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PCR Enzymes

We offer a selection of high-quality enzymes help you develop highly sensitive assays.
Ready-to-use PCR and high-fidelity PCR enzymes and DNA polymerases have been developed for fast PCR and are designed for superior sensitivity and yield. A combination of the latest advances in buffer chemistry and PCR enhancers, in conjunction with an antibody, aptamer, or chemical-mediated polymerases and DNA polymerases, promotes rapid, consistently accurate detection of DNA and cDNA targets. Glycerol-free options allow room temperature shipping and storage, extended shelf-life, and increased flexibility in sample volume, and isothermal options are available for loop-mediated isothermal amplification (LAMP).
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Name Specimen Type Application Catalog Order a Sample Sizes Description Wet(Liquid) Air-Dryable Dryable Glycerol-Free Lyo-Ready Concentration High Concentration Detail Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Glycerol-Free DNA Pol I Klenow Fragment (HC) NGS Enzymes NGS MDX208 Order a Sample Available in 200 µL (10,000 Units) or 1 mL (50,000 Units) aliquots

Glycerol-free, high concentration DNA Pol I Klenow Fragment, designed for DNA blunting by 3’ overhang removal and fill-in of 5’ overhangs for adapter ligation in next generation sequencing library preparation. Ideal for lyophilization.

Yes Requires excipients Requires excipients YesYes Requires excipients 50 U/µL Yes
Glycerol-Free T4 DNA Polymerase (HC) NGS Enzymes NGS MDX207 Order a Sample Available in 50 µL (500 Units) or 500 µL (5,000 Units) aliquots

Glycerol-free, high concentration DNA polymerase used for generating blunt ends on any duplex DNA molecule, used in next-generation sequencing (NGS) library preparation. Ideal for lyophilization.

Yes Requires excipients Requires excipients YesYes Requires excipients 10 U/µL Yes
Glycerol-Free T4 Polynucleotide Kinase NGS Enzymes NGS MDX206 Order a Sample Available in 50 µL (500 Units) or 500 µL (5,000 Units) aliquots

Glycerol-free enzyme used to phosphorylate the ends of both DNA and RNA during labeling and ligation, mainly used in next-generation sequencing (NGS) library preparation. Ideal for lyophilization.

Yes Requires excipients Requires excipients YesYes Requires excipients 10 U/µL No
Glycerol-Free HS Tth DNA Polymerase (HC) DNA Polymerase, Reverse Transcriptase RT-qPCR MDX205 Order a Sample Available in 50 µL (4,000 Unit) or 500 µL (100,000 Unit) aliquots

Glycerol-free, high concentration, thermostable DNA polymerase with intrinsic reverse transcriptase (RT) activity and unlike other RT enzymes, it is not associated with RNase H activity. Ideal for lyophilization.

Yes Requires excipients Requires excipients YesYes Requires excipients 200 U/µL Yes
Glycerol-Free High-Fidelity Pfu (HC) DNA Polymerase, NGS Enzymes PCR MDX203 Order a Sample Available in 0.025 mL (500 Units) or 1 mL (20,000 Units) aliquots

Glycerol-free, high concentration, high fidelity DNA polymerase with separate dilution and reaction buffer, ideal for NGS library amplification and gene expression. Ideal for lyophilization.

Yes Requires excipients Requires excipients YesYes Requires excipients 20 U/µL Yes
Glycerol-Free RNase H (HC) Reverse Transcriptase, NGS Enzymes, NASBA RT-qPCR MDX202 Order a Sample Available in 100 µL (2,500 Unit) or 250 µL (12,500 Unit) aliquots

Glycerol-free, high concentration RNA-specific endonuclease that hydrolyzes the phosphodiester bonds of RNA, when hybridized to DNA. Ideal for lyophilization.

Yes Requires excipients Requires excipients YesYes Requires excipients 50 U/µL Yes
Glycerol-Free T7 RNA Polymerase (HC) NASBA NASBA MDX201 Order a Sample Available in 50,000 Unit or 500,000 Unit aliquots

Glycerol-free, high concentration DNA-dependant RNA Polymerase that requires a T7 promoter sequence, it an ideal enzyme for in-vitro synthesis of labeled or unlabeled RNA for research, diagnostic or clinical applications. Ideal for lyophilization.

