Bst DNA Polymerase

Bst DNA Polymerase is an enzyme derived from the large fragment of Bacillus stearothermophilus DNA Polymerase I. It contains 5´- 3´ DNA polymerase activity and strong strand displacement activity but lacks 5´- 3´ exonuclease activity. The strong strand displacement activity enables Bst DNA Polymerase to synthesize DNA at a constant temperature making it an ideal enzyme for isothermal amplification, including HDA, MCA, and Loop-Mediated Isothermal DNA Amplification (LAMP).

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Bst DNA Polymerase, MDX012

Available in 1 mL or 10 mL aliquots

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Bst DNA Polymerase (8 U/µL) is supplied with an Enzyme Dilution Buffer and a Bst Reaction Buffer formulated to deliver the fastest amplification speed (time-to-results), yield, salt tolerance, and sensitivity. For the most difficult templates, an Inhibitor-Tolerant Bst Buffer is available that is specifically designed for high performance when using crude samples such as sputum or saliva.

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