Isothermal Amplification
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Products (23)
Name | Details | Application | Catalog | Order a Sample | Sizes | Description | Wet(Liquid) | Air-Dryable | Name | Glycerol-Free | Lyo-Ready | Concentration | High Concentration | Detail | Safety Data Sheet | COA/Test Release | User Guide | Product Handling Guide | Product Manual | ||
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Lyo-Ready Direct RNA/DNA LAMP Blood, 4x | Blood, Plasma, Serum, LAMP Mixes | RT-LAMP | MDX125 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 10% whole blood, 10% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications. |
Yes | No | Lyo-Ready | Yes | Yes | 4x | Yes | View Details | COA/Test Release | User Guide | Product Handling Guide | ||||
Lyo-Ready Direct DNA LAMP Saliva, 4x | Saliva, Sputum, LAMP Mixes | LAMP | MDX134 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections. |
Yes | No | Lyo-Ready | Yes | Yes | 4x | Yes | View Details | Safety Data Sheet | COA/Test Release | User Guide | Product Handling Guide | |||
Lyo-Ready Direct RNA/DNA LAMP Saliva, 4x | Saliva, Sputum, LAMP Mixes | RT-LAMP | MDX135 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections. |
Yes | No | Lyo-Ready | Yes | Yes | 4x | Yes | View Details | Safety Data Sheet | COA/Test Release | User Guide | Product Handling Guide | |||
Lyo-Ready Direct DNA LAMP Stool, 4x | Stool, LAMP Mixes | LAMP | MDX144 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 20% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses. |
Yes | No | Lyo-Ready | Yes | Yes | 4x | Yes | View Details | Safety Data Sheet | COA/Test Release | User Guide | Product Handling Guide | |||
Lyo-Ready Direct RNA/DNA LAMP Stool, 4x | Stool, LAMP Mixes | RT-LAMP | MDX145 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 5% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses. |
Yes | No | Lyo-Ready | Yes | Yes | 4x | Yes | View Details | Safety Data Sheet | COA/Test Release | User Guide | Product Handling Guide | |||
Air-Dryable Direct DNA LAMP Blood, 4x | Blood, Plasma, Serum, LAMP Mixes | LAMP | MDX126 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 10% whole blood, 10% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications. |
Yes | Yes | Air-Dryable | Yes | No | 4x | Yes | View Details | Safety Data Sheet | COA/Test Release | User Guide | Product Handling Guide | |||
Lyo-Ready Direct DNA LAMP Urine, 4x | Urine, LAMP Mixes | LAMP | MDX154 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer. |
Yes | No | Lyo-Ready | Yes | Yes | 4x | Yes | View Details | COA/Test Release | User Guide | Product Handling Guide | ||||
Lyo-Ready Direct RNA/DNA LAMP Urine, 4x | Urine, LAMP Mixes | RT-LAMP | MDX155 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer. |
Yes | No | Lyo-Ready | Yes | Yes | 4x | Yes | View Details | COA/Test Release | User Guide | Product Handling Guide | ||||
Glycerol-Free T7 RNA Polymerase (HC) | Isothermal Enzymes, Air-Dryable & Lyo-Ready Enzymes | NASBA | MDX201 | Order a Sample | Available in 50,000 Unit or 500,000 Unit aliquots | Glycerol-free, high concentration DNA-dependant RNA Polymerase that requires a T7 promoter sequence, it an ideal enzyme for in-vitro synthesis of labeled or unlabeled RNA for research, diagnostic or clinical applications. Ideal for lyophilization. |
Yes | Requires excipients | Requires excipients | Yes | Requires excipients | 1,000 U/µL | Yes | View Details | COA/Test Release | Product Handling Guide | |||||
Glycerol-Free RNase H (HC) | Reverse Transcriptase, NGS Enzymes, NASBA, Air-Dryable & Lyo-Ready Enzymes | RT-qPCR | MDX202 | Order a Sample | Available in 100 µL (2,500 Unit) or 250 µL (12,500 Unit) aliquots | Glycerol-free, high concentration RNA-specific endonuclease that hydrolyzes the phosphodiester bonds of RNA, when hybridized to DNA. Ideal for lyophilization. |
