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特定样本类型 Specimen-specific 抗抑性预混液

临床和环境样品中固有的各种不同抑制剂会对分子检测的敏感性和准确性产生负面影响。为了克服这一问题,传统检测方法中样品在检验之前要进行预处理加工。然而,提取技术非100%有效性可能会影响可用于检测的核酸靶标的数量,一些抑制剂可以在纯化后协同洗脱,导致假阴性检测结果。 迈迪安公司推出的抗抑性qPCR/RT-qPCR预混液可用于从粗裂解物或富含抑制物的样本(如痰液、唾液、尿液、粪便或血液)中直接进行免核酸提取扩增。适用于要求快速周转时间或最小样品处理以防止样品丢失的定量分析。
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姓名 Specimen Type Application   Order a Sample Sizes Description Wet(Liquid) Air-Dryable Dryable Glycerol-Free Lyo-Ready Concentration High Concentration Detail Safety Data Sheet COA/Test Release 使用手册 产品指南
Lyo-Ready Genotyping Direct qPCR FFPE, 4x FFPE, Lyo-Ready Mix SNP Genotyping MDX168 Order a Sample Available in 1,000 Unit or 10,000 Unit aliquots

Better extraction buffers and greater tolerance to crude FFPE samples. Compatible with all dual-label probe chemistries for detection of genetic variants, such as single nucleotide and copy number variants, to give highly reproducible allelic discrimination.

Yes No Lyo-Ready Yes Yes 4x Yes
Lyo-Ready Genotyping Direct qPCR Urine, 4x Urine, Lyo-Ready Mix SNP Genotyping MDX158 Order a Sample Available in 1,000 Unit or 10,000 Unit aliquots

Inhibitor tolerance to 20% urine. Compatible with all dual-label probe chemistries for detection of genetic variants, such as single nucleotide and copy number variants, to give highly reproducible allelic discrimination.

Yes No Lyo-Ready Yes Yes 4x Yes
Lyo-Ready Direct RNA/DNA LAMP Urine, 4x Urine, LAMP Mixes RT-LAMP MDX155 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.

Yes No Lyo-Ready Yes Yes 4x Yes
Lyo-Ready Direct DNA LAMP Urine, 4x Urine, LAMP Mixes LAMP MDX154 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.

Yes No Lyo-Ready Yes Yes 4x Yes
Air-Dryable Direct DNA qPCR Stool, 4x Stool, Air-Dryable Mix qPCR MDX140 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.

Yes Yes Air-Dryable Yes No 4x Yes
Lyo-Ready Direct RNA/DNA qPCR Urine, 4x Urine, Lyo-Ready Mix RT-qPCR MDX153 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.

Yes No Lyo-Ready Yes Yes 4x Yes
Lyo-Ready Direct DNA qPCR Urine, 4x Urine, Lyo-Ready Mix qPCR MDX152 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.

Yes No Lyo-Ready Yes Yes 4x Yes
Air-Dryable Direct RNA/DNA qPCR Urine, 4x Urine, Air-Dryable Mix RT-qPCR MDX151 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.

Yes Yes Air-Dryable Yes No 4x Yes
Air-Dryable Direct DNA qPCR Urine, 4x Urine, Air-Dryable Mix qPCR MDX150 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 60% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.

Yes Yes Air-Dryable Yes No 4x Yes
Lyo-Ready Genotyping Direct qPCR Stool, 4x Stool, Lyo-Ready Mix SNP Genotyping MDX148 Order a Sample Available in 1,000 Unit or 10,000 Unit aliquots

Inhibitor tolerance to 20% stool. Compatible with all dual-label probe chemistries for detection of genetic variants, such as single nucleotide and copy number variants, to give highly reproducible allelic discrimination.

Yes No Lyo-Ready Yes Yes 4x Yes
Lyo-Ready Direct RNA/DNA LAMP Stool, 4x Stool, LAMP Mixes RT-LAMP MDX145 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 5% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.

Yes No Lyo-Ready Yes Yes 4x Yes
Lyo-Ready Direct DNA LAMP Stool, 4x Stool, LAMP Mixes LAMP MDX144 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.

Yes No Lyo-Ready Yes Yes 4x Yes
Lyo-Ready Direct RNA/DNA qPCR Stool, 4x Stool, Lyo-Ready Mix RT-qPCR MDX143 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 5% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.

Yes No Lyo-Ready Yes Yes 4x Yes
Lyo-Ready Direct DNA qPCR Stool, 4x Stool, Lyo-Ready Mix qPCR MDX142 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.

Yes No Lyo-Ready Yes Yes 4x Yes
Air-Dryable Direct RNA/DNA qPCR Stool, 4x Stool, Air-Dryable Mix RT-qPCR MDX141 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 5% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.

