DNA Polymerases

The ready-to-use PCR and high-fidelity PCR enzymes and DNA Polymerases have been developed for fast PCR and are designed for superior sensitivity and yield. A combination of the latest advances in buffer chemistry and PCR enhancers, in conjunction with an antibody, aptamer, or chemical-mediated polymerases and DNA Polymerases, promotes rapid, consistently accurate detection of DNA and cDNA targets. Glycerol-free options allow room temperature shipping and storage, extended shelf-life, and increased flexibility in sample volume, and isothermal options are available for loop-mediated isothermal amplification. These products are also available as components in order to fit into your assay development.
LAMP uses four to six primers that recognize several specific regions in the target DNA. The reaction is initiated by a strand-displacement polymerase such as Bst and two specially designed primers that form loop structures to facilitate subsequent rounds of amplification, producing high levels of DNA. LAMP reactions are very robust and inhibitor-tolerant, producing extremely large amounts of DNA that can be visualized by the eye (e.g., as a precipitate or color change). Specifically, 1 to 10 copies of DNA can be amplified to 109 to 1010 copies within 30 minutes, producing assays with excellent sensitivity and specificity.


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Meridian offers diagnostic manufacturers the complete solution with over 3,000 antigens and antibodies covering more than 500 diseases and an extensive range of specialty qPCR/RT-qPCR master mixes and molecular enzymes all manufactured under ISO13485 regulations.

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