High-Specificity Pfu Buffer, 10x
文库扩增是通过PCR进行。 除了增加DNA的数量,PCR扩增还会对两端连接有接头的片段进行富集,因为这些片段将会附着在流动细胞上,并将成为序列。 PCR步骤中不引入图片至关重要,更低的错误率意味着更少的序列覆盖,节省了费用和时间。
Overview
The amplification of a next-generation sequencing (NGS) library is performed by PCR. In addition to increasing the amount of DNA, the PCR amplification step also enriches for fragments that have an adaptor-ligated to each end, as these will be the fragments that attach to the flow cell and will be sequenced. It is important that this PCR step does not introduce mutations or bias to the library, as a lower error rate and more even sequence representation means that less sequence coverage is required, saving expense and time.
Product Specs & Downloads
NGS High-Fidelity Pfu Buffer, 10x, MDX038
Available in 5 mL (1,000 Rxns) aliquots
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