Yes Requires excipients Requires excipients YesYes Requires excipients 1,000 U/µL Yes
Gycerol-free T4 DNA Ligase (HC) NGS Enzymes NGS MDX200 Order a Sample Available in 1,000 Unit or 10,000 Unit aliquots

Glycerol-free, high concentration enzyme that efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. Ideal for lyophilization.

Yes Requires excipients Requires excipients YesYes Requires excipients 50 Weiss unit/µL Yes
Glycerol-Free Taq HS High Conc. DNA Polymerase DNA Polymerase, NGS Enzymes PCR MDX170 Order a Sample Available in 0.025 mL (1,000 Units) or 0.5 mL (25,000 Units) aliquots

Glycerol-free, high concentration, high fidelity DNA polymerase with separate dilution and reaction buffer, ideal for NGS library amplification and gene expression. Ideal for lyophilization.

Yes Requires excipients Requires excipients YesYes Requires excipients 50 U/µL Yes
55C MMLV-RT Reverse Transcriptase, Air-Dryable & Lyo-Ready Enzymes RT-LAMP MDX117 Order a Sample Available in 50 µL (10,000 Units), 200 µL (40,000 Units) or 1 mL (200,000 Units) aliquot

Highly robust, thermostable reverse transcriptase, ideal for cDNA synthesis from RNA with a high secondary structure such as viral RNA genomes.

Yes Requires excipients Requires excipients YesYes Requires excipients 200 U/µL Yes
Uracil DNA Glycosylase Others qPCR MDX054 Order a Sample Available in 10 mL (10,000 Rxns) aliquots

Used to eliminate carryover polymerase chain reaction (PCR) products in qPCR and RT-qPCR,by incorporating dUTP in reactions, so residual products from previous PCR amplifications containing dUs can be digested.

Yes No No NoNo No 1 U/µL No
Proteinase K Solution Others Extraction MDX055 Order a Sample Available in 25 mL aliquots

Proteinase K Solution is an enzyme used to digest most proteins in molecular biological techniques.

Yes No No NoNo No 20 mg/mL No
MMLV-RT Reverse Transcriptase RT-qPCR MDX044 Order a Sample Available in 200 µL (1,000 Rxns) or 2 mL (10,000 Rxns) aliquots

For cDNA synthesis and subsequent PCR or qPCR in a one-step or two-step assay.

Yes No No NoNo No 100x No
RNase-Tolerant MMLV-RT Reverse Transcriptase RT-qPCR MDX043 Order a Sample Available in 200 µL (1,000 Rxns) or 2 mL (10,000 Rxns) aliquots

For the detection of RNA at very low levels, it can also be used with a broader temperature range, making it ideal for applications such as blood bank or transplant viral testing.

Yes No No NoNo No 100x No
Lyo-Compatible MMLV-RT Reverse Transcriptase, Air-Dryable & Lyo-Ready Enzymes RT-qPCR MDX042 Order a Sample Available in 8 µL (1,000 Rxns) or 80 µL (10,000 Rxns) aliquots

Glycerol-free high concentration reverse transcriptase and dilution buffer. Can be used to create 1-step RT-qPCR mixes for subsequent lyophilization.

Yes Requires excipients Requires excipients YesYes Requires excipients > 165 U/µL Yes
Taq DNA Polymerase DNA Polymerase PCR MDX001 Order a Sample Available in 0.5 mL (500 Units) or 10 mL (50,000 Units) aliquots

Very high yield, high-performance polymerase, to give fast, superior amplification, even with challenging templates and in the presence of PCR inhibitors.

Yes No No NoNo No 5 U/mL No
High Conc. Glycerol-Free Bst Isothermal DNA Polymerase, Bst Enzymes & Buffers LAMP MDX018 Order a Sample Available in 80 µL or 800 µL aliquots

High concentration glycerol-free DNA polymerase (exo-), with strand-displacement properties. used for Isothermal DNA amplification such as LAMP (Loop-mediated Isothermal Amplification).

