Lyo-Ready™ Direct DNA and RNA/DNA LAMP Urine Master Mixes
Lyo-Ready™ 可冻干尿液直扩LAMP/RT-LAMP预混液是即用型、4x浓度、完全化的配方，适用于对尿液样本的直接等温扩增 （LAMP），可显著提高研发速度和出结果时间。预混液不含甘油，含有优化的冻干赋形剂，可以用于开发常温稳定的检测，是用于POC尿液检测的理想选择。
Can also be used wet and/or with purified nucleic acid
Lyo-Ready™ Direct DNA LAMP Urine, MDX154
Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots
Lyo-Ready™ Direct RNA/DNA LAMP Urine, MDX155
Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots
Urinary tract infections (UTIs) affect over 150 million people worldwide and are the second most common cause of infection in primary and secondary care and most women experience at least one episode of acute uncomplicated cystitis in their lifetime. UTIs are responsible for a major part of antibiotic prescriptions, accounting for up to 15% of antimicrobial use in the community, with antibiotic use been described as one of the main factors contributing to the emergence of antimicrobial resistance. With the stigma attached to sexually transmitted diseases (STDs), and with their rapid spread in the last few years, there is also a need for discrete, fast and accurate point-of-care (POC) tests as well.
The Lyo-Ready™ Direct DNA LAMP Urine and Lyo-Ready™ RNA/DNA LAMP Urine mixes combine even greater tolerance with fast, robust isothermal amplification to enable highly sensitive direct detection using crude urine samples. The mixes are uniquely designed to overcome much higher concentrations of inhibitors and so do not require a urine DNA or urine RNA purification step analyse a patient sample. Very fast reaction kinetics result in faster time to results and superior amplification sensitivity and specificity. In addition, the 4x concentrated master mixes are formulated with lyo-excipients which are optimized for freeze-drying to create ambient-temperature stable assays, making them ideal for POC urine testing.
产品资料
可冻干尿液直扩LAMP预混液可冻干尿液直扩LAMP预混液
可冻干LAMP试剂可冻干LAMP试剂
可冻干RT-LAMP试剂可冻干RT-LAMP试剂
Air-Dryable Direct Urine CatalogAir-Dryable Direct Urine Catalog
FAQs: LAMP Urine Test
环介导等温扩增与qPCR的主要区别在于，在环介导等温扩增中，扩增是在恒温下完成的，而在qPCR中，扩增需要在一个热循环器上在多个温度之间循环。 环介导等温扩增使用具有高链置换活性的聚合酶，如Bst DNA聚合酶，由于LAMP反应不需要通过多个温度的变化，因此可以在短时间内完成快速的扩增。
The advantages of an isothermal amplification urine test is that it is completely non-invasive, and it can be self-sampled in large quantities. Urine testing is used in the diagnosis and management of infectious diseases (including STDs) and urinary tract infections (UTIs).
The most common infections are urinary tract infections (UTIs), most commonly E. coli, P. mirabilis, Enterococcus, Staphylococcus and Klebsiella. This is followed by sexually transmitted diseases (STDs) such as syphilis, gonorrhoea, chlamydia, trichomoniasis, hepatitis B (HBV), herpes simplex virus (HSV), HIV and human papillomavirus (HPV). Several other infections can be diagnosed by urine DNA and urine RNA tests including community-acquired pneumonia (CAP), legionellosis, tuberculosis, congenital cytomegalovirus (CMV) infection, and arboviruses, such as Chikungunya virus, dengue virus and Zika virus. Parasites can also be diagnosed from urine testing for the detection of eggs from Schistosoma haematobium.
We have used up to 30% human urine with our mix.
冻干后的预混液在正确封存的情况下可在常温下稳定保持2年。
That will depend on the bacteria, for some bacteria you may not need to do any pre-treatment, however some may require heating to 95°C for 5 min and for some tougher bacteria, a Proteinase K step (Proteinase K 55°C for 15 min and then at 98°C for 5 min) or using a non-ionic detergent (such as Tween-20 to final concentration of 0.2% followed by incubation at room temperature for 10 min) may be necessary.
We do not see a performance difference between the two mixes, which is why we call the Lyo-Ready Direct RT-LAMP Urine mix – Lyo-Ready™ Direct RNA/DNA LAMP Urine mix, as it can be used for both.
是的，Lyo-Ready™ Direct DNA LAMP Urine and Lyo-Ready™ Direct RNA/DNA LAMP Urine can be used as a liquid mix or lyophilization and stored for up to 24 months without needing to change the reaction conditions and lyophilization/ambient temperature storage will not affect the sensitivity of the test.
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