Yes | Requires excipients | Requires excipients | Yes | Requires excipients | 50 U/µL | Yes | View Details | COA/Test Release | Product Handling Guide | |||||
Glycerol-Free Phi29 DNA Polymerase (HC) | Isothermal Enzymes, Air-Dryable & Lyo-Ready Enzymes | WGA | MDX240 | Order a Sample | Available in 20 µL (2,000 Unit) or 100 µL (10,000 Unit) aliquots | Glycerol-free, high concentration enzyme with exceptional strand displacement and processive synthesis properties, ideal for rolling-circle amplification (RCA). |
Yes | Requires excipients | Requires excipients | Yes | Requires excipients | 100 U/?L | Yes | View Details | |||||||
Glycerol-Free Thermostable DNA Helicase (HC) | Isothermal Enzymes, Air-Dryable & Lyo-Ready Enzymes | tHAD | MDX242 | Order a Sample | Available in 20 ?L (1 µg) or 200 ?L (10 µg) aliquots | Glycerol-free, high concentration enzyme used with in isothermal amplification method such as LAMP, RPA, RCA, SDA and tHDA) |
Yes | Requires excipients | Requires excipients | Yes | Requires excipients | 50 µg/mL | Yes | View Details | Product Handling Guide | ||||||
Lyo-Ready LAMP Mix, 4x | LAMP Mixes | LAMP | MDX097 | Order a Sample | Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots | Glycerol-free mix for isothermal applications such as LAMP, containing Bst DNA polymerase, reaction buffer, dNTPs and lyo-compatible excipients, ideal for developing ambient temperature, LAMP assays. |
Yes | No | Lyo-Ready | Yes | Yes | 4x | Yes | View Details | COA/Test Release | User Guide | Product Handling Guide | ||||
Lyo-Ready RT-LAMP 1-Step Mix, 4x | LAMP Mixes | RT-LAMP | MDX108 | Order a Sample | Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots | Glycerol-free mix for isothermal applications such as RT-LAMP, containing Bst DNA polymerase, reverse transcriptase, reaction buffer, dNTPs and lyo-compatible excipients, ideal for developing ambient temperature, LAMP assays. |
Yes | No | Lyo-Ready | Yes | Yes | 4x | Yes | View Details | COA/Test Release | User Guide | Product Handling Guide | ||||
Air-Dryable RT-LAMP 1-Step Mix, 4x | LAMP Mixes | RT-LAMP | MDX118 | Order a Sample | Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots | Glycerol-free mix for isothermal applications such as RT-LAMP, containing Bst DNA polymerase, reverse transcriptase, reaction buffer, dNTPs and air-dryable excipients, ideal for developing ambient temperature, LAMP assays. |
Yes | Yes | Air-Dryable | Yes | No | 4x | Yes | View Details | Safety Data Sheet | COA/Test Release | User Guide | Product Handling Guide | |||
Air-Dryable DNA LAMP | LAMP Mixes | LAMP | MDX119 | Order a Sample | Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots | Glycerol-free mix for isothermal applications such as LAMP, containing Bst DNA polymerase, reaction buffer, dNTPs and air-dryable excipients, ideal for developing ambient temperature, LAMP assays. |
Yes | Yes | Air-Dryable | Yes | No | 4x | Yes | View Details | COA/Test Release | User Guide | Product Handling Guide | ||||
Lyo-Ready Direct DNA LAMP Blood, 4x | Blood, Plasma, Serum, LAMP Mixes | LAMP | MDX124 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 20% whole blood, 20% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications. |
Yes | No | Lyo-Ready | Yes | Yes | 4x | Yes | View Details | Safety Data Sheet | COA/Test Release | User Guide | Product Handling Guide | |||
Bst Reaction Buffer, 10x | Buffer, Bst Enzymes & Buffers | LAMP | MDX076 | Order a Sample | Available in 5 mL or 100 mL aliquots | Reaction Buffer optimized for use with Bst DNA Polymerase. |
Yes | Requires excipients | Requires excipients | Yes | Requires excipients | 10x | No | View Details | Safety Data Sheet | COA/Test Release | Product Handling Guide | ||||
Enzyme Dilution Buffer, 1x | Buffer, Bst Enzymes & Buffers | PCR | MDX078 | Order a Sample | Available in 5 mL or 100 mL aliquots | Glycerol containing1x dilution buffer, for the dilution of enzymes to reaction concentration. |
