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二代测序

下一代测序 Next Generation Sequencing (NGS) 建库分子酶 requires high-fidelity enzymes for precise and efficient library preparation, amplification, and sequencing. Meridian's glycerol-free NGS enzymes for library preparation provide assay developers with a solution that maximizes sequencing efficiency while ensuring long-term reagent stability. Designed for high-throughput sequencing applications and decentralized POC testing, our enzymes deliver unmatched performance, flexibility, and reliability to advance NGS technologies.

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为什么选择 Meridian?

  • 不含甘油的格式简化了化验冻干过程,延长了室温下的保质期

  • 高浓度可最大限度地增加样品输入量,从而提高灵敏度

  • 量身定制的冻干服务,以满足客户的应用需求,包括珠子格式

  • 利用我们专业团队的专业知识,将您现有的 NGS 酶转化为无甘油配方

  • 定制无甘油酶开发,满足独特的 NGS 工作流程要求

Library mapping efficiency and comparable GC content after lyophilization

Library Average mapped reads (%)
Lyophilized reagents 99.79 ± 0.04
Liquid reagents 99.79 ± 0.12

Library Mapping

Comparison of libraries created using lyophilized and liquid formulations. The table shows mapping efficiency, while the chart displays the GC content of the libraries generated with lyophilized and liquid reagents. In both cases, lyophilized and liquid formulations showed similar results, with good mapping efficiency and comparable GC content.

Product Description & Applications

Meridian’s glycerol-free NGS Library Prep Enzymes enable high-fidelity, high-efficiency library preparation, allowing sequencing kits to be shipped and stored at ambient temperature for greater accessibility. Ambient-temperature NGS assays eliminate cold-chain logistics, expanding access to genomic testing in resource-limited settings. By driving scalable, high-performance sequencing technologies, Meridian is bridging the gap, making NGS-based diagnostics more accessible, efficient, and impactful worldwide.

Next-generation sequencing (NGS) is widely used in clinical diagnostics and precision medicine, with applications spanning oncology, infectious disease, reproductive health, and genetic disorder screening. In cancer care, NGS supports companion diagnostics (CDx) for targeted therapies, minimal residual disease (MRD) monitoring, and liquid biopsy for non-invasive tumor detection. It plays a crucial role in infectious disease diagnostics, identifying pathogens, antimicrobial resistance markers, and tracking viral outbreaks. In reproductive medicine, NGS enables non-invasive prenatal testing (NIPT), carrier screening, and preimplantation genetic testing (PGT). Additionally, it is instrumental in rare disease diagnosis, pharmacogenomics, transplant medicine, and neurological disorder screening, offering personalized insights for treatment decisions. As NGS technology advances, its role in cardiovascular risk assessment, immunogenetics, and population genomics continues to expand, further integrating precision medicine into routine clinical practice.

不含甘油的定制配方服务
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阅读NGS相关博客文章

以下是关于下一代测序(NGS)的最新博客文章:

NGS
试想一下,诊断化验可在室温下运输和储存,无需冷冻或冷藏,同时还能在...
诊断领域正迅速演进,个性化医疗需求的增长成为这一变革的重要驱动力。数十年来,qPCR一直是诊断测试的中流砥柱,因其快速、灵敏和高效的优势广泛应用于常规检测。然而,随着癌症、遗传疾病及新兴传染病等复杂疾病的增多,对能够提供更深层次和更广泛洞察的技术需求也随之增加。
NGS检测和POC检测这些年一直在不断发展,成为了现代医疗保健的基石。传统上,这两者其实在很大程度上是相互对立的
本文讨论了NGS普及化的主要障碍及迈迪安提供的NGS建库解决方案。

参考文献:
Zalis, M., Viana Veloso, G. G., Aguiar Jr., P. N., Gimenes, N., Reis, M. X., Matsas, S., & Ferreira, C. G. (2024).下一代测序对癌症治疗的影响:应用、挑战和未来方向。遗传学前沿》,15,1420190。https://doi.org/10.3389/fgene.2024.1420190

Meric-Bernstam, F., Brusco, L., Daniels, M., Wathoo, C., Bailey, A. M., Strong, L., & Shaw, K. (2019).下一代测序在精准肿瘤学中的临床应用。NPJ Precision Oncology, 3, 4.https://doi.org/10.1038/s41698-019-0073-4

要检测更多改变,可考虑在NGS中添加辅助检测。(n.d.).Targeted Oncology.从 https://www.targetedonc.com/view/to-detect-more-alterations-consider-adding-companion-testing-to-ngs

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