LAMP Master Mixes
Bst. Enzymes & Buffers
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High-Sensitivity LAMP Master Mixes for Rapid POCT Development
- Inhibitor-tolerant isothermal mix designed for direct isothermal amplification from crude samples.
- Very fast reaction kinetics – ideal for point of care testing.
- Fully optimized, just add custom primers and probes.
LAMP master mixes enabling highly sensitive detection of target DNA or RNA. Meridian has also developed sample specific inhibitor tolerant LAMP mixes, designed to be used for direct amplification from crude lysates or inhibitor-rich samples, with the ability to tolerate even higher levels of inhibitors present in samples. In addition, these mixes can be used as wet mixes or dried by lyophilization or air-drying for room temperature shipping and storage, extended shelf-life, and increased flexibility in sample volume, making them ideal for POCT development where minimal sample processing and fast turn-around times are essential.
产品资料
Lyo-Ready LAMP Master Mix Lyo-Ready LAMP Master Mix
Lyo-Ready Direct DNA LAMP Blood Master Mixes Lyo-Ready Direct DNA LAMP Blood Master Mixes
Lyo-Ready Direct Stool Urine LAMP Master Mixes Lyo-Ready Direct Stool LAMP Master Mixes
Specimen-specific and Universal qPCR & LAMP Master Mixes Specimen-specific and Universal qPCR & LAMP Master Mixes
Lyo-Ready RT-LAMP 1-Step Master Mix Lyo-Ready RT-LAMP 1-Step Master Mix
Lyo-Ready Direct LAMP Saliva Master Mixes Lyo-Ready Direct LAMP Saliva Master Mixes
Lyo-Ready Direct LAMP Urine Master Mixes Lyo-Ready Direct LAMP Urine Master Mixes
环介导等温扩增与qPCR的主要区别在于，在环介导等温扩增中，扩增是在恒温下完成的，而在qPCR中，扩增需要在一个热循环器上在多个温度之间循环。 环介导等温扩增使用具有高链置换活性的聚合酶，如Bst DNA聚合酶，由于LAMP反应不需要通过多个温度的变化，因此可以在短时间内完成快速的扩增。
Yes, it is possible to design a multiplex LAMP test using different primers and probes, however they can be difficult to design and optimize as each sequence requires three sets of primers.
We do not see a performance difference between the two mixes, which is why we call the Lyo-Ready Direct RT-LAMP Blood mix – Lyo-Ready Direct RNA/DNA LAMP Blood mix, as it can be used for both.
Yes, the LAMP and RT-LAMP master mixes can be used as a liquid mix or lyophilized and stored for up to 24 months without needing to change the reaction conditions and this drying/ambient temperature storage will not affect the sensitivity of the POCT.
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