Bst DNA Polymerase
Bst DNA聚合酶由嗜硬脂芽孢杆菌中分离得到，用于等温扩增。Bst聚合酶一种具有链置换活性和5 ' -3 '聚合酶活性的耐热DNA聚合酶，但缺乏任何3 ' -5 '外切酶活性。强链置换活性使Bst DNA聚合酶能够在恒温条件下合成DNA，是环介导等温DNA扩增的理想选择。
Bst DNA Polymerase, MDX012
Available in 1 mL or 10 mL aliquots
Description
Bst DNA Polymerase (8 U/µL) is supplied with an Enzyme Dilution Buffer and a Bst Reaction Buffer formulated to deliver the fastest amplification speed (time-to-results), yield, salt tolerance, and sensitivity, speeding up your product development time and allowing you to commercialize your new products faster. For the most difficult templates, an Inhibitor-Tolerant Bst Buffer is available that is specifically designed for high performance when using crude samples such as sputum or saliva.
Specifications
| Description | DNA polymerase (exonuclease minus), with strand-displacement properties. Bst DNA Polymerase is used for Isothermal DNA amplification and LAMP (Loop-mediated Isothermal Amplification). |
| Concentration | 10 units/µL +/-10% measured against a standard |
| Appearance | Clear, colorless solution |
| Application | Loop-mediated isothermal amplification |
| Sample type | cDNA, DNA |
| Presentation | 3 vials |
| Storage | -20 °C |
| Stability | See outer label |
| Consistency | ± 0.5 Ct variance between test and reference sample |
| DNase/RNase Contamination | No detectable degradation |
产品资料
Bst DNA PolymeraseBst DNA Polymerase
FAQs: Bst DNA Polymerase
环介导等温扩增与qPCR的主要区别在于，在环介导等温扩增中，扩增是在恒温下完成的，而在qPCR中，扩增需要在一个热循环器上在多个温度之间循环。 环介导等温扩增使用具有高链置换活性的聚合酶，如Bst DNA聚合酶，由于LAMP反应不需要通过多个温度的变化，因此可以在短时间内完成快速的扩增。
No Meridian’s Bst DNA Polymerase does not come with warm or hot start; instead, the reaction buffer does not contain magnesium and so the enzyme is completely inactive until magnesium is added. If you are concerned about setting your reaction up at room temperature, we recommend adding the magnesium last, just before running the experiment and it will prevent non-specific amplification.
Yes, it is possible to design a multiplex LAMP test using different primers and probes, however they can be difficult to design and optimize as each sequence requires three sets of primers.
No, the reaction temperatures are too high for enzyme stability.
Yes, Bst DNA polymerase does have some reverse transcriptase activity, however for robust and consistent reactions we recommend the addition of a reverse transcriptase.
与我们的专业团队联系
等温扩增和qPCR反应的区别是什么?