Lyo-Ready 1-Step RT-qPCR Mix

Molecular diagnostic tests are progressively moving towards lyophilized. There are several advantages for this, including room temperature shipping and storage, extended shelf-life and increased flexibility in sample volume. In order to be compatible with drying however, enzyme preparations must be glycerol-free and include specialized excipients that preserve the mixture as it is exposed to freezing, vacuum and dehydration. An ideal lyophilization formulation for example, should stabilize an enzyme in a freeze-dried format and allow very fast rehydration and reactivation of the enzyme preparations, without impacting its performance post rehydration.

Can also be used wet and/or with purified nucleic acid

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Lyo-Ready 1-Step RT-qPCR Mix, MDX024

Available in 10 mL (1,000 Rxns) or 100 mL (10,000 Rxns) aliquots

Enzyme Concentrations:

Reverse transcriptase activity: 820.6 U/µL
Lot Number:
B081920, EM032-B081100, EM033-B080040, EM033-B079850, EM033-B079960, EM032-B079780, EM033-B079680, EM116-B079670

Reverse transcriptase activity: 535 U/µL
Lot Number:
EM033-B083980, EM033-B082210, EM033-B081850, EM033-B083810, EM033-B083820, EM033-B084440, EM033-B084430, EM033-B082220, EM033-B082230, EM033-B083750

Reverse transcriptase activity: 591 U/µL
Lot Number:
EM033-B099400, EM033-B098680, EM033-B087450, EM033-B088470, EM033-B090230, EM032-B084980, EM033-B093180, EM033-B092850, EM032-B092190, B0221750, EM033-B095190, EM033-B095290, EM033-B095840, EM033-B088570, EM033-B096220, EM033-B096240, EM033-B096320, EM032-B097820, EM033-B097830, EM033-B098070, B095730, EM033-B097720, EM033-B097770, EM032-B102770, EM032-B103150

Reverse transcriptase activity: 501 U/µL
Lot Number:
EM032-B090890, EM033-B090880, EM033-B104330, EM033-B104160, EM033-B105100, EM033-B106390, EM033-B107620, EM032-B107730, EM033-B107570, EM032-B107560, EM032-B110340, MDX024_EM032-B120020, EM033-B120950

Reverse transcriptase activity: 676.6 U/µL
Lot Number:
EM033-B090910, EM032-B090900, B0221760, EM033-B100340, EM033-B100490, EM033-B100900, EM033-B101440, B102820, EM033-B103530, EM033-B103860

Lyo-Ready 1-Step RT-qPCR Mix is a glycerol-free formulation that contains Taq polymerase, reaction buffer, dNTP, MgCl2, lyo-excipients, and separate thermostable reverse transcriptase and dilution buffer. It is ideal for developing room temperature, lyophilized, multiplex RT-qPCR diagnostic tests and is suited for high-throughput, automated platforms requiring milli-second rehydration times.

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