Glycerol-Free Thermostable DNA Helicase (HC)

Description

Glycerol-Free Thermostable DNA Helicase (MDX242) is a highly stable, multi-domain enzyme derived from the Thermoanaerobacter tengcongensis uvrD gene (Tte UvrD helicase). It unwinds double-stranded
DNA with 3’ to 5’ polarity using ATP hydrolysis without requiring an overhang structure. Capable of generating single-stranded templates without thermocycling, it remains functional at temperatures up to 65°C.

Key points:

• Glycerol-Free Thermostable DNA Helicase generates single-stranded templates without thermocycling (resistant up to 60-65°C)
• Ideal for robust, efficient, and precise DNA amplification
• Broader applications include NGS library preparation, SNP detection, mutation analysis, and coupling with CRISPR-based systems to enhance DNA accessibility for Cas enzyme binding

Glycerol-Free Thermostable DNA Helicase (HC) is fast

^{Glycerol-Free Thermostable DNA Helicase (HC) was used in a DNA unwinding assay. Glycerol-Free Thermostable DNA Helicase (HC) was used at 1 ng/μL with 50 nM of double-stranded DNA substrate in Lyo-Ready Thermostable Helicase Reaction Buffer, compared to another supplier under the same conditions. The results demonstrate that Meridian’s Glycerol-Free Thermostable DNA Helicase (HC) has a higher reaction rate than other suppliers.}