Yes Yes Air-Dryable Yes No 4x Yes
Lyo-Ready Direct RNA/DNA LAMP Saliva, 4x Saliva, Sputum, LAMP Mixes RT-LAMP MDX135 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.

Yes No Lyo-Ready Yes Yes 4x Yes
Lyo-Ready Direct DNA LAMP Saliva, 4x Saliva, Sputum, LAMP Mixes LAMP MDX134 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.

Yes No Lyo-Ready Yes Yes 4x Yes
Lyo-Ready Direct RNA/DNA qPCR Saliva, 4x Saliva, Sputum, Lyo-Ready Mix RT-qPCR MDX133 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.

Yes No Lyo-Ready Yes Yes 4x Yes
Lyo-Ready Direct DNA qPCR Saliva, 4x Saliva, Sputum, Lyo-Ready Mix qPCR MDX132 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.

Yes No Lyo-Ready Yes Yes 4x Yes
Air-Dryable Direct RNA/DNA qPCR Saliva, 4x Saliva, Sputum, Air-Dryable Mix RT-qPCR MDX131 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.

Yes Yes Air-Dryable Yes No 4x Yes
Air-Dryable Direct DNA qPCR Saliva, 4x Saliva, Sputum, Air-Dryable Mix qPCR MDX130 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.

Yes Yes Air-Dryable Yes No 4x Yes
Lyo-Ready Genotyping Direct qPCR Blood, 4x Blood, Plasma, Serum, Lyo-Ready Mix SNP Genotyping MDX128 Order a Sample Available in 1,000 Unit or 10,000 Unit aliquots

Inhibitor tolerance to 10% whole blood, 20% plasma. Compatible with all dual-label probe chemistries for detection of genetic variants, such as single nucleotide and copy number variants, to give highly reproducible allelic discrimination.

Yes No Lyo-Ready Yes Yes 4x Yes
Lyo-Ready Direct RNA/DNA LAMP Blood, 4x Blood, Plasma, Serum, LAMP Mixes RT-LAMP MDX125 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 10% whole blood, 10% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.

Yes No Lyo-Ready Yes Yes 4x Yes
Lyo-Ready Direct DNA LAMP Blood, 4x Blood, Plasma, Serum, LAMP Mixes LAMP MDX124 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% whole blood, 20% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.

Yes No Lyo-Ready Yes Yes 4x Yes
Air-Dryable Direct DNA qPCR Blood, 4x Blood, Plasma, Serum, Air-Dryable Mix qPCR MDX092 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% whole blood, 20% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.

Yes Yes Air-Dryable Yes No 4x Yes
Lyo-Ready Direct RNA/DNA qPCR Blood, 4x Blood, Plasma, Serum, Lyo-Ready Mix RT-qPCR MDX123 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 10% whole blood, 10% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.

Yes No Lyo-Ready Yes Yes 4x Yes
Lyo-Ready Direct DNA qPCR Blood, 4x Blood, Plasma, Serum, Lyo-Ready Mix qPCR MDX122 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% whole blood, 20% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.

Yes No Lyo-Ready Yes Yes 4x Yes
Air-Dryable Direct RNA/DNA qPCR Blood, 4x Blood, Plasma, Serum, Air-Dryable Mix RT-qPCR MDX121 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 10% whole blood, 10% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.

Yes Yes Air-Dryable Yes No 4x Yes
Air-Dryable Direct DNA qPCR Plant, 4x Plant, Air-Dryable Mix qPCR MDX116 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

No need for extraction, compatible with 25% Plant lysate (SDS, NaOH). Perfect for multiplexing and fast cycling conditions for in-field qPCR testing. Used for genotyping and transgene or pathogenic detection.

Yes Yes Air-Dryable Yes No 4x Yes

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Sample Specific Inhibitor Tolerant qPCR and RT-qPCR Formulations

In developing universal inhibitor tolerant qPCR and RT-qPCR mixes, a compromise is required to accommodate all of the different types of inhibitors, this reduces sensitivity of the quantitative PCR. Meridian has therefore developed sample specific inhibitor tolerant qPCR and RT-qPCR formulations, designed to be used for direct amplification from crude lysates or inhibitor-rich samples either from blood, saliva, urine or stool. The ability of these mixes to tolerate the high level of PCR inhibitors present in these different sample types allows the development of simpler, faster multiplex qPCR assays that require minimal sample processing and fast turn-around times (TAT). This makes them ideal for liquid biopsy and point of care testing (POCT), where speed, sensitivity, and reproducibility are essential.

The mixes can be used as wet mixes or dried by lyophilization or air-drying, there are several advantages to this, including room temperature shipping and storage, extended shelf-life, and increased flexibility in the sample volume.

Other Specimens Used for Laboratory Diagnostic Testing

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