qPCR Extraction Control Orange
Molecular IVD companies continue to experience tighter and tighter regulations in regard to incorporating appropriate positive and negative controls into their assays.
The qPCR Extraction Control Orange use E. coli cells of a known concentration that contain the Internal Control DNA sequence (with no known homology to any organism, so that it does not interfere with the detection of the sample target DNA). Genetic material from the test sample and the DNA extraction control are simultaneously extracted by common extraction methods, with the control being as sensitive to inhibitors and extraction failure as the test sample.
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qPCR Extraction Control Orange comprises internal control DNA sequence in E. coli cells and a control mix (containing specific primer and probe (emission wavelength = 560nm)) for qPCR detection. The cells are added to the sample and lysed and extracted with the sample DNA for downstream amplification by qPCR. The detection of the internal control DNA confirms the success of the extraction step and reduces the chance of obtaining false-negative results from the sample DNA.
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