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Glycerol-Free DNA Helicase (HC) Kit

Glycerol-Free DNA Helicase (HC) is a high-concentration, glycerol-free mix multi-domain DNA Helicase enzyme that uses ATP hydrolysis to unwind DNA duplexes with 3’ to 5’ polarity. It functions with the addition of the accessory protein, MutL, supplied with the kit. It is active on a wide range of DNA substrates and is compatible with lyophilization, making it ideal for use in point-of-care applications.

Key Product Features

  • Unwinds double-stranded DNA

  • Operates at 37°C, ideal for mesophilic diagnostic workflows

  • Glycerol-free, for developing lyophilized assays

Description

Glycerol-Free DNA Helicase (HC) Kit (MDX241) is a multi-domain enzyme with both ATPase and helicase activities. It unwinds double-stranded DNA with 3’ to 5’ with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Helicase works in coordination with an accessory protein, methyl-directed mismatch repair (MutL) protein that stimulates the helicase activity.

Glycerol-Free DNA Helicase (HC) has been optimized to deliver excellent, stable performance without glycerol, allowing you to deliver reliable results with the added capability for lyophilization. This gives you the flexibility to confidently store mixtures at ambient temperature or produce diagnostic assays that rely on miniaturized reaction components, such as point-of-care assays.

Schematic of helicase-dependent amplification using Helicase, Mutl, DNA polymerase, primers and dNTPs with temperatures up to 37°C.

Schematic of helicase-dependent amplification using Helicase, Mutl, DNA polymerase, primers and dNTPs with temperatures up to 37°C.

Glycerol-Free DNA Helicase (HC), MDX241

Application: Specimen Type: 脱氧核糖核酸 Concentration: 50 微克/毫升 High Concentration: 是 Wet:
Glycerol-Free: Lyo-Ready: Air-Dryable: Dryable: 需要辅料 Sustainability: 不含甘油

Glycerol-free, high concentration enzyme used to unwind double-stranded DNA with the help of an accessory protein, allowing you to deliver reliable results with the added capability for lyophilization. MutL

Available in 20 μL (1 µg) or 200 μL (10 µg) aliquots

FAQs: Glycerol-Free DNA Helicase (HC) Kit

The Lyo-Ready Thermostable DNA Helicase Reaction Buffer, 5x contains ATP.

No, Glycerol-Free DNA Helicase (HC) can unwind any dsDNA structure.

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