Robust PCR amplification of GC-rich human genomic DNA
A serial dilution in duplicate of human genomic DNA (1 μg, 200 ng, 100 ng, 50 ng, 25 ng and 12.5 ng, 1-6 respectively) was used with primers and Taq DNA Polymerase to amplify a 450 bp fragment of the human myc gene. The section of gene used has 61% GC content and was selected to demonstrate the high yield and sensitivity of the Taq DNA Polymerase, making it the perfect choice for the amplification of complex PCR templates.
Taq Dilution Buffer, MDX007
Taq Dilution is an optimized buffer system that has been designed to conferring long-term stability at -20 °C to high-quality Taq DNA polymerase.
Description
Taq Dilution Buffer requires the addition of a polymerase and a reaction buffer, dNTPs, and MgCl2, together with primers and templates.
Specifications
| Description | Taq HS Antibody is a mix of anti-Taq monoclonal antibodies designed to inhibit Taq DNA polymerase activity at room temperature. It is used in the preparation of hot-start PCR or qPCR master mixes and helps to prevent nonspecific amplification and primer-dimer formation, increasing specificity and sensitivity, while allowing assembly of reactions at ambient temperatures. |
| Concentration | 10 mg/mL ≤ 5% |
| Appearance | Clear, colorless solution |
| Application | PCR, multiplex PCR, qPCR, multiplex qPCR |
| Sample type | cDNA, DNA |
| Presentation | 1 vial |
| Storage | -20 °C |
| Mix stability | See outer label |
| Sensitivity | qPCR with a dilution series of human genomic DNA, to determine specific product at limiting template concentration |
| Efficiency | ± 0.5 Ct variance between test and reference sample |
| DNA Contamination | None detected in PCR amplification with traces overlay with the negative control on E. coli and mouse genomic DNA specific targets. |
产品资料
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FAQs: Taq Dilution Buffer
We generally recommend using Taq DNA Polymerase at a concentration of 25 units/mL (1.25 units/50 μL reaction). However, the optimal concentration of Taq DNA Polymerase may range from 5–50 units/mL (0.25–2.5 units/50 μL reaction) in specialized applications.
Too much Taq will result in an excessive background of unwanted DNA fragments (a smear on a gel) while a huge excess may cause the reaction to fail with no product being detected.
Due to the difficulties in pipetting small volumes of enzyme, Taq DNA Polymerase can be diluted in Taq Dilution Buffer. Unlike Taq Reaction Buffer, the Taq Dilution Buffer can then be used to store the Taq DNA polymerases, conferring long-term stability at -20 °C.
Decreasing the template concentration will help to minimize the concentration of inhibitors, the amount of DNA polymerase and primer can also be increased. In some cases you may also have to add a pre-treatment step if the DNA is not accessible.
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