Lyo-Ready Direct DNA and RNA/DNA qPCR Urine Master Mixes

The advantages of a quantitative PCR urine test are that it is completely non-invasive, and it can be self-sampled in large quantities. Urine testing is used in the diagnosis and management of infectious diseases (including STDs), urinary tract infections (UTIs) and bladder cancer.

The most common infections are urinary tract infections (UTIs), most commonly E. coli, P. mirabilis, Enterococcus, Staphylococcus and Klebsiella. This is followed by sexually transmitted diseases (STDs) such as syphilis, gonorrhoea, chlamydia, trichomoniasis, hepatitis B (HBV), herpes simplex virus (HSV), HIV and human papillomavirus (HPV). Several other infections can be diagnosed by urine DNA and urine RNA tests including community-acquired pneumonia (CAP), legionellosis, tuberculosis, congenital cytomegalovirus (CMV) infection, and arboviruses, such as Chikungunya virus, dengue virus and Zika virus. Parasites can also be diagnosed from urine testing for the detection of eggs from Schistosoma haematobium.

Cancer cells or their DNA or RNA are too big to pass through the kidneys and will have to come from the bladder or ureters, so bladder cancer is the most obvious urine cancer, however prostate and cervical cancer are also detectable.

We have used up to 25% human urine with our mix.

冻干后的预混液在正确封存的情况下可在常温下稳定保持2年。

That will depend on the bacteria, for some bacteria you may not need to do any pre-treatment, however some may require heating to 95°C for 5 min and for some tougher bacteria, a Proteinase K step (Proteinase K 55°C for 15 min and then at 98°C for 5 min) or using a non-ionic detergent (such as Tween-20 to final concentration of 0.2% followed by incubation at room temperature for 10 min) may be necessary.

We do not see a performance difference between the two mixes, which is why we call the Lyo-Ready Direct RT-qPCR Urine mix – Lyo-Ready Direct RNA/DNA qPCR Urine mix, as it can be used for both.

Yes, the Lyo-Ready Direct DNA qPCR Urine and Lyo-Ready Direct RNA/DNA qPCR Urine can be used as a liquid mix or lyophilized and stored for up to 24 months without needing to change the reaction conditions and lyophilization/ambient temperature storage will not affect the sensitivity of the test.