Uracil DNA Glycosylase
Uracil DNA Glycosylase (UDG) is an enzyme used to eliminate carryover polymerase chain reaction (PCR) products in qPCR and RT-qPCR. This is done by incorporating dUTP in all PCR products, so any residual products from previous PCR amplifications will therefore contain dU and can be digested by UDG, preventing them from amplifying, but the fresh DNA templates will be left intact, as they will not contain dU nucleotides.
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Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases). UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation, and is inhibited by high ionic strength (>200 mM). Uracil DNA Glycosylase is tested for purity (run on SDS-PAGE gel), presence and absence of endonucleases, nickases, exonucleases, and RNases.
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