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Glycerol-Free T4 Polynucleotide Kinase (HC)

T4 Polynucleotide Kinase is widely used to phosphorylate the 5’ end of both DNA and RNA during labeling and ligation by catalyzing the transfer of the γ-phosphate from ATP to the 5´ hydroxyl of single stranded and double stranded DNA, RNA, and nucleoside 3´ monophosphates. T4 Polynucleotide Kinase also catalyzes the hydrolytic removal of the 3´ phosphate from 3´-phosphoryl polynucleotides, deoxyribonucleoside 3´ monophosphates, and deoxyribonucleoside-3´,5´-diphosphates to form a 3´-hydroxyl group.

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Meridian's Glycerol-Free T4 Polynucleotide Kinase (HC)

  • 5' phosphorylation for subsequent ligation for NGS

  • End-labeling for probes and oligodeoxynucleotide primers

  • Removes 3′ phosphoryl groups from DNA and RNA

  • Glycerol-free, for lyophilization and ambient temperature storage

Glycerol-Free T4 Polynucleotide Kinase, MDX206

Application: NGS Specimen Type: NGS Enzymes Concentration: 10 U/µL High Concentration: No Wet: Yes
Glycerol-Free: YesYes Lyo-Ready: Requires excipients Air-Dryable: Requires excipients Dryable: Requires excipients Sustainability: Glycerol-Free

Glycerol-free enzyme used to phosphorylate the ends of both DNA and RNA during labeling and ligation, mainly used in next-generation sequencing (NGS) library preparation. Ideal for lyophilization.

Available in 50 µL (500 Units) or 500 µL (5,000 Units) aliquots

Glycerol-Free T4 Polynucleotide Kinase (HC) is supplied at 20 U/L and is mainly used in next-generation sequencing (NGS) library preparation, where it can be used separately or as part of an End-Repair Mix, where phosphates are added to the 5′-hydroxyl terminus of double stranded DNA for subsequent ligation of adaptors by T4 DNA Ligase.
Glycerol-Free T4 Polynucleotide Kinase (HC) is in a glycerol-free storage buffer and is supplied with a Lyo-Ready reaction buffer and has been optimized to deliver excellent, stable performance without glycerol, allowing you to deliver reliable results with the added capability for lyophilization. This gives you the flexibility to confidently store mixtures at ambient temperature or produce diagnostic assays that rely on miniaturized reaction components, such as point-of-care NGS.

FAQs: Glycerol-Free T4 Polynucleotide Kinase

Yes, but not for radioactive labelling reactions as the non-radioactive ATP from the T4 Ligase buffer will interfere.

Yes, incubation for 20 minutes at 65°C completely inactivates T4 Polynucleotide Kinase.

High-concentration enzymes help reduce volumes when drying down, making the process faster, they also reduce volumes in microfluidic, cartridges and point of care (POC) devices, allowing for more sample to be added. They facilitate optimization and can speed up the reaction (as long as there is substrate available to bind to), increasing sensitivity and yield, as a result, they can be employed in a wide range of assay formats, allowing more flexibility for development and cost saving during commercialization.

High-concentration enzymes help reduce volumes when drying down, making the process faster, they also reduce volumes in microfluidic, cartridges and point of care (POC) devices, allowing for more sample to be added. They facilitate optimization and can speed up the reaction (as long as there is substrate available to bind to), increasing sensitivity and yield, as a result, they can be employed in a wide range of assay formats, allowing more flexibility for development and cost saving during commercialization.

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Have questions about a product? Want to learn more about Meridian’s molecular or immunoassay reagent portfolio? We want to hear from you!


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