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Isothermal amplification (including HAD, MCA, and LAMP) is a DNA amplification technique that has played an increasingly important role in point-of-care (POC) diagnostics. It offers several advantages over qPCR and other amplification techniques in terms of sample-to-answer time, sensitivity, specificity, cost, robustness, and accessibility, making it ideal for field-deployable diagnostics in resource-limited regions. Isothermal amplification assays have been used for numerous applications including the detection of pathogens such as salmonella and malaria, GM crop contamination, and forensics to specifically detect human DNA.
| Name | Details | Application | Catalog | Order a Sample | Sizes | Description | Wet(Liquid) | Air-Dryable | Name | Glycerol-Free | Lyo-Ready | Concentration | High Concentration | Detail | Safety Data Sheet | COA/Test Release | User Guide | Product Handling Guide | Product Manual | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Glycerol-Free Thermostable DNA Helicase (HC) | DNA | LAMP, RPA, RCA, SDA | MDX242 | Order a Sample | Available in 20 µL (1 µg) or 200 µL (10 µg) aliquots | Glycerol-free, high concentration enzyme used to unwind double-stranded DNA. It operates at up to 65°C, ideal for demanding diagnostic workflows such as NGS library preparation, SNP detection and mutation analysis | Yes | Yes | Requires excipients | Yes | Yes | 50 µg/mL | Yes | View Details | Safety Data Sheet | Certifate of Analysis | Product Handling Guide | ||||
| Glycerol-Free Phi29 DNA Polymerase (HC) | DNA | RCA, WGA | MDX240 | Order a Sample | Available in 20 µL (2,000 Unit) or 100 µL (10,000 Unit) aliquots | Glycerol-free, high concentration enzyme with exceptional strand displacement and processive synthesis properties, ideal for rolling-circle amplification (RCA). | Yes | Yes | Requires excipients | Yes | Yes | 100 U/?L | Yes | View Details | Safety Data Sheet | Certifate of Analysis | Product Handling Guide | ||||
| Glycerol-Free RNase H (HC) | RNA | RT-qPCR | MDX202 | Order a Sample | Available in 100 µL (2,500 Unit) or 250 µL (12,500 Unit) aliquots | Glycerol-free, high concentration RNA-specific endonuclease that hydrolyzes the phosphodiester bonds of RNA, when hybridized to DNA. Ideal for lyophilization. | Yes | Yes | Requires excipients | Yes | Yes | 50 U/µL | Yes | View Details | Certifate of Analysis | Product Handling Guide | |||||
| Glycerol-Free T7 RNA Polymerase (HC) | RNA | NASBA | MDX201 | Order a Sample | Available in 50,000 Unit or 500,000 Unit aliquots | Glycerol-free, high concentration DNA-dependant RNA Polymerase that requires a T7 promoter sequence, it an ideal enzyme for in-vitro synthesis of labeled or unlabeled RNA for research, diagnostic or clinical applications. Ideal for lyophilization. | Yes | Yes | Requires excipients | Yes | Yes | 1,000 U/µL | Yes | View Details | Safety Data Sheet | Certifate of Analysis | Product Handling Guide | ||||
| Lyo-Ready Direct RNA/DNA LAMP Urine, 4x | Urine, RNA | RT-LAMP | MDX155 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer. | Yes | Yes | Lyo-Ready | Yes | Yes | 4x | Yes | View Details | Safety Data Sheet | Certifate of Analysis | User Guide | Product Handling Guide | |||
| Lyo-Ready Direct DNA LAMP Urine, 4x | Urine, DNA | LAMP | MDX154 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer. | Yes | Yes | Lyo-Ready | Yes | Yes | 4x | Yes | View Details | Safety Data Sheet | Certifate of Analysis | User Guide | Product Handling Guide | |||
