Description
Glycerol-Free DNA Helicase (HC) Kit (MDX241) is a multi-domain enzyme with both ATPase and helicase activities. It unwinds double-stranded DNA with 3’ to 5’ with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Helicase works in coordination with an accessory protein, methyl-directed mismatch repair (MutL) protein that stimulates the helicase activity.
Glycerol-Free DNA Helicase (HC) has been optimized to deliver excellent, stable performance without glycerol, allowing you to deliver reliable results with the added capability for lyophilization. This gives you the flexibility to confidently store mixtures at ambient temperature or produce diagnostic assays that rely on miniaturized reaction components, such as point-of-care assays.
Schematic of helicase-dependent amplification using Helicase, Mutl, DNA polymerase, primers and dNTPs with temperatures up to 37°C.
Glycerol-Free DNA Helicase (HC), MDX241
Glycerol-free, high concentration enzyme used to unwind double-stranded DNA with the help of an accessory protein, allowing you to deliver reliable results with the added capability for lyophilization. MutL
Documents & Resources
Catalogs & Brochures
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Lyo-Ready & Air-Dryable LAMP & RT-LAMP Master MixesLyo-Ready & Air-Dryable LAMP & RT-LAMP Master Mixes
FAQs: Glycerol-Free DNA Helicase (HC) Kit
The Lyo-Ready Thermostable DNA Helicase Reaction Buffer, 5x contains ATP.
No, Glycerol-Free DNA Helicase (HC) can unwind any dsDNA structure.
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