Description
Lyo-Ready AOF dUTP DNA qPCR Blood Mix (MDX322) uses an advanced one-tube formulation, incorporating the latest innovations in buffer chemistry and PCR enhancers, along with an optimized concentration of recombinant antibody-mediated hot-start polymerase, dNTPs (including dUTP), and MgCl2. Lyo-Ready AOF dUTP RNA/DNA qPCR Blood Mix, (MDX323) also includes reverse transcriptase and RNase inhibitor for comprehensive nucleic acid detection.
Lyo-Ready AOF dUTP DNA/RNA qPCR mixes utilize the dUTP/UDGase system to prevent carryover contamination, minimizing false positives and ensuring accurate results, especially in high-throughput workflows. BSA-free and sustainable, these mixes also align with evolving regulatory trends toward in vitro systems, reducing reliance on animal-sourced reagents while offering cost-effective, ambient-temperature stability through their lyophilization-ready formulation.
Blood is one of the most common specimens used for laboratory diagnostic testing and it is useful for evaluating the function of vital organs (kidneys, liver, thyroid, and heart) and for diagnosing diseases such as bacterial and viral infections, cancer, cardiovascular disease, and metabolic disorders such as diabetes. However, inhibitors like immunoglobulin G, hemoglobin and lactoferrin can impact accuracy. Our inhibitor-tolerant Lyo-Ready mixes ensure sensitive RNA/DNA detection, even in crude whole blood, serum and plasma samples, providing reliable results for diverse diagnostic applications.
Key points:
- Supports the development of blood-based screening assays, such as assays detecting cancer or infections.
- Enables highly sensitive detection of low-copy number bacteria, viruses, and cell-free nucleic acids directly from crude whole blood, serum and plasma samples.
- Validated for detection of blood pathogens and liquid biopsy applications.
- Offers the flexibility to remain as a liquid mix or be lyophilized, creating ambient-temperature stable assays with extended shelf life.
Example Protocol
| Reagent Name | Volume |
|---|---|
| Lyo-Ready™ AOF Direct DNA qPCR Blood Mix, 4x | 5 μL |
| Primer-Probe Mix, 20x | 1 μL |
| Water | Up to 10 μL |
Figure 1. Lyo-Ready AOF Direct DNA qPCR Blood Mix Combine reagents as shown for a total reaction volume of 20 μL. Briefly vortex and spin down briefly in a microcentrifuge. Incubate the reaction for 2 minutes at 95°C and then cycle for 5 seconds at 95°C and 20 seconds at 60°C for 45 cycles.
Lyo-Ready AOF dUTP DNA qPCR Blood, MDX322
An optimized qPCR master mix tolerant to inhibitors in whole blood and plasma and compatible with all anticoagulants. Perfect for multiplexing, high throughput assays where there is a greater risk of false positives from cross-over contamination.
Documents & Resources
Lyo-Ready AOF dUTP RNA/DNA qPCR Blood, MDX323
An optimized RT-qPCR master mix tolerant to inhibitors in whole blood and plasma and compatible with all anticoagulants. Perfect for multiplexing, high throughput assays where there is a greater risk of false positives from cross-over contamination.
Documents & Resources
Catalogs & Brochures
Air-Dryable Direct BloodAir-Dryable™ Direct BloodCatalog
Air-Dryable MixesAir-Dryable qPCR/RT-qPCRCatalog
AIR-DRYING MOLECULAR ASSAYSAir-Drying Molecular AssaysQuick Reference Guide
Lyo-Ready Direct DNA LAMP Blood MixesLyo-Ready Direct DNA LAMP Blood MixesCatalog
All-in-One dUTP qPCR Master Mix Optimized for High-Throughput & Multiplex AssaysAll-in-One dUTP qPCR Master Mix Optimized for High-Throughput & Multiplex Assays
FAQs: Lyo-Ready AOF dUTP DNA and RNA/DNA qPCR Blood Mix
Does not contain materials derived from eukaryotic animals.
No, UDGase (Uracil DNA Glycosylase) needs to be added separately where there is a greater risk of false positives from cross-over contamination.
Blood contains abundant information about the health status of the organism, it can be used to evaluate organ function, detect proteins, nucleic acids, metabolites, infectious diseases and can be used for diagnosis of cancer (liquid biopsy), heart disease, diabetes. Blood tests are a simple and easily accessible (low-invasive) method to assess and monitor health.
Blood-based quantitative PCR (qPCR) can detect a wide variety of infectious diseases by identifying the genetic material (DNA or RNA) of pathogens, including viruses, bacteria, and parasites. The technique is highly sensitive and specific, making it an essential tool for diagnosing infectious diseases, such as: Viral Infections, including HIV/AIDS, Epstein-Barr Virus (EBV), Herpes Simplex Virus (HSV) and Human Papillomavirus (HPV). Bacterial Infections, including Tuberculosis (TB), Sepsis (organisms like Staphylococcus aureus, Escherichia coli, or Klebsiella species) and Meningococcal Infections (Neisseria meningitidis DNA in blood, especially in septicemia). Parasitic Infections, including Plasmodium species, Toxoplasma gondii and Leishmaniasis.
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