可风干™ Direct DNA LAMP Blood Master Mix
可风干™ Direct DNA LAMP Blood mix is a ready-to-use, 4x concentrated, fully optimized mix for isothermal amplification methods such as loop-mediated isothermal amplification (LAMP) on blood. It is designed to dramatically speed up assay development time and reduce time to results. The glycerol-free mix contain optimized excipients compatible with air drying, making it ideal for POC blood testing.
Can also be used wet and/or with purified nucleic acid
Higher Specificity and Quicker Time to Detect Viral Targets
(A) Air-Dryable™ Direct DNA LAMP Blood (MDX126, red) was air-dried with primers for Epstein-Barr virus (EBV), Hepatitis B Virus (HBV), Herpes Simplex Virus 2 (HSV2), Trichomonas vaginalis and Mycoplasma pneumoniae. The performance of the mix was compared against NEB WarmStart® LAMP kit (orange) and Thermo SuperScript™ IV RT-LAMP Master Mix (black) using the same primers and DNA. Reactions were incubated at 65°C for 60 minutes. Error bars represent the standard deviation across three technical replicates. The Air-Dryable™ Direct LAMP Blood mix delivered faster target amplification (TTR) with higher specificity, as demonstrated by the longest delay in no-template control (NTC) amplification across range of pathogenic DNA targets.
Air-Dryable Direct DNA LAMP Blood, 4x, MDX126
对 10% 全血和 10% 血浆具有抑制剂耐受性，与所有抗凝剂兼容。非常适合多重检测和快速循环条件。用于检测血液病原体和液体活检应用。
文档与资源
Blood is one of the most common specimens used for laboratory diagnostic testing and it is useful for diagnosing diseases such as bacterial and viral infections, cancer, cardiovascular disease, and metabolic disorders such as diabetes. However, whole blood specimens, serum, and plasma contain a number of inherent PCR inhibitors including immunoglobulin G, hemoglobin, lactoferrin, and leukocyte DNA. In addition, PCR inhibitors can be found in the anticoagulants used to stabilize blood samples (e.g. EDTA, citrate, or heparin). Isothermal amplification such as LAMP is very tolerant to inhibitors and so is ideal for point of care (POC) testing, the simple workflow allows fast, sensitive and reliable results in clinical diagnosis.
The Air-Dryable Direct DNA LAMP Blood mix combines even greater tolerance with fast, robust isothermal amplification to enable highly sensitive direct detection using crudely processed whole blood, plasma, or serum samples. The mix are uniquely designed to overcome much higher concentrations of inhibitors, and so do not require a DNA purification step to concentrate and purify a patient sample. Very fast reaction kinetics result in faster time to results and superior amplification sensitivity and specificity. In addition, the 4x concentrated master mixes are formulated with excipients which are optimized for air drying to create ambient-temperature stable assays, making them ideal for POC blood testing.
产品资料
可冻干血液直扩LAMP预混液可冻干血液直扩LAMP预混液
可冻干LAMP试剂可冻干LAMP试剂
可冻干RT-LAMP试剂可冻干RT-LAMP试剂
可风干LAMP/RT-LAMP 试剂可风干LAMP/RT-LAMP 试剂
FAQs: LAMP Saliva Testing
环介导等温扩增与qPCR的主要区别在于，在环介导等温扩增中，扩增是在恒温下完成的，而在qPCR中，扩增需要在一个热循环器上在多个温度之间循环。 环介导等温扩增使用具有高链置换活性的聚合酶，如Bst DNA聚合酶，由于LAMP反应不需要通过多个温度的变化，因此可以在短时间内完成快速的扩增。
Blood contains abundant information about the health status of the organism, it can be used to evaluate organ function, detect proteins, nucleic acids, metabolites, infectious diseases and can be used for diagnosis of cancer (liquid biopsy), heart disease, diabetes. For POC Blood testing it is a simple and easily accessible (low-invasive) method to for monitor health.
Immunoglobulin G is the major inhibitor of LAMP in blood, as it can bind to single-stranded genomic DNA, reducing the sensitivity of the whole blood test. The other two main inhibitors in blood are hemoglobin and lactoferrin that affected the Bst DNA polymerase activity and thus lowered the amplification efficiency. Hemoglobin and hematin can also cause some fluorescence quenching, particularly for FAM, although this should not affect the time to results (TTR). In addition, inhibitors can be found in the anticoagulants used to stabilize blood samples (e.g. EDTA, citrate, or heparin).
We have used up to 20% whole blood with our mix, higher concentrations cause the solution to become viscous and difficult to work with.
冻干后的预混液在正确封存的情况下可在常温下稳定保持2年。
Yes, it is possible that you may need to pre-treat the blood in order to release the DNA from the gram-positive bacteria. In some cases, a heat shock is sufficient, this will depend on the bacteria being examined.
Yes, it is possible to create a multiplex LAMP whole blood test using different primers and probes, however they can be difficult to design and optimize as each sequence requires three sets of primers.
They are compatible with standard isothermal amplification protocols; however, some optimization may be required for the amount of blood plasma or serum samples that is needed for the blood tests.
You should elute in water, or TE buffer.
Yes, the Air-Dryable Direct DNA LAMP Blood can be used as a liquid mix or dried and stored for up to 24 months without needing to change the reaction conditions and drying/ambient temperature storage will not affect the sensitivity of the test.
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