Animal-Free, Recombinant Immunoassay Blockers
Immunoassay blockers are used in diagnostic assays to reduce non-specific binding and other interference that can lead to false-positive results. Specifically, interference from human anti-mouse antibodies (HAMA), rheumatoid factor (RH), and heterophilic antibodies (HA) are a major concern in both paired monoclonal sandwich assays and competitive assays. Specialized blockers are required in these assays to reduce any interference and to ensure the assay's accuracy.
Immunoassay blockers are usually produced using animal sources however this presents risks like contamination, batch variability and poor scalability. Recombinant blockers in contrast are 100% animal-free and manufactured under strict controls, enabling precise reproducibility across experiments, and scalability, making it ideal for regulated diagnostic immunoassays.
Meridian offers new recombinant blocker options: Recombinant Mouse-Free IgG and K-Block™.
产品过滤器
Animal-Free, Recombinant Immunoassay Blockers Products (2)
| 姓名 | 种类 | 形式 | 宿主/来源 | 亚型 | 主要应用 | 单位 | Buffer | Immunogen | Recombinant | 说明 | Notes | 安全信息表 | COA | PIS | New Product | Recommended Product | 订购样品 | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| K-BLOCK (TM) | Blocker | Purified | Cell Culture | N/A | Blo | MG | BN1200 | 是 | 安全数据表 | COA/Test Release | 0 | 0 | 订购样品 | |||||||
| Mouse-FREE IgG | Blocker | Purified, Liquid | Cell Culture | N/A | Blo | MG | BN1300 | 是 | 安全数据表 | COA/Test Release | 0 | 0 | 订购样品 |
Top 5 Advantages of Using an Animal-Free Blocker
Enhanced specificity:
Animal-free blockers offer improved specificity over traditional blockers, as they will not cross-react with animal-sourced antigens and antibodies in the assay. The potential for non-specific binding is reduced and the signal-to-background is improved.
Consistent performance:
Animal-derived reagents can have batch-to-batch variability, impacting assay consistency. In contrast, animal-free reagents are more chemically well-defined and contain a finite number of traceable ingredients, resulting in greater reproducibility, regulatory advantages and increased speed-to-market.
Reduced risk of contamination:
Animal-derived components used in traditional blockers can carry contaminants, such as viruses, prions, and endotoxins, that can affect the accuracy of an assay. Animal-free blockers strongly reduce this risk.
Stable and reliable supply chain:
Conventional mouse serum and mouse IgG are typically derived from animal sources, often sourced internationally. Consequently, their availability is susceptible to supply chain disruptions caused by geopolitical events or biological threats. In contrast, recombinant blockers are produced through fully scalable and tightly controlled manufacturing processes, enabling flexible production volumes and ensuring resilience against disruptions affecting animal populations.
Regulatory compliance:
Regulations such as the European Union (EU) Directive2010/63/EU, are encouraging the use of animal-free reagents. Using an animal-free blocker aligns with the ethical concerns related to using animals in science.
Proven Results for IVD
Immunoassay blockers are used in diagnostic assays to reduce non-specific binding and other interference that can lead to false-positive results. Meridian’s newest animal-free solutions, Recombinant Mouse IgG and K-Block™, have proven high performance against similar animal-based commercial blockers from top competitors:
Figure 3. A double mouse monoclonal sandwich assay was used to compare the performance of Meridian’s Recombinant Mouse-Free IgG against serum-derived purified native mouse IgG and two competitor HAMA blockers. (A) Human HAMA-positive or (B) RF-positive serum was preincubated with the individual blockers for 20 minutes at room temperature. Blocker concentrations ranged from 0.0003–1.25 μg/mL for the HAMA assay and 0.0195–80 μg/mL for the RF assay. The effectiveness of each blocker was determined by comparing the relative suppression of the HAMA or RF signal – lower EC50 values indicate greater interference suppression. The results demonstrate that Mouse-Free IgG is significantly more effective than native mouse IgG and the two competitor blockers at blocking HAMA and RF interference.
K-Block Performance:
A double mouse monoclonal sandwich assay was used to compare the performance of Meridian Bioscience’s K-Block™ (100% animal-free, recombinant blocker, MLS Cat. No. BN1200) against serum-derived purified native mouse IgG and two competitor recombinant mouse IgG HAMA blockers. (A) Human HAMA-positive or (B) RF-positive serum was preincubated with the individual blockers for 20 minutes at room temperature. Blocker concentrations ranged from 0.0001-1.25 µg/ml for the HAMA assay and 0.01-80 µg/ml for the RF assay. The effectiveness of each blocker was determined by comparing the relative suppression of the HAMA signal to the signal of samples with no blocker. The results demonstrate that at lower concentrations, K-Block™ was more effective than native mouse IgG and the two competitors’ recombinant HAMA blockers. At higher concentrations, K-Block™ is as effective as the other blockers.
产品资料
Meridian K-Block CatalogMeridian K-Block Catalog
Mouse-FREE IgGMouse-FREE IgG
Frequently Asked Questions
Immunoassay Interference Blockers
Mouse IgG can be added up to 10X the concentration of the monoclonal antibody being used in the assay to increase blocking effectiveness.
Recombinant Mouse-Free IgG is specifically engineered to represent all the major isotypes in native mouse IgG, and has optimized ratios to ensure peak performance. As a result, it is 20% more effective than native mouse IgG at blocking HAMA and RF.
For diluted samples, K-Block should be used between 0.5 µg/ml and 20 µg/ml. For non-diluted samples, K-Block should be used between 5 µg/ml and 200 µg/ml final concentration.
For best performance, the blocker should be included as part of the sample or conjugate diluent. In lateral flow assays, the HAMA blocker may be added to the conjugate pad, to a sample diluent or pretreatment buffer, and/or applied to the membrane as a blocking stripe located before the test stripe.
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Animal-Free Immunoassay Blockers
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