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DNA-RNA Extraction Controls

Meridian’s DNA Extraction Controls use E. coli cells and RNA Extraction Controls use artificial micelles, of a known concentration that contain quantitative PCR positive control DNA or RNA sequence (with no known homology to any organism, so that it does not interfere with the detection of the sample target sequence). Their composition closely resembles a test sample and serves as a full-process PCR positive control, monitoring the success of an assay from lysis/extraction to amplification.
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NameDetailsApplicationCatalogOrder a SampleSizesDescriptionWet(Liquid)Air-DryableNameGlycerol-FreeLyo-ReadyConcentrationHigh ConcentrationDetailSafety Data SheetCOA/Test ReleaseUser GuideProduct Handling GuideProduct Manual
VLP-RNA Extraction Control RedRNART-qPCRMDX068Order a SampleAvailable in 1 mL (~1×10^4 copies/µL) and 20 mL (~1×10^4 copies/µL) aliquotsAn Internal Control RNA sequence, with no known homology to any organism, encapsidated in a virus-like particle and a VLP Detection Mix containing primers and probe to the internal control RNA sequence.YesYesAir-Dryable,Lyo-ReadyYesYes10^4 copies/µLYesView DetailsSafety Data SheetCertifate of AnalysisProduct Handling Guide
VLP-RNA Extraction Control OrangeRNART-qPCRMDX069Order a SampleAvailable in 1 mL (~1×10^4 copies/µL) and 20 mL (~1×10^4 copies/µL) aliquotsAn Internal Control RNA sequence, with no known homology to any organism, encapsidated in a virus-like particle and a VLP Detection Mix containing primers and probe to the internal control RNA sequence.YesYesAir-Dryable,Lyo-ReadyYesYes10^4 copies/µLYesView DetailsSafety Data SheetCertifate of AnalysisProduct Handling Guide
qPCR Extraction Control RedDNAqPCRMDX026Order a SampleAvailable in 10 mL (2,000 Rxns) aliquotsControl used to monitor the success of a qPCR assay from lysis/extraction to amplification and reduces the chance of obtaining false negative results from the sample DNA.YesNoNoYesNo25xYesView DetailsSafety Data SheetCertifate of AnalysisProduct Handling Guide
qPCR Extraction Control OrangeDNAqPCRMDX027Order a SampleAvailable in 10 mL (2,000 Rxns) aliquotsControl used to monitor the success of a qPCR assay from lysis/extraction to amplification and reduces the chance of obtaining false negative results from the sample DNA.YesNoNoYesNo25xYesView DetailsSafety Data SheetCertifate of AnalysisProduct Handling Guide
RT-qPCR Extraction Control RedRNART-qPCRMDX028Order a SampleAvailable in 1 mL (500 Rxns) aliquotsControl used to monitor the success of an RT-qPCR assay from lysis/extraction to amplification and reduces the chance of obtaining false negative results from the sample RNA.YesNoNoYesNo25xYesView DetailsSafety Data SheetCertifate of AnalysisProduct Handling Guide
RT-qPCR Extraction Control OrangeRNART-qPCRMDX029Order a SampleAvailable in 1 mL (500 Rxns) aliquotsControl used to monitor the success of an RT-qPCR assay from lysis/extraction to amplification and reduces the chance of obtaining false negative results from the sample RNA.YesNoNoYesNo25xYesView DetailsSafety Data SheetCertifate of AnalysisProduct Handling Guide

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  • Extraction control undergoes the same processing as test sample.
  • Confirms successes of extraction step and monitors co-purification of PCR inhibitors.
  • Probe based designed specifically for multiplex qPCR/RT-qPCR assays.

The extraction controls comprise an internal control sequence in use E. coli cells or artificial micelles and a control mix (containing specific primer and probe (emission wavelength = 560nm or 670nm)) for qPCR detection. The cells are added to the sample or lysis buffer and lysed and extracted with the sample for downstream amplification. The detection of extraction control acts as a PCR positive control and confirms the success of the extraction and amplification steps, avoiding the misinterpretation of a PCR false negative result.

Catalogs & Brochures

Reagent Solutions for Molecular Diagnostics</a >

Reagent Solutions for Molecular Diagnostics

qPCR Extraction Controls</a >

qPCR Extraction Controls

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FAQs

What are the differences / advantages in comparison with other methods like measurement of housekeeping gene, internal control / spike in controls?
The benefit of the artificial Extraction Control in evaluation of the extraction process is the possibility to validate sample extraction process. Signal derived from the Extraction Control confirms the success of the extraction step and monitors co-purification of PCR inhibitors that may cause biased or PCR false negative results. Housekeeping genes or spike in controls do not monitor the whole extraction process, especially the lysis step and in the case of housekeeping genes, extraction efficiency and degraded samples as well as the possibility of the housekeeping gene expression level changing

The primers and probe for the Extraction Controls are in the Control Mix.

The two colors are for the fluorescent dyes on the Extraction Control probe. Control Mix Red (Cy5 – emission wavelength = 670nm) and Control Mix Orange (HEX – emission wavelength = 560nm). This allows you to choose one that will fit with your existing protocol in a multiplex assay

No, the Extraction Control will work with commercially available silica-membrane extraction kits and CHELEX matrices and has been tested on a wide range of qPCR platforms.

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