Close

Air-Dryable Direct DNA and RNA/DNA qPCR Blood Master Mixes

Air-Dryable Direct DNA qPCR and RNA/DNA qPCR Blood mixes are glycerol-free mixes that contain optimized excipients compatible with air and oven drying and they are designed for the direct molecular quantitation of DNA and RNA from whole blood, serum, or plasma.

Can also be used wet and/or with purified nucleic acid

订购样品

对产品有疑问吗?

欢迎联系我们了解更多迈迪安分子和免疫的产品系列!

联系专家团队

Meridian's Air-Dryable Direct DNA and RNA/DNA qPCR Blood Master Mixes

  • Create ultra-sensitive multiplex direct blood qPCR tests using whole blood, serum or plasma.

  • Simplify the development of quantitative PCR blood tests, by removing the nucleic acid extraction step and shortening the sample-to-result workflows.

  • Validated for detection of blood pathogens and liquid biopsy applications.

  • Can be used wet or air-dried for developing ambient-temperature stable assays.

Air-Dryable Direct DNA and RNA/DNA qPCR Blood graph

Air-Dryable Direct DNA qPCR Blood, 4x, MDX092

Application: qPCR Specimen Type: Blood, Plasma, Serum,Air-Dryable Mix Concentration: 4x High Concentration: Yes Wet: Yes
Glycerol-Free: Yes Lyo-Ready: No Air-Dryable: Yes Dryable: Air-Dryable Sustainability: Air-Dryable

Inhibitor tolerance to 20% whole blood, 20% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.

Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Air-Dryable Direct RNA/DNA qPCR Blood, 4x, MDX121

Application: RT-qPCR Specimen Type: Blood, Plasma, Serum, Air-Dryable Mix Concentration: 4x High Concentration: Yes Wet: Yes
Glycerol-Free: Yes Lyo-Ready: No Air-Dryable: Yes Dryable: Air-Dryable Sustainability: Air-Dryable

Inhibitor tolerance to 10% whole blood, 10% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.

Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Blood is one of the most common specimens used for laboratory diagnostic testing and it is useful for evaluating the function of vital organs (kidneys, liver, thyroid, and heart) and for diagnosing diseases such as bacterial and viral infections, cancer, cardiovascular disease, and metabolic disorders such as diabetes. However, whole blood specimens, serum, and plasma contain a number of inherent PCR inhibitors including immunoglobulin G, hemoglobin, lactoferrin, and leukocyte DNA. In addition, PCR inhibitors can be found in the anticoagulants used to stabilize blood samples (e.g. EDTA, citrate, or heparin). Traditional methods have relied on removing these inhibitors by DNA or RNA extraction prior to testing, however, these methods are problematic, can cause sample loss, and are not 100% effective at removing all the inhibitors.

Air-Dryable DNA qPCR Blood is a 4x qPCR master mix for the development of DNA blood tests, enabling highly sensitive detection of target DNA from whole blood, plasma, and serum samples. Air-Dryable RNA/DNA qPCR Saliva is a 4x qPCR master mix and can be used for the development of both DNA and RNA blood tests from whole blood, plasma, and serum samples. These mixes have been formulated specifically to overcome the inhibitors found in blood samples – no further optimization is required aside from the addition of primers and probes. Furthermore, these mixes can be used in a wet format or oven dried to create ambient-temperature stable whole blood tests, plasma tests or serum tests.

FAQs: qPCR Blood Test

Blood contains abundant information about the health status of the organism, it can be used to evaluate organ function, detect proteins, nucleic acids, metabolites, infectious diseases and can be used for diagnosis of cancer (liquid biopsy), heart disease, diabetes. DNA and RNA Blood tests are a simple and easily accessible (low-invasive) method to assess and monitor health.

Immunoglobulin G is the major inhibitor of qPCR in blood, as it can bind to single-stranded genomic DNA, leading to increased Ct values in a quantitative PCR blood test. The other two main inhibitors in blood are hemoglobin and lactoferrin that affect the DNA polymerase activity and thus lower the amplification efficiency. Hemoglobin and hematin can also cause some fluorescence quenching, particularly for FAM, although this should not affect the Ct value. In addition, inhibitors can be found in the anticoagulants used to stabilize blood samples (e.g. EDTA, citrate, or heparin).

We have used up to 20% whole blood with our mix, higher concentrations cause to solution to become viscous and difficult to work with.

We use our own proprietary Taq polymerase and a highly optimized buffer system to overcome inhibition.

冻干后的预混液在正确封存的情况下可在常温下稳定保持2年。

Yes, the Air-Dryable qPCR Blood kits can be used to detect both cfDNA and cfRNA, making it ideal for liquid biopsy.

Yes, it is possible that you may need to pre-treat the blood in order to release the DNA from the gram-positive bacteria. In some cases a heat shock is sufficient, this will depend on the bacteria being examined.

We do not see a performance difference between the two mixes, which is why we call the Air-Dryable Direct RT-qPCR Blood mix – Air-Dryable Direct RNA/DNA qPCR Blood mix, as it can be used for both.

It can be stored frozen or following the instructions from the storage buffer manufacturer.

They are compatible with standard cycling conditions and can also be used with very fast cycling conditions, however some optimization may be required for the amount of blood that is needed for qPCR blood tests.

You should elute in water, or TE buffer.

Yes, the Air-Dryable Direct DNA qPCR Blood and Air-Dryable Direct RNA/DNA qPCR Blood can be used as a liquid mix or air/oven dried and stored for up to 24 months without needing to change the reaction conditions and air drying/ambient temperature storage will not affect the sensitivity of the test.

We advise not using Triton; however, heat treatment is ok.

与我们的专业团队联系

想了解更多迈迪安免疫和分子产品信息?欢迎与我们联系


By submitting your information in this form, you agree that your personal information may be stored and processed in any country where we have facilities or service providers, and by using our “Contact Us” page you agree to the transfer of information to countries outside of your country of residence, including to the United States, which may provide for different data protection rules than in your country. The information you submit will be governed by our Privacy Statement.
公司简介
了解更多
Welcome to
logo blue

Please select your country to continue

Continue
EN