Air-Dryable Master Mixes
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Advanced Air-Dryable Master Mixes for High-Sensitivity Assays
Meridian’s air-dryable master mixes are the market-leading solution for molecular diagnostic assays requiring fast cycling, superior sensitivity, high inhibitor tolerance and ambient temperature stability.
- High efficiency and sensitivity in multiplex reactions, just add primers, probes and sample.
- Available in universal or specimen-specific formulations for blood, saliva, urine or stool and suitable for direct detection assays using crude samples.
- Can be used in a liquid or dry format, reducing the cost and complexity of creating ambient-temperature stable assays.
Meridian master mixes combining the lasts advances in buffer chemistry and PCR enhancers, together with an optimized concentration of enzymes, dNTPs and MgCl2. and air-dry compatible excipients. They come as standard as well as specimen-specific qPCR and RT-qPCR master mixes, developed for highly reproducible, accurate RNA and DNA target amplification, delivering excellent results in multiplex assays, with sensitivity down to a single copy.
- Air-Dryable™ Direct DNA and RNA/DNA qPCR Blood Master Mixes
- Air-Dryable™ Direct DNA and RNA/DNA qPCR Saliva Master Mixes
- Air-Dryable™ Direct DNA and RNA/DNA qPCR Stool Master Mixes
- Air-Dryable™ Direct DNA and RNA/DNA qPCR Urine Master Mixes
- Air-Dryable™ Direct DNA qPCR Plant Master Mix
- Air-Dryable™ qPCR and RT-qPCR Mixes Master Mixes
产品资料
Air-Dryable Master MixesAir-Dryable Master Mixes
Air-Dryable Direct Blood Master MixesAir-Dryable Direct Blood Master Mixes
可风干唾液直扩可风干唾液直扩
用于直扩粪便样本的可风干预混液用于直扩粪便样本的可风干预混液
可风干尿液直扩可风干尿液直扩
Air-Dryable Direct Plant Master MixesAir-Dryable Direct Plant Master Mixes
可风干分子检测快速使用指南可风干分子检测快速使用指南
相关产品
Lyophilization is done by freezing and sublimation in a vacuum, forming a cake or bead, it has very low viscosity and up to 99% of the moisture is removed. Drying normally take 18-24 hours and requires cryo-excipients that protect the enzymes during the freezing. The cost is high as a freeze vacuum drier and low moisture environment are required.
Air-drying is done by heating in a convection oven, forming a highly viscous pellet, with about 95% of the moisture removed. Drying normally take less than 2 hours and requires heat protecting excipients that protect the enzymes at higher temperatures. The cost is low as no additional equipment is required.
The Air-Dryable™ qPCR and RT-qPCR master mixes have some inhibitor tolerance, but for amplification from crude lysates or inhibitor-rich samples we have the air-dryable sample specific (blood, saliva, urine, stool, and plant) mixes.
Yes, reaction conditions are the same and they will produce the same Ct values, even with multiplex qPCR assays. This means that the liquid mixes can be used to create SOPs if you do not want to dry down and then later if drying is required (for example to increase sensitivity), the SOP can be updated for oven drying, it does not require completely new SOPs to be written.
冻干后的预混液在正确封存的情况下可在常温下稳定保持2年。
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