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Lyo-Ready LAMP/RT-LAMP Mixes

Loop-mediated isothermal amplification (LAMP) is a DNA amplification technique that has played an increasingly important role in point-of-care (POC) diagnostics. It offers several advantages over qPCR and other amplification techniques regarding sample-to-answer time, sensitivity, specificity, cost, robustness, and accessibility, making it ideal for field-deployable diagnostics in resource-limited regions. LAMP-based assays have been used for numerous applications, including detecting pathogens such as salmonella and malaria, GM crop contamination, and forensics to detect human DNA specifically.
LAMP uses four to six primers that recognize several specific regions in the target DNA. The reaction is initiated by a strand-displacement polymerase such as Bst and two specially designed primers that form loop structures to facilitate subsequent rounds of amplification, producing high levels of DNA. LAMP reactions are very robust and inhibitor-tolerant, producing extremely large amounts of DNA that the eye can visualize (e.g., as a precipitate or color change). Specifically, 1 to 10 copies of DNA can be amplified to 109 to 1010 copies within 30 minutes, producing assays with excellent sensitivity and specificity.

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Lyo-Ready LAMP Mix, MDX097

Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots

Lyo-Ready RT-LAMP 1-Step Mix, MDX108

Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots

The Lyo-Ready LAMP Mixes are 4x master mix formulated with excipients optimized for freeze-drying to create ambient-temperature stable assays. Given cold chain management is a major limiting factor in resource-limited settings, ambient-temperature stable point-of-care (POC) assays offer several advantages, including no refrigeration, reduced shipping, and handling costs, and extended shelf-life. By using Meridian’s Lyo-Ready LAMP Mixes, the process of developing a sensitive and high-performing assay is made significantly easier, with the added flexibility of using the mix in a wet or dried-down format.

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