VLP-RNA Extraction Control Red
Meridian’s VLP-RNA Extraction Controls offer a simple and effective way to reduce false-negative results in RT-qPCR assays. The biomimetic controls are composed of virus-like particle (VLP) shells packaged with a defined number of copies of target RNA molecules. Their composition closely resembles a test sample and serves as a full-process control, monitoring the success of an RT-qPCR assay from lysis/extraction to reverse transcription and amplification.
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VLP-RNA Extraction Control Red contains an internal control RNA sequence, with no known homology to any organism, encapsidated in a virus-like particle. The VLP-RNA Extraction Control is spiked in the sample prior to RNA extraction. Following RNA extraction, VLP-RNA Extraction Control can be detected in RT-qPCR adding the VLP Detection Mix (containing specific primers and probe (emission wavelength = 670nm)) to the reaction mix. The detection of VLP-RNA Extraction Control confirms the success of the extraction, reverse transcription, and amplification steps, avoiding the misinterpretation of a false negative result.
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