Yes Requires excipients Requires excipients YesYes Requires excipients 100 units/mL Yes
Aptamer Taq HS (Glycerol-Free) DNA Polymerase, Air-Dryable & Lyo-Ready Enzymes PCR MDX015 Order a Sample Available in 20 μL (1,000 Units) or 1 mL (50,000 Units) aliquots

Glycerol-free, high concentration hot-start Taq DNA polymerase, suitable for difficult, multiplex molecular tests that require fast amplification.

Yes Requires excipients Requires excipients YesYes Requires excipients 50 U/mL Yes
Taq HS Antibody Others PCR MDX014 Order a Sample Available in 20 µL (1,000 Units) or 250 µL (12,500 Units) aliquots

Mix of anti-Taq monoclonal antibodies designed to block Taq DNA polymerase activity at room temperature. Used in the preparation of hot-start PCR or qPCR master mixes.

Yes Requires excipients Requires excipients YesYes Requires excipients 50 U/mL Yes
Bst DNA Polymerase Isothermal DNA Polymerase, Bst Enzymes & Buffers LAMP MDX012 Order a Sample Available in 1 mL or 10 mL aliquots

DNA polymerase (exo-), with strand-displacement properties. used for Isothermal DNA amplification such as LAMP (Loop-mediated Isothermal Amplification).

Yes No No NoNo No 8 units/mL No
Glycerol-Free Taq HS, 50 U/µL DNA Polymerase, Air-Dryable & Lyo-Ready Enzymes PCR MDX011 Order a Sample Available in 0.02 mL (1,000 Units) or 0.5 mL (25,000 Units) aliquots

Lyophilization-compatible high-performance Taq DNA polymerase, separate Taq antibody and enzyme dilution buffer, suitable for difficult, multiplex tests that require fast amplification, whilst maintaining high specificity and sensitivity.

Yes Requires excipients Requires excipients YesYes Requires excipients 50 U/mL Yes
Low DNA Taq HS 5 U/µL DNA Polymerase PCR MDX009 Order a Sample Available in 0.1 mL (500 Units) or 10 mL (50,000 Units) aliquots

Highly purified Taq DNA polymerase with low DNA background and stringent chemical hot-start properties, ideal for PCR of low-copy bacterial targets to avoid false-positive amplification, such as in water testing.

Yes No No NoNo No 5 U/mL No
Taq HS DNA Polymerase DNA Polymerase PCR MDX008 Order a Sample Available in 0.1 mL (500 Units) or 10 mL (50,000 Units) aliquots

Very high yield, high-performance, antibody-mediated hot-start polymerase, to give fast, superior amplification, even with challenging templates and in the presence of PCR inhibitors.

Yes No No NoNo No 5 U/mL No
High-Fidelity Pfu DNA Polymerase, NGS Enzymes PCR MDX003 Order a Sample Available in 2 mL (500 Units) or 10 mL (25,000 Units) aliquots

Thermostable high fidelity Hot Start DNA polymerase, generates blunt-ended amplicons up to 5 kb in length making it ideal for high yields in NGS library amplification.

Yes No No NoNo No 2 U/mL No

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Ready-to-Use PCR and High-Fidelity PCR Enzymes

Meridian also as a selection of reverse transcriptases for cDNA synthesis, including a reverse transcriptase for dealing with RNA that contains high amounts of secondary structure, such as RNA viruses and reverse transcriptase for dealing with crude samples, particularly in RT-PCR and RT-qPCR applications for the detection of very low-copy targets. We also have a high concentration lyophilization ready option, for incorporation into lyophilized RT-qPCR assays for room temperature shipping and storage, extending shelf-life and increased flexibility in sample volume.

We also have RNases and DNases free Proteinase K that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of native RNA and DNA from tissues or cell lines and it is especially suitable for isolating qPCR and RT-qPCR templates.

Core Enzymes for Nucleic Acid Processes

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Why We Are Second To None

Meridian offers diagnostic manufacturers the complete solution with over 3,000 antigens and antibodies covering more than 500 diseases and an extensive range of specialty qPCR/RT-qPCR master mixes and molecular enzymes all manufactured under ISO13485 regulations.

  • Path Copy

    Large Sample Size

  • Lot Reservation

    Lot Reservation

  • ISO13485

    ISO13485

  • World-Class Customer Service

    World-Class Customer Service

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Have questions about a product? Want to learn more about Meridian’s molecular or immunoassay reagent portfolio? We want to hear from you!


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