| Lyo-Ready Direct RNA/DNA LAMP Saliva, 4x | Saliva, Sputum, RNA | RT-LAMP | MDX135 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections. | Yes | Yes | Lyo-Ready | Yes | Yes | 4x | Yes | View Details | Safety Data Sheet | Certifate of Analysis | User Guide | Product Handling Guide | |||
| Lyo-Ready Direct RNA/DNA LAMP Stool, 4x | Stool, RNA | RT-LAMP | MDX145 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 5% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses. | Yes | Yes | Lyo-Ready | Yes | Yes | 4x | Yes | View Details | Safety Data Sheet | Certifate of Analysis | User Guide | Product Handling Guide | |||
| Lyo-Ready Direct DNA LAMP Stool, 4x | Stool, DNA | LAMP | MDX144 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 20% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses. | Yes | Yes | Lyo-Ready | Yes | Yes | 4x | Yes | View Details | Safety Data Sheet | Certifate of Analysis | User Guide | Product Handling Guide | |||
| Lyo-Ready Direct DNA LAMP Saliva, 4x | Saliva, Sputum, DNA | LAMP | MDX134 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections. | Yes | Yes | Lyo-Ready | Yes | Yes | 4x | Yes | View Details | Safety Data Sheet | Certifate of Analysis | User Guide | Product Handling Guide | |||
| Air-Dryable Direct DNA LAMP Blood, 4x | Blood, Plasma, Serum, DNA | LAMP | MDX126 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 10% whole blood, 10% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications. | Yes | Yes | Air-Dryable | Yes | No | 4x | Yes | View Details | Safety Data Sheet | Certifate of Analysis | User Guide | Product Handling Guide | |||
| Lyo-Ready Direct RNA/DNA LAMP Blood, 4x | Blood, Plasma, Serum, RNA | RT-LAMP | MDX125 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 10% whole blood, 10% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications. | Yes | Yes | Lyo-Ready | Yes | Yes | 4x | Yes | View Details | Safety Data Sheet | Certifate of Analysis | User Guide | Product Handling Guide | |||
| Lyo-Ready Direct DNA LAMP Blood, 4x | Blood, Plasma, Serum, DNA | LAMP | MDX124 | Order a Sample | Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots | Inhibitor tolerance to 20% whole blood, 20% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications. | Yes | Yes | Lyo-Ready | Yes | Yes | 4x | Yes | View Details | Safety Data Sheet | Certifate of Analysis | User Guide | Product Handling Guide | |||
| Air-Dryable DNA LAMP | DNA | LAMP | MDX119 | Order a Sample | Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots | Glycerol-free mix for isothermal applications such as LAMP, containing Bst DNA polymerase, reaction buffer, dNTPs and air-dryable excipients, ideal for developing ambient temperature, LAMP assays. | Yes | Yes | Air-Dryable | Yes | No | 4x | Yes | View Details | Certifate of Analysis | User Guide | Product Handling Guide | ||||
| Air-Dryable RT-LAMP 1-Step Mix, 4x | RNA | RT-LAMP | MDX118 | Order a Sample | Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots | Glycerol-free mix for isothermal applications such as RT-LAMP, containing Bst DNA polymerase, reverse transcriptase, reaction buffer, dNTPs and air-dryable excipients, ideal for developing ambient temperature, LAMP assays. | Yes | Yes | Air-Dryable | Yes | No | 4x | Yes | View Details | Safety Data Sheet | Certifate of Analysis | User Guide | Product Handling Guide | |||
| Lyo-Ready RT-LAMP 1-Step Mix, 4x | RNA | RT-LAMP | MDX108 | Order a Sample | Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots | Glycerol-free mix for isothermal applications such as RT-LAMP, containing Bst DNA polymerase, reverse transcriptase, reaction buffer, dNTPs and lyo-compatible excipients, ideal for developing ambient temperature, LAMP assays. | Yes | Yes | Lyo-Ready | Yes | Yes | 4x | Yes | View Details | Safety Data Sheet | Certifate of Analysis | User Guide | Product Handling Guide | |||
