Stool Master Mixes

Stool tests can allow identification of enteric pathogens such as harmful bacteria, fungi, viruses, or parasites down to the genus or species level, fast and accurately in a few hours, rather than the culture-based stool testing that can take several days. They can also be used as a non-invasive method for screening autoimmune conditions and cancer.

The fecal occult blood test (FOBT) has been the most widely used gastrointestinal immunoassay to screen colorectal cancer (CRC) for over three decades, however, recent large-scale studies have shown that the FOBT lacks the sensitivity to detect early stages of CRC. A molecular cancer stool test is a better diagnostic tool as it offers significant improvement in sensitivity and accuracy and can be used to screen for multiple targets and so speed up diagnosis, therefore improving prognosis.

With Meridian’s direct stool master mixes, you can see down to less than 10 copies.

市场上有几种可用于粪便检测的缓冲液，包括磷酸盐缓冲盐水(PBS)、Cary-Blair储存液、粪便运输和复原(STAR)缓冲液和DNA/RNA Shield。

Pre-treatment will depend on what you are looking at, cfDNA may not need pre-treatment, but you may need to heat to 95°C for 5 min and for some bacteria, a Proteinase K step (Proteinase K 55°C for 15 min and then at 98°C for 5 min) or using a non-ionic detergent (such as Tween-20 to final concentration of 0.2% followed by incubation at room temperature for 10 min) may be necessary.

We do not see a performance difference between the two master mixes.

They are compatible with standard cycling conditions and can also be used with very fast cycling conditions, however some assay development may be required for the volume of urine that is needed for a molecular assay.

冻干后的预混液在正确封存的情况下可在常温下稳定保持2年。