Yes | No | No | No | No | 1x | No | View Details | Safety Data Sheet | COA/Test Release | Product Handling Guide | ||||
Enzyme Dilution Buffer, 10x (Glycerol free) | Buffer, Bst Enzymes & Buffers, Air-Dryable & Lyo-Ready Enzymes | PCR | MDX080 | Order a Sample | Available in 5 mL or 100 mL aliquots | Glycerol-free,10x dilution buffer, for the dilution of enzymes to reaction concentration. |
Yes | Yes | Air-Dryable,Lyo-Ready | Yes | Yes | 10x | No | View Details | Safety Data Sheet | COA/Test Release | Product Handling Guide | ||||
Bst DNA Polymerase | Isothermal DNA Polymerase, Bst Enzymes & Buffers | LAMP | MDX012 | Order a Sample | Available in 1 mL or 10 mL aliquots | DNA polymerase (exo-), with strand-displacement properties. used for Isothermal DNA amplification such as LAMP (Loop-mediated Isothermal Amplification). |
Yes | No | No | No | No | 8 units/mL | No | View Details | Safety Data Sheet | COA/Test Release | Product Handling Guide | ||||
High Conc. Glycerol-Free Bst | Isothermal DNA Polymerase, Bst Enzymes & Buffers, Air-Dryable & Lyo-Ready Enzymes | LAMP | MDX018 | Order a Sample | Available in 80 µL or 800 µL aliquots | High concentration glycerol-free DNA polymerase (exo-), with strand-displacement properties. used for Isothermal DNA amplification such as LAMP (Loop-mediated Isothermal Amplification). |
Yes | Yes | Requires excipients | Yes | Yes | 100 units/mL | Yes | View Details | Safety Data Sheet | COA/Test Release | Product Handling Guide | ||||
Inhibitor-Tolerant Bst Buffer | Buffer, Bst Enzymes & Buffers | LAMP | MDX019 | Order a Sample | Available in 2 mL or 100 mL aliquots | Inhibitor-Tolerant Bst Buffer is specially designed to overcome the inhibition present in samples in direct LAMP reactions. |
Yes | Yes | Requires excipients | Yes | Yes | 10x | No | View Details | Safety Data Sheet | COA/Test Release | Product Handling Guide |
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Sample Specific Inhibitor Tolerant LAMP Mixes
Loop mediated isothermal amplification LAMP, for example, uses four to six primers that recognize several specific regions in the target DNA. The reaction is initiated by a strand-displacement polymerase such as Bst DNA polymerase and two specially designed primers which form loop structures to facilitate subsequent rounds of amplification producing high levels of DNA. LAMP reactions are very robust and inhibitor-tolerant, producing extremely large amounts of DNA that can be visualized by the eye (e.g. as a precipitate or color change). Specifically, 1 to 10 copies of DNA can be amplified to 109 to 1010 copies within 30 minutes, producing assays with excellent sensitivity and specificity.
For even greater tolerance, Meridian has also developed sample specific inhibitor tolerant LAMP mixes, designed to be used for direct amplification from crude lysates or inhibitor-rich samples, with the ability to tolerate even higher levels of inhibitors present in samples. In addition these mixes can be used as wet mixes or dried by lyophilization or air-drying for room temperature shipping and storage, extended shelf-life, and increased flexibility in sample volume, making them ideal for POCT development where minimal sample processing and fast turn-around times are essential.
Advanced Isothermal Amplification Solutions
Catalogs & Brochures
Lyo-Ready LAMP Master Mix Lyo-Ready LAMP Master Mix
Lyo-Ready Direct DNA LAMP Blood Master Mixes Lyo-Ready Direct DNA LAMP Blood Master Mixes
Lyo-Ready Direct Stool Urine LAMP Master Mixes Lyo-Ready Direct Stool Urine LAMP Master Mixes
Specimen-specific and Universal qPCR & LAMP Master Mixes Specimen-specific and Universal qPCR & LAMP Master Mixes
Lyo-Ready RT-LAMP 1-Step Master Mix Lyo-Ready RT-LAMP 1-Step Master Mix
Lyo-Ready Direct LAMP Saliva Master Mixes Lyo-Ready Direct LAMP Saliva Master Mixes
Lyo-Ready Direct LAMP Urine Lyo-Ready Direct LAMP Urine
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