| Lyo-Ready LAMP Mix, 4x | DNA | LAMP | MDX097 | Order a Sample | Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots | Glycerol-free mix for isothermal applications such as LAMP, containing Bst DNA polymerase, reaction buffer, dNTPs and lyo-compatible excipients, ideal for developing ambient temperature, LAMP assays. | Yes | Yes | Lyo-Ready | Yes | Yes | 4x | Yes | View Details | Certifate of Analysis | User Guide | Product Handling Guide | ||||
| Enzyme Dilution Buffer, 1x | DNA | PCR | MDX078 | Order a Sample | Available in 5 mL or 100 mL aliquots | Glycerol containing1x dilution buffer, for the dilution of enzymes to reaction concentration. | Yes | No | No | Yes | No | 1x | Yes | View Details | Safety Data Sheet | Certifate of Analysis | Product Handling Guide | ||||
| Bst Reaction Buffer, 10x | DNA | LAMP | MDX076 | Order a Sample | Available in 5 mL or 100 mL aliquots | Reaction Buffer optimized for use with Bst DNA Polymerase. | Yes | Yes | Requires excipients | Yes | Yes | 10x | Yes | View Details | Safety Data Sheet | Certifate of Analysis | Product Handling Guide | ||||
| Enzyme Dilution Buffer, 10x (Glycerol free) | DNA | PCR | MDX080 | Order a Sample | Available in 5 mL or 100 mL aliquots | Glycerol-free,10x dilution buffer, for the dilution of enzymes to reaction concentration. | Yes | Yes | Air-Dryable,Lyo-Ready | Yes | Yes | 10x | Yes | View Details | Safety Data Sheet | Certifate of Analysis | Product Handling Guide | ||||
| Bst DNA Polymerase | DNA | LAMP | MDX012 | Order a Sample | Available in 1 mL or 10 mL aliquots | DNA polymerase (exo-), with strand-displacement properties. used for Isothermal DNA amplification such as LAMP (Loop-mediated Isothermal Amplification). | Yes | No | No | Yes | No | 8 units/mL | Yes | View Details | Safety Data Sheet | Certifate of Analysis | Product Handling Guide | ||||
| Inhibitor-Tolerant Bst Buffer | DNA | LAMP | MDX019 | Order a Sample | Available in 2 mL or 100 mL aliquots | Inhibitor-Tolerant Bst Buffer is specially designed to overcome the inhibition present in samples in direct LAMP reactions. | Yes | Yes | Requires excipients | Yes | Yes | 10x | Yes | View Details | Safety Data Sheet | Certifate of Analysis | Product Handling Guide | ||||
| High Conc. Glycerol-Free Bst | DNA | LAMP | MDX018 | Order a Sample | Available in 80 µL or 800 µL aliquots | High concentration glycerol-free DNA polymerase (exo-), with strand-displacement properties. used for Isothermal DNA amplification such as LAMP (Loop-mediated Isothermal Amplification). | Yes | Yes | Requires excipients | Yes | Yes | 100 units/mL | Yes | View Details | Safety Data Sheet | Certifate of Analysis | Product Handling Guide | ||||
| Lyo-Ready Direct RNA NASBA Blood | Blood, Plasma, Serum, RNA | NASBA | MDX220 | Order a Sample | Available in 400 mL (50 Rxns) or 2 mL (250 Rxns) aliquots | Single pot, one-step Nucleic Acid Sequence-Based Amplification (NASBA) for reliable isothermal amplification of RNA transcripts from crude or purified blood samples, ideal for POC testing | Yes | Yes | Lyo-Ready | Yes | Yes | 2.5x | Yes | View Details | Safety Data Sheet | Certifate of Analysis | Product Handling Guide | ||||
| Lyo-Ready Direct RNA NASBA Saliva | Saliva, Sputum, RNA | NASBA | MDX221 | Order a Sample | Available in 400 mL (50 Rxns) or 2 mL (250 Rxns) aliquots | Single pot, one-step Nucleic Acid Sequence-Based Amplification (NASBA) for reliable isothermal amplification of RNA transcripts from crude or purified saliva samples, ideal for POC testing | Yes | Yes | Lyo-Ready | Yes | Yes | 2.5x | Yes | View Details | Safety Data Sheet | Certifate of Analysis | Product Handling Guide | ||||
