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DNA RNA Extraction Controls

DNA/RNA提取质控

Meridian’s DNA Extraction Controls use 大肠杆菌 cells and RNA Extraction Controls use artificial micelles, of a known concentration that contain quantitative PCR positive control DNA or RNA sequence (with no known homology to any organism, so that it does not interfere with the detection of the sample target sequence). Their composition closely resembles a test sample and serves as a full-process PCR positive control, monitoring the success of an assay from lysis/extraction to amplification.
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名称 详细信息 应用 目录 订购样品 尺寸 说明 湿（液体） 可风干 名称 不含甘油 Lyo-Ready 浓度 高浓度 详细信息 安全数据表 COA/Test Release 用户指南 产品处理指南 产品手册
qPCR Extraction Control Red 脱氧核糖核酸 qPCR MDX026 订购样品 Available in 10 mL (2,000 Rxns) aliquots

Control used to monitor the success of a qPCR assay from lysis/extraction to amplification and reduces the chance of obtaining false negative results from the sample DNA.

 液体 没有 没有 没有 没有 25x 没有 查看详情 安全数据表 COA/Test Release 产品指南
qPCR Extraction Control Orange 脱氧核糖核酸 qPCR MDX027 订购样品 Available in 10 mL (2,000 Rxns) aliquots

Control used to monitor the success of a qPCR assay from lysis/extraction to amplification and reduces the chance of obtaining false negative results from the sample DNA.

 液体 没有 没有 没有 没有 25x 没有 查看详情 安全数据表 COA/Test Release 产品指南
RT-qPCR Extraction Control Red 核糖核酸 RT-qPCR MDX028 订购样品 Available in 1 mL (500 Rxns) aliquots

Control used to monitor the success of an RT-qPCR assay from lysis/extraction to amplification and reduces the chance of obtaining false negative results from the sample RNA.

 液体 没有 没有 没有 没有 25x 没有 查看详情 安全数据表 COA/Test Release 产品指南
RT-qPCR Extraction Control Orange 核糖核酸 RT-qPCR MDX029 订购样品 Available in 1 mL (500 Rxns) aliquots

Control used to monitor the success of an RT-qPCR assay from lysis/extraction to amplification and reduces the chance of obtaining false negative results from the sample RNA.

 液体 没有 没有 没有 没有 25x 没有 查看详情 安全数据表 COA/Test Release 产品指南
VLP-RNA Extraction Control Red 核糖核酸 RT-qPCR MDX068 订购样品 Available in 1 mL (~1×10^4 copies/µL) and 20 mL (~1×10^4 copies/µL) aliquots

An Internal Control RNA sequence, with no known homology to any organism, encapsidated in a virus-like particle and a VLP Detection Mix containing primers and probe to the internal control RNA sequence.

 液体 需要辅料 可晾干， Lyo-Ready 是 需要辅料 10^4 copies/µL 没有 查看详情 安全数据表 COA/Test Release 产品指南
VLP-RNA Extraction Control Orange 核糖核酸 RT-qPCR MDX069 订购样品 Available in 1 mL (~1×10^4 copies/µL) and 20 mL (~1×10^4 copies/µL) aliquots

An Internal Control RNA sequence, with no known homology to any organism, encapsidated in a virus-like particle and a VLP Detection Mix containing primers and probe to the internal control RNA sequence.

 液体 需要辅料 可晾干， Lyo-Ready 是 需要辅料 10^4 copies/µL 没有 查看详情 安全数据表 COA/Test Release 产品指南

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Why We Are Second To None

Meridian offers diagnostic manufacturers the complete solution with over 3,000 antigens and antibodies covering more than 500 diseases and an extensive range of specialty qPCR/RT-qPCR master mixes and molecular enzymes all manufactured under ISO13485 regulations.

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  • Extraction control undergoes the same processing as test sample.
  • Confirms successes of extraction step and monitors co-purification of PCR inhibitors.
  • Probe based designed specifically for multiplex qPCR/RT-qPCR assays.

The extraction controls comprise an internal control sequence in use E. coli cells or artificial micelles and a control mix (containing specific primer and probe (emission wavelength = 560nm or 670nm)) for qPCR detection. The cells are added to the sample or lysis buffer and lysed and extracted with the sample for downstream amplification. The detection of extraction control acts as a PCR positive control and confirms the success of the extraction and amplification steps, avoiding the misinterpretation of a PCR false negative result.

FAQ's

The benefit of the artificial Extraction Control in evaluation of the extraction process is the possibility to validate sample extraction process. Signal derived from the Extraction Control confirms the success of the extraction step and monitors co-purification of PCR inhibitors that may cause biased or PCR false negative results. Housekeeping genes or spike in controls do not monitor the whole extraction process, especially the lysis step and in the case of housekeeping genes, extraction efficiency and degraded samples as well as the possibility of the housekeeping gene expression level changing.

The primers and probe for the Extraction Controls are in the Control Mix.

The two colors are for the fluorescent dyes on the Extraction Control probe. Control Mix Red (Cy5 – emission wavelength = 670nm) and Control Mix Orange (HEX – emission wavelength = 560nm). This allows you to choose one that will fit with your existing protocol in a multiplex assay.

No, the Extraction Control will work with commercially available silica-membrane extraction kits and CHELEX matrices and has been tested on a wide range of qPCR platforms.

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