Air-Dryable & Lyo-Ready Enzymes
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High-Sensitivity Air Dryable and Lyo Ready Enzymes for Assays
- Glycerol-free solution, for preparation of dried-down assays.
- High concentration, allowing smaller reaction volumes and greater flexibility in assay optimization.
- Ideal for POC microfluidic devices.
Meridian high-concentration, glycerol-free enzymes help you develop highly sensitive assays. They have been developed for speed and are designed for superior sensitivity and yield. The high concentration Being glycerol-free allows for the option of lyophilization or air-drying, allowing ambient temperature shipping and storage, and increased flexibility in sample volume.
- Glycerol-Free Taq HS 50U/µL
- Glycerol-Free Taq HS High Conc.
- Aptamer Taq HS (Glycerol-Free)
- Glycerol-Free High-Fidelity Pfu (HC)
- Glycerol-Free HS Tth DNA Polymerase (HC)
- High Conc. Glycerol-Free Bst
- Glycerol-Free T7 RNA Polymerase (HC)
- 55C MMLV-RT
- Lyo-Compatible MMLV-RT
- RNase Inhibitor (Glycerol Free)
- Glycerol-Free RNase H (HC)
- Glycerol-free T4 DNA Ligase (HC)
产品资料
无甘油 Taq HS聚合酶无甘油 Taq HS聚合酶
Aptamer Taq HS (Glycerol-Free)Aptamer Taq HS (Glycerol-Free)
Pfu高保真热启动聚合酶Pfu高保真热启动聚合酶
Glycerol-Free Tth DNA Polymerase (HC)Glycerol-Free Tth DNA Polymerase (HC)
无甘油
T7 RNA 聚合酶无甘油
T7 RNA 聚合酶
55C MMLV-RT55C MMLV-RT
无甘油 T4 DNA 连接酶（HC）无甘油 T4 DNA 连接酶（HC）
冻干是在真空中冷冻和升华，形成饼状或珠状，粘度非常低，可去除高达99%的水分。干燥通常需要 18-24 小时，并需要在冷冻过程中保护酶的低温赋形剂。试剂可冻干成珠状或饼状。
风干是在对流烘箱中加热，形成高粘度颗粒，去除约 95% 的水分。干燥过程通常不超过 2 小时，并且需要在较高温度下保护酶的热保护辅料。试剂可以风干成软粘稠状。
冻干和风干有助于延长产品的保质期，提高试剂在较高温度下的稳定性，并能方便、准确地分配试剂。干燥后，试剂很容易与样品材料重组。
Yes, reaction conditions are the same and they will produce the same results, this means that the liquid mixes can be used to create SOPs if you do not want to dry down and then later if drying is is required (for example to increase sensitivity), the SOP can be updated for drying, it does not require completely new SOPs to be written, but excipients will need to be added.
Following drying in the presence or absence of a buffer containing excipients, primers/probes, the mix should be stable for a minimum of 24 months at ambient temperature. Following rehydration, the assay reproducibility, sensitivity, and robustness should be the same as for a freshly made liquid mix.
No, our lyo-excipients are proprietary, if you require a buffer that contains lyo-excipients, we have the Lyo-Ready qPCR Buffer, 2.5x (MDX022).
高浓度的酶有助于在干燥时减少体积，使过程更快，它们还可以减少微流控、卡壳和POC设备的反应体积，从而可以添加更多的样品。它们有助于优化并可以加快反应（只要有模板可结合），提高灵敏度和产率，因此，可以用于广泛的测定形式，从而在商业化过程中实现更大的开发灵活性和成本节约。
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