特定样本类型 Specimen-specific^™ 抗抑性预混液

临床和环境样品中固有的各种不同抑制剂会对分子检测的敏感性和准确性产生负面影响。为了克服这一问题，传统检测方法中样品在检验之前要进行预处理加工。然而，提取技术非100%有效性可能会影响可用于检测的核酸靶标的数量，一些抑制剂可以在纯化后协同洗脱，导致假阴性检测结果。 迈迪安公司推出的抗抑性qPCR/RT-qPCR预混液可用于从粗裂解物或富含抑制物的样本(如痰液、唾液、尿液、粪便或血液)中直接进行免核酸提取扩增。适用于要求快速周转时间或最小样品处理以防止样品丢失的定量分析。

In developing universal inhibitor tolerant qPCR and RT-qPCR mixes, a compromise is required to accommodate all of the different types of inhibitors, this reduces sensitivity of the quantitative PCR. Meridian has therefore developed sample specific inhibitor tolerant qPCR and RT-qPCR formulations, designed to be used for direct amplification from crude lysates or inhibitor-rich samples either from blood, saliva, urine or stool. The ability of these mixes to tolerate the high level of PCR inhibitors present in these different sample types allows the development of simpler, faster multiplex qPCR assays that require minimal sample processing and fast turn-around times (TAT). This makes them ideal for liquid biopsy and point of care testing (POCT), where speed, sensitivity, and reproducibility are essential.

The mixes can be used as wet mixes or dried by lyophilization or air-drying, there are several advantages to this, including room temperature shipping and storage, extended shelf-life, and increased flexibility in the sample volume.