Lyo-Ready™ 1-Step RT-qPCR Virus Mix
Molecular diagnostic tests are progressively moving towards lyophilized. There are several advantages for this, including room temperature shipping and storage, extended shelf-life, and increased flexibility in sample volume. In order to be compatible with drying, however, enzyme preparations must be glycerol-free and include specialized excipients that preserve the mixture as it is exposed to freezing, vacuum, and dehydration. An ideal lyophilization formulation, for example, should stabilize an enzyme in a freeze-dried format and allow very fast rehydration and reactivation of the enzyme preparations, without impacting its performance post rehydration.
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Lyo-Ready™ 1-Step RT-qPCR Virus Mix is a glycerol-free formulation that contains Taq polymerase, reaction buffer, dNTP, MgCl2, lyo-excipients, and separate thermostable reverse transcriptase and dilution buffer. It is specifically optimized for amplification of RNA or DNA viruses with a high secondary structure (reverse transcriptase remains active at 55-60 °C) and is ideal for developing room temperature, lyophilized, multiplex RT-qPCR diagnostic tests.
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