- High-concentrated, to maximize sample input and increase sensitivity.
- Fast reaction kinetics for quicker time to results.
Bst DNA Polymerase enzyme is supplied at standard concentration (8 U/µL), or as a high-concentration, glycerol-free enzyme (100 U/µL) and is used with an Enzyme Dilution Buffer and a Bst Reaction Buffer, formulated to deliver the fastest amplification speed (time-to-results), yield, salt tolerance, and sensitivity, speeding up your product development time and allowing you to commercialize your new products faster. The high-concentration, glycerol-free enzyme is also lyophilization-compatible, making it ideal for the development of point of care test (POCT) assays that require ambient temperature shipping and storage.
产品资料
Bst DNA聚合酶 Bst DNA聚合酶
可冻干LAMP试剂 可冻干LAMP试剂
可冻干RT-LAMP试剂 可冻干RT-LAMP试剂
可风干LAMP/RT-LAMP 试剂 可风干LAMP/RT-LAMP 试剂
环介导等温扩增与qPCR的主要区别在于，在环介导等温扩增中，扩增是在恒温下完成的，而在qPCR中，扩增需要在一个热循环器上在多个温度之间循环。 环介导等温扩增使用具有高链置换活性的聚合酶，如Bst DNA聚合酶，由于LAMP反应不需要通过多个温度的变化，因此可以在短时间内完成快速的扩增。
No Meridian’s Bst DNA polymerase does not come with warm or hot start; instead, the reaction buffer does not contain magnesium and so the enzyme is completely inactive until magnesium is added. If you are concerned about setting your reaction up at room temperature, we recommend adding the magnesium last, just before running the experiment and it will prevent non-specific amplification.
Yes, it is possible to design a multiplex LAMP test using different primers and probes, however they can be difficult to design and optimize as each sequence requires three sets of primers.
Can Bst DNA Polymerase be used in place of other DNA polymerases for thermal cycling?
No, the reaction temperatures are too high for enzyme stability.
Yes, Bst DNA polymerase does have some reverse transcriptase activity, however for robust and consistent reactions we recommend the addition of a reverse transcriptase.
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