Glycerol-Free T4 DNA Polymerase (HC) is supplied at 10 U/µL and catalyzes the addition of nucleotides to the 3′ end of a DNA strand, extending it in the 5′ to 3′ direction. It also possesses 3′ to 5′ exonuclease activity, allowing it to remove nucleotides from the 3′ end of DNA strands. This activity is useful in various DNA manipulation techniques, such as DNA sequencing, site-directed mutagenesis, and DNA repair studies. In addition, it has 5′ to 3′ exonuclease activity, enabling it to remove nucleotides from the 5′ end of DNA molecules.

It is used in next-generation sequencing (NGS) library preparation, where it can be used separately or as part of an End-Repair Mix, where it can blunt DNA ends of double-stranded DNA fragments for subsequent ligation of adaptors by T4 DNA Ligase.

Glycerol-Free T4 DNA Polymerase (HC) is in a glycerol-free storage buffer and is supplied with a reaction buffer and has been optimized to deliver excellent, stable performance without glycerol, allowing you to deliver reliable results with the added capability for lyophilization. This gives you the flexibility to confidently store mixtures at ambient temperature or produce diagnostic assays that rely on miniaturized reaction components, such as point-of-care NGS.