Immunoassay interference can cause false-positive and false-negative test results, leading to misinterpretation and improper patient diagnosis. The most common causes of interference are due to Human Anti-Mouse Antibodies (HAMA), Rheumatoid Factor (RF), and Heterophilic Antibodies (HA). HAMA are the most prevalent and are identified in up to 25% of the patient population. Combined with HA, they represent the most significant factors leading to false positive immunoassay results.
Avoiding immunoassay interference requires blockers to be included in every immunoassay. Historically, animal-based interference blockers have been used to improve assay accuracy; however, they are susceptible to cross-reactivity with assay reagents, lot-to-lot variability, and supply shortages – as seen during the COVID-19 pandemic. Meridian’s new animal-free commercial blockers, K-BlockTM and Mouse-FREE IgG, are the next-generation solution for blocking HAMA/RF/HA that overcomes the ethical concerns and supply problems in using animal-derived blockers.
In this webinar you will learn the advantages of using animal-free immunoassay blockers and their performance.
Ryan Hughes Ph.D., (Life Science Immunology Product Manager, Meridian Bioscience)
Andy Gaspar Ph.D., (Life Science Technical Operations and Development Manager, Meridian Bioscience)
Steve Hawkins, PhD (Product Marketing Manager, Meridian Bioscience)