| Lyo-Ready Direct RNA NASBA Stool | Stool, RNA | NASBA | MDX222 | Order a Sample | Available in 400 mL (50 Rxns) or 2 mL (250 Rxns) aliquots | Single pot, one-step Nucleic Acid Sequence-Based Amplification (NASBA) for reliable isothermal amplification of RNA transcripts from crude or purified stool samples, ideal for POC testing | Yes | Yes | Lyo-Ready | Yes | Yes | 2.5x | Yes | View Details | Safety Data Sheet | Certifate of Analysis | Product Handling Guide | ||||
| Lyo-Ready Direct RNA NASBA Urine | Urine, RNA | NASBA | MDX223 | Order a Sample | Available in 400 mL (50 Rxns) or 2 mL (250 Rxns) aliquots | Single pot, one-step Nucleic Acid Sequence-Based Amplification (NASBA) for reliable isothermal amplification of RNA transcripts from crude or purified urine samples, ideal for POC testing | Yes | Yes | Lyo-Ready | Yes | Yes | 2.5x | Yes | View Details | Safety Data Sheet | Certifate of Analysis | Product Handling Guide | ||||
| Glycerol-Free DNA Helicase (HC) | DNA | LAMP, RPA, RCA, SDA | MDX241 | Order a Sample | Available in 20 ?L (1 µg) or 200 ?L (10 µg) aliquots | Glycerol-free, high concentration enzyme used to unwind double-stranded DNA with the help of an accessory protein, allowing you to deliver reliable results with the added capability for lyophilization. MutL | Yes | Yes | Requires excipients | Yes | Yes | 50 µg/mL | Yes | View Details | Safety Data Sheet | Certifate of Analysis | Product Handling Guide |
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Loop mediated isothermal amplification LAMP, for example, uses four to six primers that recognize several specific regions in the target DNA. The reaction is initiated by a strand-displacement polymerase such as Bst DNA polymerase and two specially designed primers which form loop structures to facilitate subsequent rounds of amplification producing high levels of DNA. LAMP reactions are very robust and inhibitor-tolerant, producing extremely large amounts of DNA that can be visualized by the eye (e.g. as a precipitate or color change). Specifically, 1 to 10 copies of DNA can be amplified to 109 to 1010 copies within 30 minutes, producing assays with excellent sensitivity and specificity.
For even greater tolerance, Meridian has also developed sample specific inhibitor tolerant LAMP mixes, designed to be used for direct amplification from crude lysates or inhibitor-rich samples, with the ability to tolerate even higher levels of inhibitors present in samples. In addition these mixes can be used as wet mixes or dried by lyophilization or air-drying for room temperature shipping and storage, extended shelf-life, and increased flexibility in sample volume, making them ideal for POCT development where minimal sample processing and fast turn-around times are essential.
Meridian offers diagnostic manufacturers the complete solution with over 3,000 antigens and antibodies covering more than 500 diseases and an extensive range of specialty qPCR/RT-qPCR master mixes and molecular enzymes all manufactured under ISO13485 regulations.
Large Sample Size
Lot Reservation
ISO13485
Lyo-Ready Direct DNA LAMP Blood Master Mixes
Lyo-Ready Direct Stool Urine LAMP Master Mixes
Specimen-specific and Universal qPCR & LAMP Master Mixes
Lyo-Ready RT-LAMP 1-Step Master Mix
Lyo-Ready Direct LAMP Saliva Master Mixes
Enhancing Isothermal Amplification with Cutting-Edge Raw Materials
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